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  4. Recombinant Human Zinc finger protein 350 (ZNF350)

Recombinant Human Zinc finger protein 350 (ZNF350)

  • 货号：
    CSB-YP026694HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026694HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026694HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026694HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026694HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ZNF350
  • Uniprot No.：
  • 别名：
    KRAB zinc finger protein ZFQR; ZBRK1; ZFQR; Zinc finger and BRCA1 interacting protein with a KRAB domain 1; Zinc finger and BRCA1-interacting protein with a KRAB domain 1; Zinc finger protein 350; Zinc finger protein ZBRK1; ZN350_HUMAN; ZNF350
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-532
  • 氨基酸序列
    MIQAQESITLEDVAVDFTWEEWQLLGAAQKDLYRDVMLENYSNLVAVGYQASKPDALFKL EQGEQLWTIEDGIHSGACSDIWKVDHVLERLQSESLVNRRKPCHEHDAFENIVHCSKSQF LLGQNHDIFDLRGKSLKSNLTLVNQSKGYEIKNSVEFTGNGDSFLHANHERLHTAIKFPA SQKLISTKSQFISPKHQKTRKLEKHHVCSECGKAFIKKSWLTDHQVMHTGEKPHRCSLCE KAFSRKFMLTEHQRTHTGEKPYECPECGKAFLKKSRLNIHQKTHTGEKPYICSECGKGFI QKGNLIVHQRIHTGEKPYICNECGKGFIQKTCLIAHQRFHTGKTPFVCSECGKSCSQKSG LIKHQRIHTGEKPFECSECGKAFSTKQKLIVHQRTHTGERPYGCNECGKAFAYMSCLVKH KRIHTREKQEAAKVENPPAERHSSLHTSDVMQEKNSANGATTQVPSVAPQTSLNISGLLA NRNVVLVGQPVVRCAASGDNRGFAQDRNLVNAVNVVVPSVINYVLFYVTENP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor. Binds to a specific sequence, 5'-GGGxxxCAGxxxTTT-3', within GADD45 intron 3.
  • 基因功能参考文献：
    1. Results indicate ZNF350 as an important gene mammary oncogenesis. PMID: 29653063
    2. Results indicated that SNP rs2278414 at ZNF350 may in fl uence an individual's susceptibility to age-related cataract (ARC) by affecting the binding af fi nity of miR-21-3p and miR-150-5p and regulating expression levels of the mRNAs, resulting in different levels of cellular DNA breaks, thus contributing to ARC. PMID: 27586871
    3. villin directly interacts with a transcriptional corepressor and ligand of the Slug promoter, ZBRK1. PMID: 26658611
    4. ZBRK1 suppresses renal cancer progression perhaps by regulating VHL expression through formation of a complex with VHL and p300 in renal cancer PMID: 25749518
    5. MAGE proteins bind to KAP1, a gene repressor and ubiquitin E3 ligase which also binds KRAB domain containing zinc finger transcription factors (KZNFs), and MAGE expression may affect KZNF mediated gene regulation. PMID: 25107531
    6. The secondary structure of the ZBRK1-DNA complex is found to be significantly altered from the standard B-DNA conformation. PMID: 24924633
    7. results indicate that a loss of ZBRK1 contributes to the increased expression of KAP1, potentiating its role to enhance metastasis and invasion PMID: 23991171
    8. promoter assays in two breast cancer cell lines identified two haplotypes (H11 and H12) stimulating significantly the expression of ZNF350 transcript compared with the common haplotype H8 PMID: 23151675
    9. ZBRK1 negatively regulates the HIV-1 LTR PMID: 22975076
    10. high levels of gene activity in the genomic environment and the pre-deposition of repressive histone marks within a gene increase its susceptibility to KRAB/KAP1-mediated repression PMID: 21791101
    11. KRAB-containing zinc-finger transcriptional regulator, ZBRK1, an interaction partner of the SCA2 gene product ataxin-2. PMID: 20926453
    12. RB.E2F1 complex plays a critical role in ZBRK1 transcriptional repression, and loss of this repression may contribute to cellular sensitivity of DNA damage, ultimately leading to carcinogenesis. PMID: 20713352
    13. Data found that HMGA2, along with a dozen of other genes, was co-repressed by ZBRK1, BRCA1, and CtIP. PMID: 20007691
    14. KRAB/KAP1 recruitment induces long-range repression through the spread of heterochromatin. PMID: 20221260
    15. Our findings suggest that ZBRK1 acts to inhibit metastasis of cervical carcinoma, perhaps by modulating MMP9 expression. PMID: 19996286
    16. ZBRK1 mutation found in BRCA1 and BRCA2 mutation negative probands with breast or ovarian cancer. PMID: 12872252
    17. ZBRK1 is a novel target for DNA damage-induced degradation PMID: 14517299
    18. ZBRK1 zinc fingers have dual roles in sequence-specific DNA-binding and BRCA1-dependent transcriptional repression PMID: 14660588
    19. the CTRD is a novel protein interaction surface responsible for directing homotypic and heterotypic interactions necessary for ZBRK1-directed transcriptional repression PMID: 15496401
    20. interacts with BBLF2/3 to provide a tethering point on oriLyt for the EBV replication complex PMID: 15596820
    21. KRAB-induced transcriptional repression is robust and active over a variety of genomic contexts that include at least the wide range of sites targeted by lentiviral integration PMID: 16997916
    22. Genetic variants and haplotypes of the ZBRK1 gene is associated with breast and ovarian cancer PMID: 17764113
    23. KRAB/KAP1 regulation is fully functional within the context of episomal DNA PMID: 19279087
    24. BRCA1 and ZNF350 may jointly contribute to individuals' susceptibility of breast cancer in Chinese women. PMID: 19484476

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  • 亚细胞定位：
    Nucleus. Nucleus matrix. Note=Associated with the nuclear matrix.
  • 蛋白家族：
    Krueppel C2H2-type zinc-finger protein family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 16656

    OMIM: 605422

    KEGG: hsa:59348

    STRING: 9606.ENSP00000243644

    UniGene: Hs.407694