Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse E3 SUMO-protein ligase EGR2 (Egr2)

Recombinant Mouse E3 SUMO-protein ligase EGR2 (Egr2)

  • 中文名称：
    小鼠Egr2重组蛋白
  • 货号：
    CSB-YP007485MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Egr2重组蛋白
  • 货号：
    CSB-EP007485MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Egr2重组蛋白
  • 货号：
    CSB-EP007485MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Egr2重组蛋白
  • 货号：
    CSB-BP007485MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Egr2重组蛋白
  • 货号：
    CSB-MP007485MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Egr2; Egr-2; Krox-20; Zfp-25E3 SUMO-protein ligase EGR2; EC 2.3.2.-; E3 SUMO-protein transferase ERG2; Early growth response protein 2; EGR-2; Zinc finger protein Krox-20
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-470
  • 氨基酸序列
    MMTAKAVDKI PVTLSGFMHQ LPDSLYPVED LAASSVTIFP NGELGGPFDQ MNGVAGDGMI NIDMTGEKRP LDLPYPSSFA PISAPRNQTF TYMGKFSIDP QYPGASCYPE GIINIVSAGI LQGVTPPAST TASSSVTSAS PNPLATGPLG VCTMSQTQPE LDHLYSPPPP PPPYSGCTGD LYQDPSAFLS PPSTTSTSSL AYQPPPSYPS PKPAMDPGLI PMIPDYPGFF PSPCQRDPHG AAGPDRKPFP CPLDSLRVPP PLTPLSTIRN FTLGGPGAGV TGPGASGGGE GPRLPGSGSA AVTATPYNPH HLPLRPILRP RKYPNRPSKT PVHERPYPCP AEGCDRRFSR SDELTRHIRI HTGHKPFQCR ICMRNFSRSD HLTTHIRTHT GEKPFACDYC GRKFARSDER KRHTKIHLRQ KERKSSAPSA PPSAQSSASG PGGSQAGGSL CGNSAIGGPL ASCTSRTRTP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sequence-specific DNA-binding transcription factor. Plays a role in hindbrain segmentation by regulating the expression of a subset of homeobox containing genes and in Schwann cell myelination by regulating the expression of genes involved in the formation and maintenance of myelin. Binds to two EGR2-consensus sites EGR2A (5'-CTGTAGGAG-3') and EGR2B (5'-ATGTAGGTG-3') in the HOXB3 enhancer and promotes HOXB3 transcriptional activation. Binds to specific DNA sites located in the promoter region of HOXA4, HOXB2 and ERBB2. Regulates hindbrain segmentation by controlling the expression of Hox genes, such as HOXA4, HOXB3 and HOXB2, and thereby specifying odd and even rhombomeres. Promotes the expression of HOXB3 in the rhombomere r5 and of HOXB3 in r3 and r5 in the hindbrain. Regulates myelination in the peripheral nervous system after birth, possibly by regulating the expression of myelin proteins, such as MPZ, and by promoting the differentiation of Schwann cells. Involved in the development of the jaw openener musculature, probably by playing a role in its innervation through trigeminal motor neurons. May play a role in adipogenesis, possibly by regulating the expression of CEBPB.; E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity.
  • 基因功能参考文献：
    1. Study shows the conversion of mouse skin fibroblasts into induced Schwann cells by driving the expression of two transcription factors, Sox10 and Egr2. PMID: 28169300
    2. Egr2 and Egr3 expressed in T cells cooperatively prevent humoral immune responses by supporting TGF-beta3 secretion. PMID: 27911796
    3. study revealed GCN5-mediated EGR2 acetylation as a molecular mechanism that regulates iNKT development. PMID: 28723564
    4. findings demonstrate a crucial role for Krox20 in arterial valve development and reveal that an excess of neural crest cells may be associated with bicuspid aortic valve. PMID: 29158447
    5. these results suggested that the basal promoter activity of the mIRF-3 gene is regulated by transcription factors Egr2 and YY1 in NIH3T3 cells PMID: 26740329
    6. Prss56 expression was regulated by Egr2 during mouse decidualization. PMID: 28611210
    7. Egr2 and 3 are upstream regulators of effector CD4 and CD8 T cells that are essential for optimal responses with limited immunopathology. PMID: 28487311
    8. Egr2 and 3 are antagonists of T-bet function in effector T cells and are important for the control of inflammatory responses of T cells. PMID: 28455436
