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  4. Recombinant Mouse Eukaryotic translation initiation factor 6 (Eif6)

Recombinant Mouse Eukaryotic translation initiation factor 6 (Eif6)

  • 中文名称：
    小鼠Eif6重组蛋白
  • 货号：
    CSB-YP007582MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Eif6重组蛋白
  • 货号：
    CSB-EP007582MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Eif6重组蛋白
  • 货号：
    CSB-EP007582MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Eif6重组蛋白
  • 货号：
    CSB-BP007582MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Eif6重组蛋白
  • 货号：
    CSB-MP007582MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Eif6
  • Uniprot No.：
  • 别名：
    Eif6; Itgb4bpEukaryotic translation initiation factor 6; eIF-6; B4 integrin interactor; CAB; p27(BBP)
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-245
  • 氨基酸序列
    MAVRASFENN CEVGCFAKLT NAYCLVAIGG SENFYSVFEG ELSDAIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLPD SVQIRRVEER LSALGNVTTC NDYVALVHPD LDRETEEILA DVLKVEVFRQ TVADQVLVGS YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP LVAGTVNRGS EVIAAGMVVN DWCAFCGLDT TSTELSVVES VFKLNEAKPS TIATSMRDSL IDSLT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth.
  • 基因功能参考文献：
    1. eIF6 heterozygous mice show an increased mortality during viral infection and a reduction of peripheral blood CD4(+) effector memory T cells PMID: 28743432
    2. Taken together, these results indicate that eIF6 may be involved in external mechanical force-mediated murine dermal fibroblast function at least partly through the TGF-beta1/TGFBR1/TGFBR2 pathway. PMID: 27824055
    3. eIF6 relieved fibrosis/cicatrization by inhibiting the generation of VEGF to prevent the overgrowth of blood vessels and production of granulation tissues, and by regulating the MMP-2/TIMP-2 balance to promote ECM degradation and decrease its deposition. PMID: 27220600
    4. data reveal a novel transcriptional regulatory mechanism of eIF6 that acts on facilitating Sp1 recruitment to TGF-beta1 promoter via H2A.Z depletion and thus results in increased TGF-beta1 transcription, which contributes to myofibroblast differentiation. PMID: 26395397
    5. Reduced eIF6 expression negatively impacts on megakaryopoiesis. in eIF6(+/-) cells, the steady-state abundance of mitochondrial respiratory chain complex I-encoding mRNAs is decreased, resulting in decreased reactive oxygen species production. PMID: 26391622
    6. eIF6 controls fatty acid synthesis and glycolysis in a cell autonomous fashion. Translational activation by eIF6 reshapes gene expression with increased levels of lipogenic and glycolytic enzymes. PMID: 26383020
    7. In a murine model of lymphomagenesis, eIF6 depletion leads to a striking increase of survival, without adverse effects. PMID: 25252159
    8. eIF6 is a rate-limiting controller of initiation of translation, able to affect tumorigenesis and tumor growth. PMID: 21665150
    9. role in forming nuclear matrix filaments PMID: 12150946
    10. eIF6 is the first eIF associated with the large 60S subunit that regulates translation in response to extracellular signals PMID: 18784653

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  • 亚细胞定位：
    Cytoplasm. Nucleus, nucleolus. Note=Shuttles between cytoplasm and nucleus/nucleolus.
  • 蛋白家族：
    EIF-6 family
  • 组织特异性：
    Detected in bladder, duodenum, liver, esophagus, pancreas, adipose tissue, megakaryocytes and testis with lower levels in muscle (at protein level).
  • 数据库链接：

    KEGG: mmu:16418

    STRING: 10090.ENSMUSP00000029142

    UniGene: Mm.271674