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  4. Recombinant Human Transketolase (TKT)

Recombinant Human Transketolase (TKT)

  • 货号：
    CSB-EP023580HU
  • 规格：
    ￥3132
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    TKT
  • Uniprot No.：
  • 别名：
    EC 2.2.1.1; FLJ34765; TK; TKT 1; tkt; TKT_HUMAN; TKT1; Transketolase (Wernicke Korsakoff syndrome); Transketolase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    67.9 kDa
  • 表达区域：
    1-623aa
  • 氨基酸序列
    MESYHKPDQQKLQALKDTANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKSQDPRNPHNDRFVLSKGHAAPILYAVWAEAGFLAEAELLNLRKISSDLDGHPVPKQAFTDVATGSLGQGLGAACGMAYTGKYFDKASYRVYCLLGDGELSEGSVWEAMAFASIYKLDNLVAILDINRLGQSDPAPLQHQMDIYQKRCEAFGWHAIIVDGHSVEELCKAFGQAKHQPTAIIAKTFKGRGITGVEDKESWHGKPLPKNMAEQIIQEIYSQIQSKKKILATPPQEDAPSVDIANIRMPSLPSYKVGDKIATRKAYGQALAKLGHASDRIIALDGDTKNSTFSEIFKKEHPDRFIECYIAEQNMVSIAVGCATRNRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIGEDGPSQMALEDLAMFRSVPTSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYNNNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAELLKKEKINIRVLDPFTIKPLDRKLILDSARATKGRILTVEDHYYEGGIGEAVSSAVVGEPGITVTHLAVNRVPRSGKPAELLKMFGIDRDAIAQAVRGLITKA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    Tag-Free
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.
  • 基因功能参考文献：
    1. Over-expressed and hypo-methylated TKT gene is associated with hepatocellular carcinoma. PMID: 27760737
    2. The observed associations of genetic variation in transketolase enzyme with neuropathic symptoms and reduced thermal sensation in recent-onset diabetes suggest a role of pathways metabolizing glycolytic intermediates in early diabetic neuropathy. PMID: 27103086
    3. reduced expression of transketolase in pyrimidine 5'-nucleotidase deficient patients PMID: 27381654
    4. Mutations in TKT gene is associated with a Syndrome Including Short Stature, Developmental Delay, and Congenital Heart Defects. PMID: 27259054
    5. transketolase (TKT)is required for cancer growth because of its ability to affect the production of NAPDH to counteract oxidative stress. PMID: 26811478
    6. Transketolase is upregulated in metastatic peritoneal implants and promotes ovarian cancer cell proliferation. PMID: 25895698
    7. Akt1 phosphorylates TKT on Thr382, markedly enhancing enzyme activity. PMID: 24981175
    8. TKT rs3736156 identified significant differences in the onset of cardiovascular event in patients having genotypes AG + AA versus GG PMID: 23492569
    9. basal TK activity was decreased in cases with diabetic neuropathy PMID: 24114639
    10. Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme. PMID: 22645655
    11. Data indicate that transketolase (hTKT). shares 61% sequence identity with transketolase-like protein (TKTL1). PMID: 23261987
    12. No transketolase activity of TKTDelta38 can be detected for conversion of physiological sugar substrates thus arguing against an intrinsically encoded enzymatic function of TKTL1 in tumor cell metabolism PMID: 23118983
    13. TKT is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
    14. Single Nucleotide Polymorphism in transketolase is associated with diabetic nephropathy. PMID: 20826743
    15. The crystal structure of human transketolase and new insights into its mode of action. PMID: 20667822
    16. Almost all multiple sclerosis patients had cerebrospinal fluid IgG directed to isoforms of one of the oligodendroglial molecules, transketolase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase type I, collapsin response mediator protein 2, and tubulin beta4. PMID: 18676363

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  • 相关疾病：
    Short stature, developmental delay, and congenital heart defects (SDDHD)
  • 蛋白家族：
    Transketolase family
  • 数据库链接：

    HGNC: 11834

    OMIM: 606781

    KEGG: hsa:7086

    STRING: 9606.ENSP00000405455

    UniGene: Hs.89643