    9. We discovered that Tead1 and co-activators Yap and Taz are required for Pmp22 expression, as well as for the expression of Egr2 Tead1 directly binds Pmp22 and Egr2 enhancers early in development and Tead1 binding is induced during myelination, correlating with Pmp22 expression. The data identify Tead1 as a novel regulator of Pmp22 expression during development in concert with Sox10 and Egr2 PMID: 27288457
    10. endogenous KLF4 and Krox20 are dispensable for adipogenesis in culture and for brown adipose tissue development in mice. In contrast, the master adipogenic transcription factor peroxisome proliferator-activated receptor gamma (PPARgamma) is essential. PMID: 27777310
    11. Decreased expression of Krox20 in mice causes degeneration of the aortic leaflets and disorganization of the extracellular matrix, causing valvular dysfunction. PMID: 26711547
    12. Mir106b regulates pro-allergic properties of dendritic cells and Th2 polarisation by targeting Egr-2 in vitro PMID: 25864617
    13. let-7 miRNAs promote expression of the myelination-driving master transcription factor Krox20 PMID: 26466203
    14. The data suggest that Pak2 controls thymic Natural Killer T-cell development by providing a signal that links Egr2 to induce PLZF, in part by regulating signaling lymphocyte activation molecule 6 expression. PMID: 26519537
    15. demonstrate a novel function of EGR2/3 that is important for Tfh cell development and Tfh cell-mediated B cell immune responses PMID: 25979336
    16. Egr-2 was upregulated by NF-kappaB activation in p50+/+ hippocampal slices. PMID: 25553923
    17. EGR2 promotes peripheral naive T-cell differentiation, with delayed T-cell receptor-induced proliferation in naive T cells from Egr2 conditional knockout mice, and decreased production of cytokines in cells subjected to T-helper differentiation. PMID: 25368162
    18. A direct, positive autoregulatory loop amplifies and maintains the expression of Krox20, a transcription factor governing vertebrate hindbrain segmentation. PMID: 24061538
    19. The results indicated a novel role for Egr2 in repressing adipocyte lineage commitment and promoting early smooth muscle-like cell differentiation. PMID: 23751188
    20. These findings support a model in which Egr2-neurons are a critical component of the respiratory chemoreflex into adulthood. PMID: 23261662
    21. Egr2 acts as an intrinsic negative regulator of dendritic cell immunogenicity and can be an attractive molecular target for dendritic cell vaccine development. PMID: 23716134
    22. Data indicate that epidermal growth factor receptor (EGFR) signaling stimulates the expression of transcription factor early growth response 2 (EGR2) to promote osteoprogenitor proliferation and survival. PMID: 23720781
    23. Mast cell Egr2 was essential for migration of CD4(+) T cells to the inflamed lung. PMID: 23536637
    24. ChIP-Seq analysis identified genes that bind Egr2 in anergic T cells, revealing 49 targets that are directly regulated by Egr2. PMID: 23548837
    25. Egr-2 induced during T helper (Th)17 cell differentiation serves as a negative feedback inhibitor to control Th17-mediated inflammation. PMID: 23203924
    26. results identify Egr2 as an important modulator of RANKL-induced osteoclast differentiation and provide the link between RANKL, Egr2 and Id proteins in osteoclast-lineage cells PMID: 22842221
    27. Transcriptional regulator early growth response gene 2 (Egr2) is required for T cell anergy in vitro and in vivo. PMID: 23129747
    28. discovered that Egr2 and/or Egr3 directly induced expression of suppressor of cytokine signaling-1 (SOCS1) and SOCS3, inhibitors of STAT1 and STAT3, and also blocked the function of Batf, an AP-1 inhibitor, in B and T cells PMID: 23021953
    29. Loading-related regulation of transcription factor EGR2/Krox-20 in bone cells is ERK1/2 protein-mediated and prostaglandin, Wnt signaling pathway-, and insulin-like growth factor-I axis-dependent. PMID: 22049075
