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  4. Recombinant Mouse DNA (cytosine-5)-methyltransferase 3-like (Dnmt3l)

Recombinant Mouse DNA (cytosine-5)-methyltransferase 3-like (Dnmt3l)

  • 中文名称：
    小鼠Dnmt3l重组蛋白
  • 货号：
    CSB-YP863451MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Dnmt3l重组蛋白
  • 货号：
    CSB-EP863451MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Dnmt3l重组蛋白
  • 货号：
    CSB-EP863451MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Dnmt3l重组蛋白
  • 货号：
    CSB-BP863451MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Dnmt3l重组蛋白
  • 货号：
    CSB-MP863451MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dnmt3l
  • Uniprot No.：
  • 别名：
    Dnmt3lDNA; cytosine-5)-methyltransferase 3-like
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-421
  • 氨基酸序列
    MGSRETPSSC SKTLETLDLE TSDSSSPDAD SPLEEQWLKS SPALKEDSVD VVLEDCKEPL SPSSPPTGRE MIRYEVKVNR RSIEDICLCC GTLQVYTRHP LFEGGLCAPC KDKFLESLFL YDDDGHQSYC TICCSGGTLF ICESPDCTRC YCFECVDILV GPGTSERINA MACWVCFLCL PFSRSGLLQR RKRWRHQLKA FHDQEGAGPM EIYKTVSAWK RQPVRVLSLF RNIDKVLKSL GFLESGSGSG GGTLKYVEDV TNVVRRDVEK WGPFDLVYGS TQPLGSSCDR CPGWYMFQFH RILQYALPRQ ESQRPFFWIF MDNLLLTEDD QETTTRFLQT EAVTLQDVRG RDYQNAMRVW SNIPGLKSKH APLTPKEEEY LQAQVRSRSK LDAPKVDLLV KNCLLPLREY FKYFSQNSLP L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context. Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain. Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. Plays a key role in embryonic stem cells and germ cells. In germ cells, required for the methylation of imprinted loci together with DNMT3A. In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization. Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation. While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes. Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes. Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression.
  • 基因功能参考文献：
    1. The Sirt1 protein suppressed transcription of Dnmt3l. PMID: 28228259
    2. The Dnmt3l mutation greatly reduced DNA methylation levels at most retrotransposons, but its impact on their RNA abundance was limited in prospermatogonia. In Pld6 mutant germ cells, although only a few retrotransposons exhibited reduced DNA methylation, many showed increased expression at the RNA level. PMID: 28749988
    3. Dnmt3l-KO donor cells may offer a more permissive epigenetic state that is beneficial for Nuclear transfer reprogramming PMID: 26159833
    4. By interacting with TRIM28, DNMT3L can attract various enzymes to form a DNMT3L-induced repressive complex to remove active marks and add repressive marks to histone proteins. PMID: 24991018
    5. Hypomethylation of highly methylated CpG islands was caused by the downregulation of Dnmt3L and Dnmt3a due to hepatitis B X-protein bound to their promoters. PMID: 24941955
    6. DNMT3L is required to delicately balance the cycling and quiescence of spermatogonial progenitor cells PMID: 24850856
    7. Study demonstrates that Dnmt3L interacts with the Polycomb PRC2 complex in competition with the DNA methyltransferases Dnmt3a and Dnmt3b to maintain low methylation levels at the Histone 3 H3K27me3 regions. PMID: 24074865
    8. Data indicate that expression of Dnmt3a, Dnmt3b, Dnmt3L as well as maintenance Dnmt1o in oocytes and zygotes was not disrupted. PMID: 23866265
    9. Reduced expression of DNMT3L in male germ cells, associated with haploinsufficiency of the paternal-effect gene Dnmt3L, results in abnormal hypomethylation of prenatal germline progenitor cells. PMID: 23159436
    10. The maternal store of Dnmt3L is not involved in embryonic de novo methylation. PMID: 23293288
    11. Dnmt3l is one of the key players in de novo DNA methylation of imprinting control elements and retrotransposons, which occurs after genome-wide epigenetic erasure during germ cell development. (Review) PMID: 22671959
    12. We observe evidence for a context-dependent contribution of Dnmt3l to set and maintain CpG and non-CpG methylation at distinct classes of repetitive elements and selected single copy genes PMID: 22761581
    13. Data show in DNMT3L-deficient germ cells at 16.5 dpc, average DNA methylation levels were about 30% of wildtype levels; however, by postnatal day 6, about half of the DNMT3L deficiency-specific hypomethylated loci had acquired normal methylation levels. PMID: 21949694
    14. Reduced dosage of the modifiers of epigenetic reprogramming Dnmt1, Dnmt3L, SmcHD1 and Foxo3a has no detectable effect on mouse telomere length in vivo. PMID: 21553025
    15. show that both Dnmt3L(s) and Dnmt3L(o) produce full-length proteins but that the Dnmt3L(at) transcripts are not translated PMID: 21645502
    16. The data demonstrate previously unrecognised roles for DNMT3L in late meiosis and in the transcriptional regulation of meiotic and post-meiotic germ cells. PMID: 21483837
    17. CG dinucleotide recognized by the Dnmt3a and Dnmt3L complex are distinctive at retroelements and imprinted domains. PMID: 19921333
    18. likely to function not directly as a DNA methyltransferase but as a regulator of methylation at imprinted loci PMID: 11719692
    19. Dnmt3L is required for the establishment of methylation imprints in oocyte. Dnmt3L is required for spermatogenesis. PMID: 11934864
    20. Loss of Dnmt3L from early germ cells caused meiotic failure in spermatocytes, which do not express Dnmt3L PMID: 15318244
    21. Results suggest a model in which the interaction of Dnmt3A with Dnmt3L induces a conformational change of Dnmt3A that opens the active site of the enzyme and promotes binding of DNA and the AdoMet. PMID: 15671018
    22. The Dnmt3l genes showing marginal correlation (-.4 < p(s) < .6) were present in the early stages of differentiation of both GD and RD, which were non-specific to brain development. PMID: 16627687
    23. Dnmt3L is required for the establishment of maternal methylation imprints both in the embryos and the placentae PMID: 16920095
    24. Dnmt3a2 recruits Dnmt3L to chromatin, and induces regional DNA methylation in germ cells. PMID: 16999741
    25. Sex-specific promoters control Dnmt3L expression in the mouse germ line, mirroring the situation at the Dnmt1 and Dnmt3A loci. PMID: 17060371
    26. dnmt3l is coordinately regulated and its expression peaks during the stage of postnatal oocyte development when maternal methylation imprints are established PMID: 17445268
    27. Imprinted and repetitive sequences showed moderate to severe hypomethylation in Dnmt3L-deficient prospermatogonia, indicating the critical function and broad specificity of this factor in de novo methylation. PMID: 17616512
    28. The main proteins that interact in vivo with the product of an epitope-tagged allele of the endogenous Dnmt3L gene were identified by mass spectrometry as DNMT3A2, DNMT3B and the four core histones PMID: 17687327
    29. temporal expression study showed that expression of Dnmt3L is highest in prenatal gonocytes but is also detected and developmentally regulated during spermatogenesis PMID: 17875220
    30. Insufficient methylation of the DNMT3L promoter during embryonic development due to deficiency in DNMT3B might be implicated in the pathogenesis of the ICF syndrome (characterized by immunodeficiency, centromere instability and facial anomalies). PMID: 18544626

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in testis, thymus, ovary, and heart.
  • 数据库链接：

    KEGG: mmu:54427

    STRING: 10090.ENSMUSP00000000746

    UniGene: Mm.13433