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  4. Recombinant Mouse Cytosolic endo-beta-N-acetylglucosaminidase (Engase)

Recombinant Mouse Cytosolic endo-beta-N-acetylglucosaminidase (Engase)

  • 中文名称：
    小鼠Engase重组蛋白
  • 货号：
    CSB-YP805982MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Engase重组蛋白
  • 货号：
    CSB-EP805982MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Engase重组蛋白
  • 货号：
    CSB-EP805982MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Engase重组蛋白
  • 货号：
    CSB-BP805982MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Engase重组蛋白
  • 货号：
    CSB-MP805982MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Engase
  • Uniprot No.：
  • 别名：
    EngaseCytosolic endo-beta-N-acetylglucosaminidase; ENGase; EC 3.2.1.96
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-734
  • 氨基酸序列
    METSSVLTRG AARQRSPAAP EKQARDQTER RPGRRRQGRR INEDQEEEAV FREVVSFTPD PLPARYYDKD TTRPISFYLS TLEELLAWTP LMEDGFNVAL EPLVCRRPPL SSPRPRTLLC HDMMGGYLED RFIQGSEVQN PYSFYHWQYI DIFVYFSHHT VTIPPVCWTN AAHRHGVCVL GTFITEWQEG GRLCEAFLAG DEPSFQAVAD RLVQIAQFFR FDGWLINIEN SLTPAAVRNT PLFLQYLTAQ LHQQVPGGLV LWYDSVVQSG QLKWQDELND QNRVFFDSCD GFFTNYNWRE DHLQRMVAQA GERLADVYVG VDVFARSNVV GGRFDTDKSL ELIRKHGFSA ALFAPGWVYE CLEKSDFFQN QDKFWSLLER FLPTHSICSL PFVTSFCLGL GTRRVCYGKE QAVGPWYHPS AQETQPLFGE HKLAGDSRGW VKTHCCLTDA WHGGSSLLLR GLIPPEVDSV AVRLFSLHIP VPPKVFLSMV YKFEGSTDVQ VALELTTGDA SSCHVGGMLV LNETGSRHSP RPLRVPPTRL ARWASSCGQQ LSGGWIQRCY EVNLRGCLLQ DLLVSFSRPP GSREEESFIC RLGEIQVVDA SSLLAPLPRV QNVTISQIRW LPLITGSEGL PTRLLLSCTL HWSYLLLRAR CFRIHCWKRT GSSSSVAESP ETEKPTFLGL AFANQYRVVD LAVEAAGFGQ DGRVEFLVEP VPREGFLVPQ AEWGKAVLLF SVPQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.
  • 基因功能参考文献：
    1. These observations strongly suggest that the Ngly1- or Ngly1/Engase-deficient mice could serve as a valuable animal model for studies related to the pathogenesis of the NGLY1-deficiency, and that cytoplasmic ENGase represents one of the potential therapeutic targets for this genetic disorder. PMID: 28426790
    2. ENGase efficiently hydrolyzes phosphorylated oligosaccharides that are larger than Man4GlcNAc2-P, generating GlcNAc-1-P and neutral Gn1-type free oligosaccharides. PMID: 26858256
  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Glycosyl hydrolase 85 family
  • 数据库链接：

    KEGG: mmu:217364

    STRING: 10090.ENSMUSP00000117538

    UniGene: Mm.473963