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  4. Recombinant Mouse Apelin (Apln)

Recombinant Mouse Apelin (Apln)

  • 货号：
    CSB-YP001909MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001909MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001909MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001909MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001909MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Apln; ApelApelin; APJ endogenous ligand) [Cleaved into: Apelin-36; Apelin-31; Apelin-28; Apelin-13]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    42-77
  • 氨基酸序列
    LVKPRTSRT GPGAWQGGRR KFRRQRPRLS HKGPMPF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

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靶点详情

  • 功能：
    Endogenous ligand for the apelin receptor (APLNR). Drives internalization of APLNR. Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR. Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis. Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates. Plays a role in early coronary blood vessels formation. Mediates myocardial contractility in an ERK1/2-dependent manner. May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior.
  • 基因功能参考文献：
    1. apelin effects on dynamic mechanical characteristics of single ventricular cardiomyocytes PMID: 28935153
    2. Study shows that apelin-13 ameliorates chronic normobaric hypoxia-induced anxiety-like behavior in mice. Anxiolytic effects of apelin-13 might be associated with an inhibition of NF-kappaB and microglial activation in the hippocampus. PMID: 28065733
    3. Results revealed that apelin-13 attenuated brain edema and reduced cellular death by suppressing apoptosis after intracerebral hemorrhage in mice. PMID: 27343409
    4. miR-503 promotes cardiac fibrosis via miR-503-Apelin-13-TGF-beta-CTGF-collagen production pathway. PMID: 26756969
    5. Apelin directly interferes with thrombin-mediated signaling pathways and platelet activation, secretion, and aggregation, but not with ADP and thromboxane A2-mediated pathways. PMID: 26634301
    6. Apelin regulates inflammatory response, diminishes inflammatory remote organ damage and improves survival in an experimental model of burn sepsis, which is at least partly mediated by a phosphatidylinositol 3-kinase/protein kinase B dependent pathway. PMID: 26163884
    7. apelin is a potential activator of inflammation factors through the PI3K/Akt and Erk signaling pathway and is potential therapeutically relevant to inflammatory responses of microglia cells PMID: 25951933
    8. we showed the effect of Apelin on RAW264.7 macrophage under normal and hypoxic condition, which could further influence the angiogenesis and inflammation process that promoted by macrophages. PMID: 25963277
    9. PDL- and Cer-induced CP resulted in increased production of the pancreatic BMP2, apelin, and PTHrP signaling systems and that significant cross talk occurred among pancreatic BMP2, apelin, and PTHrP PMID: 26229008
    10. Therapeutic potential of apelin to prevent myocardial metabolic abnormalities in heart failure paired with obesity. PMID: 25027224
    11. Data suggest that CTRP3 (cartducin) up-regulates expression and secretion of adipokines (adiponectin, leptin, visfatin, and apelin) in adipocytes; these responses to CTRP3 are down-regulated by insulin resistance or inhibition of AMPK signaling. PMID: 25168658
    12. Apelin enhances not only the differentiation and metabolic activity of brown adipocytes but also the browning of white adipocytes. PMID: 25931124
    13. for the first time, our results indicate that apelin-13 blocks STM formation and LTM consolidation in novel object recognition task. PMID: 25445607
    14. Apelin-13 decreases lipid storage in hypertrophic adipocytes in vitro through the upregulation of AQP7 expression by the PI3K signaling pathway. PMID: 25080850
    15. Hypothalamic apelin is one master switch that participates in the onset of diabetes by directly acting on liver function. PMID: 23879244
    16. EPA increases apelin/APJ expression in muscle, apelin may act in a paracrine/autocrine manner to contribute to these benefical effects. PMID: 24244380
    17. APLN is a critical regulator of the myocardial response to infarction and ischemia. PMID: 23817469
    18. Data indicate that the protein expression levels of clock and apelin were significantly reduced when insulin-resistant adipocytes were treated with melatonin (Mel) and Neu-P11. PMID: 23963596
    19. ACE2 was downregulated in apelin-deficient mice. Apelin, via activation of its receptor, APJ, increased ACE2 promoter activity in vitro and upregulated ACE2 expression in failing hearts in vivo. ACE2 couples the RAS to the apelin system. PMID: 24177423
    20. Apelin is not only a potent endothelial growth factor, but also restricts pericyte recruitment, establishing a new connection between endothelial cell proliferation signaling and a trigger of mural recruitment. PMID: 23640575
