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  4. Recombinant Mouse Hairy and enhancer of split-related protein HELT (Helt)

Recombinant Mouse Hairy and enhancer of split-related protein HELT (Helt)

  • 中文名称：
    小鼠Helt重组蛋白
  • 货号：
    CSB-YP766516MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Helt重组蛋白
  • 货号：
    CSB-EP766516MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Helt重组蛋白
  • 货号：
    CSB-EP766516MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Helt重组蛋白
  • 货号：
    CSB-BP766516MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Helt重组蛋白
  • 货号：
    CSB-MP766516MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Helt
  • Uniprot No.：
  • 别名：
    Helt; Hesl; Mgn; Hairy and enhancer of split-related protein HELT; HES/HEY-like transcription factor; Protein Hes-like; Protein megane
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-240
  • 氨基酸序列
    MSDRLKERKR TPVSHKVIEK RRRDRINRCL NELGKTVPMA LAKQSSGKLE KAEILEMTVQ YLRALHSADF PRGREKELLA EFANYFHYGY HECMKNLVHY LTTVERMETK DTKYARILAF LQSKARLGAE PTFPPLSLPE PDFSYQLHAA SPEFPGHSPG EATMFPQGAT PGSFPWPPGA ARSPALPYLS SATVPLPSPA QQHSPFLAPM QGLDRHYLNL IGHGHPNGLN LHTPQHPPVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'. Required for the development of GABAergic neurons.
  • 基因功能参考文献：
    1. the combination of gain- and loss-of-function experiments in the developing midbrain showed co-operative roles for Mgn and Mash1 genes in determining GABAergic identity. PMID: 25993409
    2. the prethalamic factors Dlxs upregulated by Ascl1/Helt deficiency play unique roles in regulating thalamic progenitor specification. PMID: 25512300
    3. Helt acts as a transcriptional repressor to regulate neuronal differentiation and/or identity PMID: 14764602
    4. identification of a novel mouse bHLH factor, termed Heslike, that has Hes1-like bHLH domain and transcriptional repressor activity; Heslike is coexpressed with the bHLH factor Mash1 in brain regions that give rise to GABAergic neurons PMID: 15071116
    5. Mgn is a single copy gene on mouse chromosome 8 and encodes a 27kDa protein that functions in the nucleus. PMID: 16644143
    6. Homozygous Mgn-null mice die between the second and the fifth postnatal week of age, and show a complete depletion of Gad65 and Gad67 expression in the superior colliculus and a reduction in the inferior colliculus. PMID: 16968817
    7. Helt determines GABAergic over glutamatergic fate, by repressing Ngn genes; Helt and Ngns are commonly used in the mesencephalon for determination of the GABAergic versus glutamatergic transmitter phenotype. PMID: 17611227

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    HEY family
  • 组织特异性：
    Expressed in heart and testis.
  • 数据库链接：

    KEGG: mmu:234219

    STRING: 10090.ENSMUSP00000054823

    UniGene: Mm.145631