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  4. Recombinant Human Vesicle-fusing ATPase (NSF)

Recombinant Human Vesicle-fusing ATPase (NSF)

  • 货号：
    CSB-YP341619HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP341619HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP341619HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP341619HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP341619HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor like protein; N ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive fusion protein; NEM sensitive fusion protein; NEM-sensitive fusion protein; NSF; NSF_HUMAN; SKD 2; SKD2; Vesicle fusing ATPase; Vesicle-fusing ATPase; Vesicular fusion protein NSF; Vesicular-fusion protein NSF
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-744
  • 氨基酸序列
    MAGRSMQAAR CPTDELSLTN CAVVNEKDFQ SGQHVIVRTS PNHRYTFTLK THPSVVPGSI AFSLPQRKWA GLSIGQEIEV SLYTFDKAKQ CIGTMTIEID FLQKKSIDSN PYDTDKMAAE FIQQFNNQAF SVGQQLVFSF NEKLFGLLVK DIEAMDPSIL KGEPATGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALLREEGASP LDFD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
  • 基因功能参考文献：
    1. A large copy number variant (CNV) that encompasses part of the NSF gene contributes to cocaine dependence. PMID: 27498889
    2. NSF deficiency in HeLa cells barely affected cell viability, anterograde trafficking of vesicular stomatitis virus glycoprotein G and transferrin endocytosis. PMID: 27995606
    3. a processive helicase-like mechanism for NSF in which approximately 1 residue is unwound for every hydrolyzed ATP molecule. PMID: 23775070
    4. Single and multiple-marker analyses revealed a strong association between cocaine dependence and the NSF gene. PMID: 21426264
    5. Knockdown of TRX1 increases the level of S-nitrosylated NSF, prolongs the inhibition of exocytosis, and suppresses leukocyte PMID: 21324905
    6. NSF independent fusion of Salmonella-containing late phagosomes with early endosomes. PMID: 20176016
    7. analysis of human NSF possible binding mode to GABARAP and GATE-16 PMID: 19533740
    8. binding of NSF to GluR2-containing AMPARs in stabilizing these receptors in the synaptic membrane and impeding their regulated endocytosis. PMID: 12011465
    9. role of interaction with GluR2 in regulation of AMPA receptors by brain-derived neurotrophic factor PMID: 12130635
    10. Nitric oxide regulates exocytosis by S-nitrosylation of NSF. PMID: 14567912
    11. Expressing a biochemically defined NSF mutant that lacks ATPase activity results in a dramatic inhibition of stimulated mast cell exocytosis. PMID: 14607937
    12. NSF dissociates the VAMP-2.SNAP-25.syntaxin 13 complex, but has no effect on the Hrs-containing complex. PMID: 14769786
    13. Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein PMID: 16461345
    14. dual regulation on hbeta2AR trafficking and signaling by NSF through direct binding to cargo receptor and its ATPase activity. PMID: 17510209
    15. Data show that NSF is an inclusion body component in neuronal intranuclear inclusion disease identified by anti-SUMO-1-immunocapture. PMID: 18836734
    16. Data show that phosphorylation of N-ethylmaleimide-sensitive factor on tyrosine residues prevents its SNARE complex dissociation activity and establish a role for PTP1B in the modulation of the membrane fusion machinery. PMID: 19208619

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    AAA ATPase family
  • 数据库链接：

    HGNC: 8016

    OMIM: 601633

    KEGG: hsa:4905

    STRING: 9606.ENSP00000381293

    UniGene: Hs.431279