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  4. Recombinant Mouse ATP-dependent RNA helicase DDX25 (Ddx25)

Recombinant Mouse ATP-dependent RNA helicase DDX25 (Ddx25)

  • 中文名称：
    Recombinant Mouse ATP-dependent RNA helicase DDX25(Ddx25),Yeast
  • 货号：
    CSB-YP879450MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse ATP-dependent RNA helicase DDX25(Ddx25),Yeast
  • 货号：
    CSB-EP879450MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse ATP-dependent RNA helicase DDX25(Ddx25),Yeast
  • 货号：
    CSB-EP879450MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse ATP-dependent RNA helicase DDX25(Ddx25),Yeast
  • 货号：
    CSB-BP879450MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse ATP-dependent RNA helicase DDX25(Ddx25),Yeast
  • 货号：
    CSB-MP879450MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ddx25
  • Uniprot No.：
  • 别名：
    Ddx25; GrthATP-dependent RNA helicase DDX25; EC 3.6.4.13; DEAD box protein 25; Gonadotropin-regulated testicular RNA helicase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-484
  • 氨基酸序列
    MASLLWGGDA GAAESERLNS HFSNLVHPRK NLRGIRSTTV PNIDGSLNTE DDDDDEDDVV DLAANSLLNK LIRQSLIESS HRVEVLQKDP SSPLYSVKTF EELRLKEELL KGIYAMGFNR PSKIQEMALP MMLAHPPQNL IAQSQSGTGK TAAFVLAMLS RVNALELFPQ CLCLAPTYEL ALQTGRVVER MGKFCVDVEV MYAIRGNRIP RGTEVTKQII IGTPGTVLDW CFKRKLIDLT KIRVFVLDEA DVMIDTQGFS DQSIRIQRAL PSECQMLLFS ATFEDSVWQF AERIIPDPNV IKLRKEELTL NNIRQYYVLC ENRKGKYQAL CNIYGGITIG QAIIFCQTRR NAKWLTVEMM QDGHQVSLLS GELTVEQRAS IIQRFRDGKE KVLITTNVCA RGIDVKQVTI VVNFDLPVNQ SEEPDYETYL HRIGRTGRFG KKGLAFNMIE VDKLPLLMKI QDHFNSNIKQ LDPEDMDEIE KIEY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development.
  • 基因功能参考文献：
    1. Data indicate that the transgenic line can be used to define requirements for cell-specific expression of the RNA helicase (GRTH/DDX25) gene. PMID: 23546600
    2. The results suggest that DDX25 functions in meiotic cells,intermitochondrial cement,loose aggregates of particles,chromatoid bodies,late chromatoid bodies,satellite bodies,granulated bodies,mitochondria-associated granules and reticulated bodies. PMID: 22038044
    3. Data show the regulatory pathways underlying GRTH action in male reproduction. PMID: 22479328
    4. GRTH regulation by androgen in Leydig cells; molecular mechanism of androgen-regulated transcription PMID: 22331472
    5. demonstrates that GRTH is essential for completion of spermatogenesis, (ii) provides insights into intrinsic requirements for spermiogenesis, and (iii) establishes a model for studies of male infertility and contraception PMID: 15096601

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids.
  • 蛋白家族：
    DEAD box helicase family
  • 组织特异性：
    Isoform 1 is expressed in germ cells. Isoform 2 is expressed in Leydig cells and in round spermatids of adult testis upon gonadotropin stimulation.
  • 数据库链接：

    KEGG: mmu:30959

    STRING: 10090.ENSMUSP00000034612

    UniGene: Mm.291723