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  4. Recombinant Human Ubiquitin carboxyl-terminal hydrolase 30 (USP30), partial

Recombinant Human Ubiquitin carboxyl-terminal hydrolase 30 (USP30), partial

  • 中文名称：
    人USP30重组蛋白
  • 货号：
    CSB-YP765106HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人USP30重组蛋白
  • 货号：
    CSB-EP765106HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人USP30重组蛋白
  • 货号：
    CSB-EP765106HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人USP30重组蛋白
  • 货号：
    CSB-BP765106HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人USP30重组蛋白
  • 货号：
    CSB-MP765106HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Deubiquitinating enzyme 30; FLJ40511; Ub-specific protease 30; Ubiquitin carboxyl terminal hydrolase 30; Ubiquitin carboxyl-terminal hydrolase 30; Ubiquitin specific peptidase 30; Ubiquitin specific processing protease 30; Ubiquitin thioesterase 30; Ubiquitin thiolesterase 30; Ubiquitin-specific-processing protease 30; UBP30_HUMAN; usp30
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2.
  • 基因功能参考文献：
    1. the role of USP30 in the dynamic networks of mitochondrial quality control and its physiological implications. PMID: 29178074
    2. Here the s report crystal structures of human USP30 bound to monoubiquitin and Lys6-linked diubiquitin, which explain how USP30 achieves Lys6-linkage preference through unique ubiquitin binding interfaces. PMID: 28945249
    3. show that USP30 delays mitophagy by delaying PARK2 recruitment to the mitochondria during mitophagy PMID: 25915564
    4. These results provide the first evidence for a fundamental role of USP30 in determining the threshold for mitochondrial cell death and suggest USP30 as a potential target for combinatorial anti-cancer therapy. PMID: 25739811
    5. The mechanism by which parkin, an E3 ubiquitin ligase, and USP30, a mitochondrion-localized deubiquitylase, regulate mitophagy, was investigated. PMID: 25621951
    6. reducing USP30 activity enhances mitochondrial degradation in neurons PMID: 24896179
    7. USP30 participates in the maintenance of mitochondrial morphology, a finding that provides new insight into the cellular function of deubiquitination. PMID: 18287522

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  • 亚细胞定位：
    Mitochondrion outer membrane.
  • 蛋白家族：
    Peptidase C19 family
  • 组织特异性：
    Expressed in skeletal muscle, pancreas, liver and kidney.
  • 数据库链接：

    HGNC: 20065

    OMIM: 612492

    KEGG: hsa:84749

    STRING: 9606.ENSP00000257548

    UniGene: Hs.486434