    30. results demonstrate that Egr-2 expressed in restricted stages of lymphocyte development plays a dynamic, but similar role for the development of T, NKT and B cells PMID: 21533228
    31. these findings suggest that Egr-2 plays an important nonredundant role in the pathogenesis of fibrosis. PMID: 21514423
    32. Egr2 and Sox10 activity are directly involved in mediating the developmental induction of Pmp22 expression through an intronic enhancer. PMID: 21411665
    33. Inactivation of Krox20/Egr2, a master regulatory gene for myelination in Schwann cells, results in transgression of the CNS/PNS boundary by astrocytes and oligodendrocytes and in myelination of nerve root axons by oligodendrocytes. Review. PMID: 21193208
    34. EGR-2 is not required for CD4 T cell mediated immune responses. PMID: 20886122
    35. the antimitogenic role of Krox20 in preosteoclasts is the predominant mechanism underlying the low bone mass phenotype of Krox20-deficient mice PMID: 20716776
    36. data suggest that maintenance of the CNS/PNS boundary requires a Krox20 function separable from myelination control PMID: 20427655
    37. survival function of Egr2 at least partly depends upon its ability to activate the cytokine-mediated survival pathway. PMID: 19877014
    38. These results suggest that the dependency of the enhancer on both crest- (Sox10) and r5- (Krox20) specific factors limits its activity to the r5-derived neural crest. PMID: 12538520
    39. Krox-20 protein has a role in the maturation of particular brainstem and cerebellar neurons and fluctuations in Krox-20 protein expression coincide with the development of circuitry underlying brainstem-mediated behaviors PMID: 12706208
    40. that the role of Krox-20 is to amplify an earlier Krox-20-independent activation of the periaxin gene in Schwann cells PMID: 12799134
    41. Ddx20 repressed Egr2-mediated transcriptional activation with significant promoter specificity PMID: 14699164
    42. Data suggest that Krox-20 can control suppression of mitogenic and death responses, up-regulate the scaffold protein c-Jun NH2-terminal protein kinase (JNK)-interacting protein 1, and regulate JNK activity during Schwann cell development. PMID: 14757751
    43. Egr-2 and Egr-3 transcription is enhanced by Hepatitis B virus X protein, which induces fasL gene expression PMID: 15173177
    44. Sustained Egr-2 expression is necessary to induce a full anergic state through the actions of genes regulated by transcription factor Egr-2. PMID: 15585857
    45. Because glucocorticoids inhibit Egr2/Krox20 expression in osteoblasts and trabecular bone formation is arrested in Egr2/Krox20-knockout mice, inhibition of Egr2/Krox20 activity likely contributes to glucocorticoid-induced osteoporosis. PMID: 15751078
    46. Egr-2 and Egr-3 are involved in promoting a T cell receptor-induced negative regulatory genetic program PMID: 15834410
    47. Direct induction of cholesterol/lipid biosynthetic genes by Egr2/Krox20 is a part of the myelination program regulated by the sterol regulatory element binding protein (SREBP) pathway. PMID: 15836632
    48. Krox-20 regulates the period and extent of Me5 apoptosis, impacting the final number of Me5 neurons. The loss of Me5 in Krox-20-/- mice may highlight species-specific differences in the origin of these cells. PMID: 15886484
    49. analysis of an Egr-2-directed transcriptional regulatory network controlled by striatal A2ARs PMID: 16046619
    50. Results suggest that cooperative interactions between Egr2 and Sox10 mediate a large increase in myelin protein zero expression to the high levels found in myelinating Schwann cells. PMID: 16373334

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    EGR C2H2-type zinc-finger protein family
  • 组织特异性：
    Expressed mainly in the thymus.
  • 数据库链接：

    KEGG: mmu:13654

    STRING: 10090.ENSMUSP00000041053

    UniGene: Mm.290421