    21. upregulated apelin in type 2 diabetes promotes angiogenesis in glomeruli to form abnormal vessels PMID: 23577111
    22. Apelin-APJ signaling plays a novel role as a potent regulator of endothelial MEF2 function in the developing cardiovascular system. PMID: 23603510
    23. Apelin ameliorates TNF-alpha-induced reduction of glycogen synthesis in the hepatocytes through G protein-coupled receptor APJ. PMID: 23437347
    24. The results indicate that apelin inhibits HFD-induced obesity by enhancing vessel integrity. Apelin could serve as a therapeutic target for treating obesity and related diseases. PMID: 23378608
    25. Apelin-13 elicits its action on the consolidation of passive avoidance learning via alpha-adrenergic, 5-HT2 serotonergic, cholinergic, dopaminergic, GABA-A-ergic and nitric oxide mediations. PMID: 23131543
    26. Results indicate that apelin functions as a physiologically significant antianabolic factor in bone in vivo. PMID: 23584856
    27. Apelin is able to inhibit lipolysis by stabilizing the lipid droplets. PMID: 22842084
    28. apelin/APJ system may be a crucial factor for pathological retinal angiogenesis.(review) PMID: 21804324
    29. a novel physiological role of apelin in alleviating endoplasmic reticulum stress in the pancreas of type 1 diabetes. PMID: 21762740
    30. apelin/APJ system in the spinal cord has a neuroprotective effect against the pathogenesis of amyotrophic lateral sclerosis PMID: 21887354
    31. Apelin and the proopiomelanocortin system: a new regulatory pathway of hypothalamic alpha-MSH release. PMID: 21846903
    32. These data provide compelling evidence that central apelin participates in the regulation of glucose homeostasis and suggest a novel pathophysiological mechanism involved in the transition from normal to diabetic state. PMID: 21395477
    33. Data describe a BMPR2-mediated transcriptional complex between PPARgamma and beta-catenin, and suggest that apelin could be effective in treating PAH by rescuing BMPR2 and PAEC dysfunction. PMID: 21821917
    34. Apelin negatively regulates lipolysis. Its actions may be mediated by pathways involving G(q), G(i), and AMP-activated protein kinase. PMID: 21047945
    35. apelin is a prerequisite factor for hypoxia-induced retinal angiogenesis PMID: 20705920
    36. Mechanisms mediating eicosaspentaeonic-acid induced apelin synthesis and/or secretion are complex, involving steps that are PI3K dependent and steps that are PI3K independent. PMID: 20352620
    37. apelin is necessary for the maintenance of insulin sensitivity in vivo PMID: 19861585
    38. apelin is an endogenous neuroprotective adipocytokine that may block apoptosis and excitotoxic death via cellular and molecular mechanisms PMID: 20152832
    39. Transgenic overexpression of apelin in keratinocytes induces enlarged but not leaky blood vessels in the dermis. PMID: 20185589
    40. Insulin and obesity up-regulate apelin in adipocytes. PMID: 15677759
    41. First evidence to our knowledge for apelin-mediated myosin light chain phosphorylation in vitro and in vivo, which is a potential mechanism of apelin-mediated vasoconstriction. PMID: 16556853
    42. Apelin might be a candidate to understand potential links between obesity and associated disorders such as inflammation and insulin resistance. PMID: 16723381
    43. These data suggest that apelin behaves as a potent activator of tumour neoangiogenesis by a paracrine effect on host vessels. PMID: 17563744
    44. Apelin is crucial to maintain cardiac contractility in pressure overload and aging PMID: 17673668
    45. novel evidence for the regulatory roles of renal apelin system in vascular functions in diabetes PMID: 17692936
    46. The apelin-APJ pathway may thus provide a mechanism for systemic endothelial monitoring of tissue perfusion and adaptive regulation of cardiovascular function. PMID: 17906101
    47. Spatiotemporally regulated apelin/APJ signaling participates in retinal vascularization in a cooperative manner with VEGF or FGF2, and contributes to normal ocular development. PMID: 18599802
    48. Hypoxia-inducible factor-1alpha knockdown abolishes hypoxia-induced apelin expression. PMID: 18617693
    49. apelin/APJ signaling pathways play a critical role in the development of the functional vascular network in adipose tissue. In addition, we have shown that adipocyte-derived apelin can be up-regulated by hypoxia. PMID: 18708591
    50. apelin signaling can block Angiotensin II actions in vascular disease by increasing NO production and inhibiting Angiotensin II cellular signaling PMID: 18769630

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  • 亚细胞定位：
    Secreted. Secreted, extracellular space.
  • 蛋白家族：
    Apelin family
  • 组织特异性：
    Expressed in extraembryonic visceral endoderm and in the primitive streak at 6.5 and 7.5 dpc. Expressed in the anterior visceral yolk sac at 8.25 dpc. Expressed weakly in the embryonic heart at 11.5 dpc. Expressed in the adult heart. Expressed in endothel
  • 数据库链接：

    KEGG: mmu:30878

    STRING: 10090.ENSMUSP00000046012

    UniGene: Mm.29262