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  4. Recombinant Mouse AT-rich interactive domain-containing protein 3A (Arid3a)

Recombinant Mouse AT-rich interactive domain-containing protein 3A (Arid3a)

  • 中文名称：
    Recombinant Mouse AT-rich interactive domain-containing protein 3A(Arid3a),Yeast
  • 货号：
    CSB-YP720301MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse AT-rich interactive domain-containing protein 3A(Arid3a),Yeast
  • 货号：
    CSB-EP720301MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse AT-rich interactive domain-containing protein 3A(Arid3a),Yeast
  • 货号：
    CSB-EP720301MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse AT-rich interactive domain-containing protein 3A(Arid3a),Yeast
  • 货号：
    CSB-BP720301MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse AT-rich interactive domain-containing protein 3A(Arid3a),Yeast
  • 货号：
    CSB-MP720301MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Arid3a
  • Uniprot No.：
  • 别名：
    Arid3a; Dri1; Dril1AT-rich interactive domain-containing protein 3A; ARID domain-containing protein 3A; B-cell regulator of IgH transcription; Bright; Dead ringer-like protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-601
  • 氨基酸序列
    MKLQAVMETL IQRQQRARQE LEARQAPPPP PPEPTGVRAR TTMTDEDREP ENARMHRTQM AALAAMRAAA AGLGHPSSPG GSEDGPPISG DEDTAREGTL SSPALHGSVL EGAGHAEGDR HLMDVGSDDD DTKSKWEEQE LEELGEEEEE EEEEDDFEEE EEEEEGLGPP ESASLGTAGL FTRKAPPAQA FRGDGGPRML SGPERLGPGP AHPSHMASQM PPPDHGDWTF EEQFKQLYEL DADPKRKEFL DDLFSFMQKR GTPVNRIPIM AKQVLDLFML YVLVTEKGGL VEVINKKLWR EITKGLNLPT SITSAAFTLR TQYMKYLYPY ECERRGLSSP NELQAAIDSN RREGRRQSFG GSLFAYSPSG AHSMLPSPKL PVTPLGLAAS TNGSSITPAP KIKKEEDSAI PITVPGRLPV SLAGHPVVAA QAAAVQAAAA QAAVAAQAAA LEQLREKLES TEPPEKKMAL VADEQQRLMQ RAVQQSFLAM TAQLPMNIRI NSQASESRQD SAVSLTSANG SNSISMSVEM NGIVYTGVLF AQPPPPTAPS APGKGGVSSI GTNTTTGSRT GASGSTVSGG QVGLPGVSTP TMSSTSNNSL P
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor involved in B-cell differentiation. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. May be involved in the control of cell cycle progression by the RB1/E2F1 pathway.
  • 基因功能参考文献：
    1. Our data show that signals provided by thymic epithelial cells control thymic B cell development by up-regulating Let-7, suppressing Arid3a expression in intrathymic progenitor B cells to limit their proliferation during the neonatal to adult transition. PMID: 29462197
    2. ARID3A is required for normal murine trophoblast development in vivo. The results reveal an essential, conserved function for ARID3A in mammalian placental development through regulation of both intrinsic and extrinsic developmental programs. PMID: 27965054
    3. identify the Arid3a transcription factor as a key target of Let-7, whose ectopic expression is sufficient to induce B-1 development in adult pro-B cells and whose silencing by knockdown blocks B-1 development in fetal pro-B cells. PMID: 25753579
    4. These data reveal new functions for ARID3a in early hematopoiesis and suggest that knowledge regarding ARID3a levels in HSPCs could be informative for applications requiring transplantation of those cells. PMID: 25535283
    5. identify Arid3a as a critical regulator of TE and placental development through execution of the commitment and differentiation phases of the first cell fate decision PMID: 25319825
    6. These data suggest a novel role for Bright in the normal development of mature B cell subsets and in autoantibody production PMID: 21963220
    7. these results place Bright/Arid3a on a select list of transcriptional regulators required to program both hematopoietic stem cell and lineage-specific differentiation. PMID: 21199920
    8. Id1 inhibited DNA binding by Dril1, and the two proteins co-localized in vitro and in vivo, providing a potential mechanism for suppression of fibrosis by Id1 through inhibition of the profibrotic function of Dril1.(Dril1) PMID: 18583319
    9. Bright/ARID3a inhibition causes increased developmental plasticity in mouse and human cells. PMID: 20680960
    10. Variations in Bright binding and matrix attachment region activity contribute to localized control of accessibility and therefore nonrandom gene use during V(D)J recombination. PMID: 12193717
    11. identify regions within Bright that are required for the DNA binding activity of Bright and for its function as a transcription factor PMID: 15456761
    12. The ability of Bright to enhance immunoglobulin transcription critically requires functional Bruton's tyrosine kinase . PMID: 15743806
    13. For IgH transactivation, Bright binds to nuclear matrix association regions.Bright actively shuttles between the nucleus and the cytoplasm, regulation of Bright's cellular localization appears to be required for its function. PMID: 16507996
    14. Bright functions in a subset of Bruton's tyrosine kinase-dependent pathways in vivo, particularly those responses dominated by B1 B cells. PMID: 18981111
    15. A palmitoylated pool of Bright is diverted to lipid rafts of resting B cells where it associates with signalosome components. PMID: 19214191

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Shuttles between nucleus and cytoplasm.
  • 组织特异性：
    B-cell specific in the adult. Expressed in B-cell progenitors, down-regulated in the immature B-cell stage, and is up-regulated again at later stages of B-lymphocyte differentiation.
  • 数据库链接：

    KEGG: mmu:13496

    STRING: 10090.ENSMUSP00000019708

    UniGene: Mm.301282