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  4. Recombinant Human Transketolase (TKT)

Recombinant Human Transketolase (TKT)

  • 货号：
    CSB-YP023580HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023580HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023580HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023580HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TKT
  • Uniprot No.：
  • 别名：
    EC 2.2.1.1; FLJ34765; TK; TKT 1; tkt; TKT_HUMAN; TKT1; Transketolase (Wernicke Korsakoff syndrome); Transketolase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-623
  • 氨基酸序列
    MESYHKPDQQ KLQALKDTAN RLRISSIQAT TAAGSGHPTS CCSAAEIMAV LFFHTMRYKS QDPRNPHNDR FVLSKGHAAP ILYAVWAEAG FLAEAELLNL RKISSDLDGH PVPKQAFTDV ATGSLGQGLG AACGMAYTGK YFDKASYRVY CLLGDGELSE GSVWEAMAFA SIYKLDNLVA ILDINRLGQS DPAPLQHQMD IYQKRCEAFG WHAIIVDGHS VEELCKAFGQ AKHQPTAIIA KTFKGRGITG VEDKESWHGK PLPKNMAEQI IQEIYSQIQS KKKILATPPQ EDAPSVDIAN IRMPSLPSYK VGDKIATRKA YGQALAKLGH ASDRIIALDG DTKNSTFSEI FKKEHPDRFI ECYIAEQNMV SIAVGCATRN RTVPFCSTFA AFFTRAFDQI RMAAISESNI NLCGSHCGVS IGEDGPSQMA LEDLAMFRSV PTSTVFYPSD GVATEKAVEL AANTKGICFI RTSRPENAII YNNNEDFQVG QAKVVLKSKD DQVTVIGAGV TLHEALAAAE LLKKEKINIR VLDPFTIKPL DRKLILDSAR ATKGRILTVE DHYYEGGIGE AVSSAVVGEP GITVTHLAVN RVPRSGKPAE LLKMFGIDRD AIAQAVRGLI TKA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.
  • 基因功能参考文献：
    1. Over-expressed and hypo-methylated TKT gene is associated with hepatocellular carcinoma. PMID: 27760737
    2. The observed associations of genetic variation in transketolase enzyme with neuropathic symptoms and reduced thermal sensation in recent-onset diabetes suggest a role of pathways metabolizing glycolytic intermediates in early diabetic neuropathy. PMID: 27103086
    3. reduced expression of transketolase in pyrimidine 5'-nucleotidase deficient patients PMID: 27381654
    4. Mutations in TKT gene is associated with a Syndrome Including Short Stature, Developmental Delay, and Congenital Heart Defects. PMID: 27259054
    5. transketolase (TKT)is required for cancer growth because of its ability to affect the production of NAPDH to counteract oxidative stress. PMID: 26811478
    6. Transketolase is upregulated in metastatic peritoneal implants and promotes ovarian cancer cell proliferation. PMID: 25895698
    7. Akt1 phosphorylates TKT on Thr382, markedly enhancing enzyme activity. PMID: 24981175
    8. TKT rs3736156 identified significant differences in the onset of cardiovascular event in patients having genotypes AG + AA versus GG PMID: 23492569
    9. basal TK activity was decreased in cases with diabetic neuropathy PMID: 24114639
    10. Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme. PMID: 22645655
    11. Data indicate that transketolase (hTKT). shares 61% sequence identity with transketolase-like protein (TKTL1). PMID: 23261987
    12. No transketolase activity of TKTDelta38 can be detected for conversion of physiological sugar substrates thus arguing against an intrinsically encoded enzymatic function of TKTL1 in tumor cell metabolism PMID: 23118983
    13. TKT is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
    14. Single Nucleotide Polymorphism in transketolase is associated with diabetic nephropathy. PMID: 20826743
    15. The crystal structure of human transketolase and new insights into its mode of action. PMID: 20667822
    16. Almost all multiple sclerosis patients had cerebrospinal fluid IgG directed to isoforms of one of the oligodendroglial molecules, transketolase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase type I, collapsin response mediator protein 2, and tubulin beta4. PMID: 18676363

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  • 相关疾病：
    Short stature, developmental delay, and congenital heart defects (SDDHD)
  • 蛋白家族：
    Transketolase family
  • 数据库链接：

    HGNC: 11834

    OMIM: 606781

    KEGG: hsa:7086

    STRING: 9606.ENSP00000405455

    UniGene: Hs.89643