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  4. Recombinant Human Zinc finger protein DPF3 (DPF3)

Recombinant Human Zinc finger protein DPF3 (DPF3)

  • 中文名称：
    人DPF3重组蛋白
  • 货号：
    CSB-YP849783HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人DPF3重组蛋白
  • 货号：
    CSB-EP849783HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人DPF3重组蛋白
  • 货号：
    CSB-EP849783HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人DPF3重组蛋白
  • 货号：
    CSB-BP849783HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人DPF3重组蛋白
  • 货号：
    CSB-MP849783HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DPF3
  • Uniprot No.：
  • 别名：
    2810403B03Rik; BAF45C; BRG1-associated factor 45C; cer-d4 homolog; CERD4; D4 zinc and double PHD fingers family member 3; D4; zinc and double PHD fingers; family 3; DPF 3; Dpf3; DPF3_HUMAN; FLJ 14079; FLJ14079; Mouse homolog of CER D4; Zinc finger protein cer-d4; Zinc finger protein DPF3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-378
  • 氨基酸序列
    MATVIHNPLK ALGDQFYKEA IEHCRSYNSR LCAERSVRLP FLDSQTGVAQ NNCYIWMEKR HRGPGLAPGQ LYTYPARCWR KKRRLHPPED PKLRLLEIKP EVELPLKKDG FTSESTTLEA LLRGEGVEKK VDAREEESIQ EIQRVLENDE NVEEGNEEED LEEDIPKRKN RTRGRARGSA GGRRRHDAAS QEDHDKPYVC DICGKRYKNR PGLSYHYAHT HLASEEGDEA QDQETRSPPN HRNENHRPQK GPDGTVIPNN YCDFCLGGSN MNKKSGRPEE LVSCADCGRS GHPTCLQFTL NMTEAVKTYK WQCIECKSCI LCGTSENDDQ LLFCDDCDRG YHMYCLNPPV AEPPEGSWSC HLCWELLKEK ASAFGCQA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.
  • 基因功能参考文献：
    1. DPF3 single nucleotide polymorphisms were significantly correlated with male infertility in a Japanese population. PMID: 28975488
    2. SLC1A1 and DPF3 were strongly associated with idiopathic male infertility and were significantly correlated with semen quality alterations PMID: 27232852
    3. the high resolution crystal structure of the tandem PHD fingers of DPF3b in complex with an H3K14ac peptide. PMID: 27402533
    4. Activation of DPF3a upon hypertrophic stimuli in cardiac hypertrophy switches cardiac fetal gene expression from being silenced by HEY to being activated by BRG1. PMID: 26582913
    5. Immunohistochemical staining showed that intensive DPF3 staining occurred in proximal anastomosis and the positive staining was hardly observed in stenotic segment in colon specimens from patients with Hirschsprung's disease PMID: 25120773
    6. DFP3 protein rs10129954 SNP is associated with poor sperm morphology. PMID: 24271036
    7. These findings provide insights in the STAT5 dependent transcriptional regulation of Dpf3. PMID: 24155890
    8. by linking RelA and p50 to the SWI/SNF complex, DPF3a/b induces the transactivation of NF-kappaB target gene promoters in relatively inactive chromatin contexts. PMID: 22334708
    9. three-dimensional solution structures and biochemical analysis of DPF3b highlight the molecular basis of the integrated tandem PHD finger, which acts as one functional unit in the sequence-specific recognition of lysine-14-acetylated histone H3 PMID: 20613843
    10. DPF3 adds a further layer of complexity to the BAF complex by representing a tissue-specific anchor between histone acetylations as well as methylations and chromatin remodeling. PMID: 18765789

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Requiem/DPF family
  • 数据库链接：

    HGNC: 17427

    OMIM: 601672

    KEGG: hsa:8110

    STRING: 9606.ENSP00000370614

    UniGene: Hs.162868