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  4. Recombinant Mouse All-trans-retinol 13,14-reductase (Retsat)

Recombinant Mouse All-trans-retinol 13,14-reductase (Retsat)

  • 中文名称：
    小鼠Retsat重组蛋白
  • 货号：
    CSB-YP714526MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Retsat重组蛋白
  • 货号：
    CSB-EP714526MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Retsat重组蛋白
  • 货号：
    CSB-EP714526MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Retsat重组蛋白
  • 货号：
    CSB-BP714526MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Retsat重组蛋白
  • 货号：
    CSB-MP714526MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Retsat
  • Uniprot No.：
  • 别名：
    Retsat; Ppsig; All-trans-retinol 13,14-reductase; EC 1.3.99.23; All-trans-13,14-dihydroretinol saturase; RetSat; PPAR-alpha-regulated and starvation-induced gene protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-609
  • 氨基酸序列
    ASSPN PFAEDVKRPP EPLVTDKEAR KKVLKQAFSV SRVPEKLDAV VIGSGIGGLA SAAVLAKAGK RVLVLEQHTK AGGCCHTFGE NGLEFDTGIH YIGRMREGNI GRFILDQITE GQLDWAPMAS PFDLMILEGP NGRKEFPMYS GRKEYIQGLK KKFPKEEAVI DKYMELVKVV ARGVSHAVLL KFLPLPLTQL LSKFGLLTRF SPFCRASTQS LAEVLQQLGA SRELQAVLSY IFPTYGVTPS HTAFSLHALL VDHYIQGAYY PRGGSSEIAF HTIPLIQRAG GAVLTRATVQ SVLLDSAGRA CGVSVKKGQE LVNIYCPVVI SNAGMFNTYQ HLLPETVRHL PDVKKQLAMV RPGLSMLSIF ICLKGTKEDL KLQSTNYYVY FDTDMDKAME RYVSMPKEKA PEHIPLLFIA FPSSKDPTWE ERFPDRSTMT ALVPMAFEWF EEWQEEPKGK RGVDYETLKN AFVEASMSVI MKLFPQLEGK VESVTGGSPL TNQYYLAAPR GATYGADHDL ARLHPHAMAS IRAQTPIPNL YLTGQDIFTC GLMGALQGAL LCSSAILKRN LYSDLQALGS KVKAQKKKM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the saturation of all-trans-retinol to all-trans-13,14-dihydroretinol. Does not exhibit any activity toward all-trans-retinoic acid, nor 9-cis, 11-cis or 13-cis-retinol isomers. May play a role in the metabolism of vitamin A. Independently of retinol conversion, may regulate liver metabolism upstream of MLXIPL/ChREBP. Required for adipocyte differentiation in a 3T3-L1 cell culture model. This effect seems not to mimic the in vivo situation in which animals show increased adiposity in the absence of RETSAT.
  • 基因功能参考文献：
    1. Retsat modulates lipid metabolism and the production of reactive oxygen species. PMID: 28927883
    2. Increased adiposity in the retinol saturase-knockout mouse. PMID: 19940255
    3. RetSat-deficiency is associated with alterations in lipid metabolism and the in vivo adipogenic program. PMID: 19940255
    4. Mouse RetSat catalyzes the stereospecific saturation of the C13-C14 double bond of all-trans-retinol to produce (13R)-all-trans-13,14-dihydroretinol. PMID: 18179220
    5. suggest a novel role for retinol saturase in regulating sensitivity to oxidative stress PMID: 17664141
    6. Here, we report the identification and characterization of Ppsig (for PPARalpha-regulated and starvation-induced gene) with unknown biological function from mouse liver PMID: 18289917
    7. RetSat plays an important role in the biology of adipocytes, where it favors normal differentiation, yet is reduced in the obese state. PMID: 19139408

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein.
  • 蛋白家族：
    Carotenoid/retinoid oxidoreductase family, CrtISO subfamily
  • 组织特异性：
    Predominantly expressed in the liver (at protein level). Also expressed at high levels in kidney, intestine, and white fat and brown fat. Weakly expressed in heart, skeletal muscle and testis and barely detected in the lung, brain and spleen. Up-regulated
  • 数据库链接：

    KEGG: mmu:67442

    STRING: 10090.ENSMUSP00000068568

    UniGene: Mm.305108