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  4. Recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 2 (Map2k2)

Recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 2 (Map2k2)

  • 货号：
    CSB-YP723614MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP723614MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP723614MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP723614MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP723614MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Map2k2; Mek2; Mkk2; Prkmk2; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; EC 2.7.12.2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-401
  • 氨基酸序列
    MLARRKPVLP ALTINPTIAE GPSPTSEGAS EANLVDLQKK LEELDLDEQQ RKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR KLIHLEIKPA VRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ GTHYSVQSDI WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP PGRPISVGHG MDSRPAMAIF ELLDYIVNEP PPKLPSGVFS SDFQEFVNKC LIKNPAERAD LKLLMNHAFI KRSEGEEVDF AGWLCRTLRL KQPSTPTRTA V
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.
  • 基因功能参考文献：
    1. MK2/3 cascade plays a strategic role in controlling synaptic plasticity and cognition. PMID: 25134715
    2. MK2 attenuates dendritic cell-mediated Th1 differentiation and autoimmune encephalomyelitis. PMID: 26078274
    3. analysis of p38-MK2-activated Rsk signaling in toll-like receptor-stimulated dendritic cells PMID: 25332232
    4. MK2 is responsible for resistance of p53-deficient tumors to cisplatin PMID: 24239348
    5. MK2 plays a critical role in the development of apoptosis. PMID: 24304496
    6. A critical role is indicated for p38 effector kinase MK2 in the regulation of tumor necrosis factor (TNF)-alpha messenger RNA (mRNA) translation through the TRIF signaling pathway. PMID: 20375303
    7. Pin1 regulates BPGAP1 function in Rho and Erk signalling, with active Mek2 serving as a novel regulatory scaffold that promotes crosstalk between RhoGAP, Pin1 and Erk in the regulation of cell migration. PMID: 20179103
    8. Results suggest a role for MK2 in the regulation of muscle mass, a role which, at least in part, may be related to determining the subcellular localization of p38 in muscle fibers. PMID: 20049871
    9. MK2, in contrast to its downstream effector molecule, TNF-alpha, has a rather elusive role in T-cell-dependent cutaneous inflammation PMID: 19657354
    10. MK2 signaling has a minor role in skin inflammation [commentary] PMID: 20081887
    11. MK2 stabilizes p38 MAPK through its C terminus and that MK2 catalytic activity does not contribute to this stabilization. PMID: 12052889
    12. MK2 has a role in cytokine expression and in cell migration [review] PMID: 12440954
    13. our findings demonstrate that MEK2 is not necessary for the normal development of the embryo and T-cell lineages, suggesting that the loss of MEK2 can be compensated for by MEK1. PMID: 12832465
    14. MK2 inhibits the mRNA destabilizing activity of tristetraprolin and, in parallel, codegradation of tristetraprolin together, with the target mRNA resulting in increased cellular levels of tristetraprolin. PMID: 16508014
    15. MK2-dependent insolubilization of Hsp25 oligomers has a role in susceptibility to cell stress PMID: 16840785
    16. Fyn-calcineurin-NFATc2 and MEK2-ERK1/2 are two independent signaling pathways that cooperate to control T cell receptor-mediated ICOS induction PMID: 16880206
    17. These results indicate a critical role of p38-MK2-dependent induction of MKP-1 in innate PMID: 16978838
    18. MK2 mediates the incorporation of p38 into the pre-existing complex of hsp27 with Akt PMID: 17015449
    19. MK2-HSP27 pathway is a target for therapeutic control of localized inflammatory diseases. PMID: 17576778
    20. MK2 critically promotes atherogenesis by fostering foam cell formation and recruitment of monocytes/macrophages into the vessel wall. PMID: 17885219
    21. p38 MAP kinase and its downstream effector MK2 mediate lung permeability in ventilator associated lung injury by regulating HSP25 phosphorylation and actin cytoskeletal remodeling. PMID: 19240800
    22. MK2 deficient mouse macrophages produce markedly decreased levels TNF-alpha than wild type macrophages. PMID: 19359247
    23. MK2 has a role in the early stages of tumor promotion through regulation of both the inflammatory response and apoptosis of DNA-damaged cells PMID: 19808857

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  • 亚细胞定位：
    Cytoplasm. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
  • 组织特异性：
    Expressed in adult intestine, kidney, liver, lung, pancreas, spleen, thymus, and at high levels in the neonatal brain. Lower expression is found in adult brain and heart.
  • 数据库链接：

    KEGG: mmu:26396

    STRING: 10090.ENSMUSP00000121111

    UniGene: Mm.275436