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  4. Recombinant Listeria monocytogenes serovar 1/2a Peptide chain release factor 1 (prfA)

Recombinant Listeria monocytogenes serovar 1/2a Peptide chain release factor 1 (prfA)

  • 中文名称：
    单核增生李斯特菌血清1/2aprfA重组蛋白
  • 货号：
    CSB-YP835003LPY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    单核增生李斯特菌血清1/2aprfA重组蛋白
  • 货号：
    CSB-EP835003LPY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    单核增生李斯特菌血清1/2aprfA重组蛋白
  • 货号：
    CSB-EP835003LPY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    单核增生李斯特菌血清1/2aprfA重组蛋白
  • 货号：
    CSB-BP835003LPY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    单核增生李斯特菌血清1/2aprfA重组蛋白
  • 货号：
    CSB-MP835003LPY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    prfA
  • Uniprot No.：
  • 别名：
    prfA; prf1; lmo2543; Peptide chain release factor 1; RF-1
  • 种属：
    Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-358
  • 氨基酸序列
    MYDRLQAVED RYDELNELLS DPDVVSDPKR LRDLSKEQSG ITATVETYRE YKNVNEQINE TKELLGEKLD DEMREMAKEE FAELQKEKTD LEERLKLLLV PKDPNDDKNV ILEIRGAAGG DEAALFAGDL FRMYSKYAES RGWKVEIMDA NPTGIGGYKE IIAMMNGNDA FSRMKYENGA HRVQRVPETE SGGRIHTSTA TVAILPEAEE VEIELHDKDI RTDTFASTGA GGQSVNTTMS AVRLTHIPTG IVVSMQDERS QLKNKDKAMK VLRARVYDKF EREAREEYDA NRKSAVGTGD RSERIRTYNY PQNRVTDHRI GLTIQKLDQI MEGKLDEIID ALILEDQTSK LEHLNDAN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.
  • 基因功能参考文献：
    1. By binding to PrfA in the cytosol of the host cell, GSH induces the correct fold of the HTH motifs, thus priming the PrfA protein for DNA interaction. PMID: 27930316
    2. Structural insights into glutathione-mediated activation of the master regulator PrfA in Listeria monocytogenes. PMID: 28271443
    3. Using a mutant PrfA regulator locked ON (PrfA*) and thus causing PrfA- controlled genes to be constitutively activated, the constitutively virulent state strongly impaired L. monocytogenes performance in soil, the natural habitat of these bacteria. PMID: 26178789
    4. Data show that a mutation that renders the virulence gene activator PrfA constitutively active not only affects the phosphorylation level of numerous proteins but also allowed the detection of about 100 novel phosphoproteins. PMID: 25383790
    5. Constitutive activation of PrfA potentiated virulence of the pathogenic strain EGDe, shown as increased adhesion and invasion as well as enhanced cell-to-cell spread in cultured cell lines. PMID: 26055558
    6. These data indicated that loss of PrfA dramatically altered gene expression patterns in Listeria monocytogenes biofilm and resulted in reduced ability of the biofilm formation. PMID: 23652633
    7. The first 20 codons of the prfA mRNA is required to be maintained in a flexible manner, to allow ribosome binding and translation initiation. PMID: 22053088
    8. The prfA P219S mutation mutation alters PrfA conformation which reduces dimer formation, enhances DNA-binding affinity, and results in constitutive activation of PrfA. PMID: 21835879
    9. These results suggest that PrfA plays a significant role in biofilm formation in Listeria monocytogenes but not in Listeria innocua. PMID: 21656247
    10. PrfA activation tilts the balance of Listeria monocytogenes fitness towards life within the host versus environmental survival PMID: 21151923
    11. PrfA, is required for the chitinolytic phenotype, as chitinase activity was significantly reduced in prfA mutant cells compared to its level in wild-type cells. PMID: 20675445
    12. These and other data suggest that the phosphorylation state of PTS permeases correlates with PrfA activity. PMID: 18502850
    13. Regulatory interactions between PrfA and sigma(B) contribute to PrfA's predominant role as a direct regulator of virulence and Sigma(B) regulates a wider range of virulence and stress response genes. PMID: 18564909
    14. sigmaB and PrfA differentially regulate transcription of internalin and internalin-like genes. PMID: 18713061
    15. PrfA activity is modulated by culture conditions correlates with the expression level and the phosphorylation state of specific PTS permeases. PMID: 19047753

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Prokaryotic/mitochondrial release factor family
  • 数据库链接：

    KEGG: lmo:lmo2543

    STRING: 169963.lmo2543