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  4. Recombinant Mouse Double-strand-break repair protein rad21-like protein 1 (Rad21l1)

Recombinant Mouse Double-strand-break repair protein rad21-like protein 1 (Rad21l1)

  • 中文名称：
    小鼠Rad21l1重组蛋白
  • 货号：
    CSB-YP019258MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rad21l1重组蛋白
  • 货号：
    CSB-EP019258MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rad21l1重组蛋白
  • 货号：
    CSB-EP019258MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rad21l1重组蛋白
  • 货号：
    CSB-BP019258MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rad21l1重组蛋白
  • 货号：
    CSB-MP019258MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rad21l1
  • Uniprot No.：
  • 别名：
    Rad21l1; Gm14160; Rad21l; Double-strand-break repair protein rad21-like protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-552
  • 氨基酸序列
    MFYTHVLMSK RGPLAKIWLA AHWEKKLTKA HVFECNLEIT IQKIISPKVK IALRTSGHLL LGVVRIYNRK AKYLLADCSE AFLKMKMTFR PGLVDLPKEN FEAAYNTITL PEEFHDFEIY NINEIDISEP LAQNQSRPEE ITLREEYSND LLFQAGSFGD EPEILRRHSF FDDNILMNSS GLVVEHSSGS FAEEKSLFFD NGDGFGDEGA AGEMIDNLLQ DESTFLEEAY LNKEVSLPPE LPSSIMVEPG NSDDQCIPED EEINEITLLS NEDEGFTLDP IDDLDIADRR RRKKRRLLVD PVKEISSKAM HRQLASFMDT LMVLDLAPPT QRLMMWKKRG GVDMLLSTAT QDLINDELKM LFTKCFLSSD YKLAKLTLKE SVRKEVGNQQ IAEPSVMGEP NSHSELDQPQ DWKDVTDESV GSFQENVNMN VNSEQDILGM ISPAVEGLSS MNGSLAQENC PAELESSGSK QNTEAEKWNQ RLFQTLNVLR EFNKMGMQSF SLKKLCRNSD RKQAAAKFYT LLILKKHRAI ELSQSVPYAD IIATVGPMFY KM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Meiosis-specific component of some cohesin complex required during the initial steps of prophase I in male meiosis. Probably required during early meiosis in males for separation of sister chromatids and homologous chromosomes. Replaces RAD21 in premeiotic S phase (during early stages of prophase I), while RAD21 reappears in later stages of prophase I. Involved in synaptonemal complex assembly, synapsis initiation and crossover recombination between homologous chromosomes during prophase I. Not required for meiosis in females in young mice, while it is required later as mice age.
  • 基因功能参考文献：
    1. when ectopically expressed in somatic cells, can promote homologous chromosome adjacency, which resembles the homolog pairing normally seen during meiosis PMID: 28239026
    2. Our genetic analysis demonstrates that cohesins containing STAG3 and REC8 are the main complex required for centromeric cohesion, and RAD21L cohesins are required for normal clustering of pericentromeric heterochromatin. Furthermore, the STAG3/REC8 and STAG3/RAD21L cohesins are the primary cohesins required for axis formation. PMID: 27172213
    3. These results strongly support the previous proposition that RAD21L is an atypical cohesin that establishes the association between homologs rather than sister chromatids. PMID: 27665783
    4. Disruption of meiosis-specific cohesin RAD21L precludes the initial association of homologs as well as the subsequent pairing in spermatocytes PMID: 24589552
    5. RAD21L is identified as a novel subunit of the cohesin complex with homology to the RAD21/REC8/alpha-kleisin subfamily expressed in mouse testis. PMID: 21527826
    6. These results provide in vivo evidence that RAD21L is essential for male fertility and in females for the maintenance of fertility during natural aging. PMID: 21743440
    7. study identifes a new mammalian cohesin subunit, RAD21L, with sequence similarity to RAD21 and REC8; RAD21L localizes along axial elements in early meiotic prophase, in a manner that is spatiotemporally different to either REC8 or RAD21 PMID: 21274006
    8. The results suggest that cohesin complexes containing RAD21L may be involved in synapsis initiation and crossover recombination between homologous chromosomes. PMID: 21242291

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Rad21 family
  • 组织特异性：
    Specifically expressed in male and female gonads (at protein level).
  • 数据库链接：

    KEGG: mmu:668929

    STRING: 10090.ENSMUSP00000094174

    UniGene: Mm.473683