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  4. Recombinant Mouse Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (Mgat5), partial

Recombinant Mouse Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (Mgat5), partial

  • 中文名称：
    小鼠Mgat5重组蛋白
  • 货号：
    CSB-YP819191MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Mgat5重组蛋白
  • 货号：
    CSB-EP819191MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Mgat5重组蛋白
  • 货号：
    CSB-EP819191MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Mgat5重组蛋白
  • 货号：
    CSB-BP819191MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Mgat5重组蛋白
  • 货号：
    CSB-MP819191MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mgat5
  • Uniprot No.：
  • 别名：
    Mgat5Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; EC 2.4.1.155; Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; GlcNAc-T V; GNT-V; Mannoside acetylglucosaminyltransferase 5; N-acetylglucosaminyl-transferase V) [Cleaved into: Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; Secreted beta-1,6-N-acetylglucosaminyltransferase V; Secreted GNT-V)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2. Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration. MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N-glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation. Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis.; Promotes proliferation of umbilical vein endothelial cells and angiogenesis, at least in part by promoting the release of the growth factor FGF2 from the extracellular matrix.
  • 基因功能参考文献：
    1. Deficiency in Branched Glycosylation in MGAT5 Null or Heterozygous Mice Is Associated with Early-Onset Disease and Increased Severity of Colitis> PMID: 29720442
    2. Study indicates that Mgat5 genotype affects both behaviour and physical outcomes in response to early life stress, suggesting some shared pathways for both in a mouse model. These results provide a starting point for studying the mechanisms by which protein N-glycosylation mediates the effects of early life adversity. PMID: 27329152
    3. Overexpression of GnT-V exacerbated murine experimental colitis by inducing macrophage dysfunction, thereby enhancing colorectal tumorigenesis PMID: 26349931
    4. Expression of GnT-V was also elevated in bleomycin (BLM)-injected sclerotic skin, and MGAT5(-/-) mice were resistant to BLM-induced skin sclerosis with reduced collagen type 1 alpha1 content. PMID: 25876794
    5. GnT-V regulates the canonical Wnt/beta-catenin signaling pathway by modulating N-glycosylation of Wnt receptors, which changes cancer stem cells in colorectal tumors, causing altered colon tumorigenesis and adenoma progression in Apc(min/+) mice PMID: 25274627
    6. this study suggests that the GnT-V expression is inversely correlated with radiation sensitivity in prostate cancer(PCa)cells PMID: 25117443
    7. The GnT-V prevented steatohepatitis progression through modulating lymphocyte and HSC functions. PMID: 22294551
    8. GnT-V overexpression promotes EMT and keratinocyte migration in part through enhanced EGF receptor signaling. PMID: 21697088
    9. Mgat5-dependent glycosylation of proteins can modulate acquired immune responses, but it is not essential for the development of OVA-induced eosinophilic airway inflammation. PMID: 21502802
    10. GnT-V expression and its branched glycan products effectively modulate her-2-mediated signaling pathways that, in turn, regulate the relative proportion of tumor initiating cells and the latency of her-2-driven tumor onset PMID: 21078982
    11. A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V distinct from the original glycosyltransferase activity. PMID: 11872751
    12. reporting of the sequence of mRNA PMID: 12122020
    13. N-linked beta(1,6) branching has a role in cell-cell adhesion, cellular motility and neoplasm invasiveness PMID: 14561752
    14. expression of Mgat5 sensitized cells to multiple cytokines;Galectin-3 cross-linked Mgat5-modified N-glycans on epidermal growth factor and transforming growth factor-beta receptors at the cell surface and delayed their removal by constitutive endocytosis PMID: 15459394
    15. fibroblast N-acetylglucosaminyltransferase V regulates alpha5beta1 integrin expression mediated by the protein kinase C signaling pathway PMID: 15615721
    16. Mgat5 and Pten interact in an opposing manner to regulate cellular sensitivities to extracelluar growth cues PMID: 17400585
    17. Mgat5 modification of complex-type N-glycans on CNS glycoproteins is involved in the regulation of depression-like behavior. as seen in knockout mice. PMID: 17883406
    18. GnT-V and beta1, 6GlcNAc branching mediate the cell migration and invasion in Rac1-positive and RhoA-negative regulatory manners. PMID: 18060576
    19. Blocking expression of Mgat5-modified complex N-glycans in mammary cancer cells using Mgat5 short hairpin RNA suppresses tumor progression both in vivo and in vitro and activates CD4-positive T cells and macrophages. PMID: 18292539
    20. tyrosinase as a beta1,6 branch containing glycoprotein: substrate of GnT-V PMID: 18655794

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  • 亚细胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein. Perikaryon.; [Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A]: Secreted.
  • 蛋白家族：
    Glycosyltransferase 18 family
  • 组织特异性：
    Detected in cerebellum.
  • 数据库链接：

    KEGG: mmu:107895

    STRING: 10090.ENSMUSP00000038359

    UniGene: Mm.214682