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  4. Recombinant Mouse Immunity-related GTPase family M protein 1 (Irgm1)

Recombinant Mouse Immunity-related GTPase family M protein 1 (Irgm1)

  • 中文名称：
    小鼠Irgm1重组蛋白
  • 货号：
    CSB-YP730655MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Irgm1重组蛋白
  • 货号：
    CSB-EP730655MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Irgm1重组蛋白
  • 货号：
    CSB-EP730655MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Irgm1重组蛋白
  • 货号：
    CSB-BP730655MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Irgm1重组蛋白
  • 货号：
    CSB-MP730655MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Irgm1
  • Uniprot No.：
  • 别名：
    Irgm1; Ifi1; Iigp3; IrgmImmunity-related GTPase family M protein 1; EC 3.6.5.-; Interferon-inducible GTPase 3; Interferon-inducible protein 1; LPS-stimulated RAW 264.7 macrophage protein 47; LRG-47
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-409
  • 氨基酸序列
    MKPSHSSCEA APLLPNMAET HYAPLSSAFP FVTSYQTGSS RLPEVSRSTE RALREGKLLE LVYGIKETVA TLSQIPVSIF VTGDSGNGMS SFINALRVIG HDEDASAPTG VVRTTKTRTE YSSSHFPNVV LWDLPGLGAT AQTVEDYVEE MKFSTCDLFI IIASEQFSSN HVKLSKIIQS MGKRFYIVWT KLDRDLSTSV LSEVRLLQNI QENIRENLQK EKVKYPPVFL VSSLDPLLYD FPKLRDTLHK DLSNIRCCEP LKTLYGTYEK IVGDKVAVWK QRIANESLKN SLGVRDDDNM GECLKVYRLI FGVDDESVQQ VAQSMGTVVM EYKDNMKSQN FYTLRREDWK LRLMTCAIVN AFFRLLRFLP CVCCCLRRLR HKRMLFLVAQ DTKNILEKIL RDSIFPPQI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Putative GTPase which is required for IFNG-mediated clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. Required for macrophage motility and possibly also for adhesion.
  • 基因功能参考文献：
    1. This work establishes the importance of Irgm1(-/-) mice as a model to elucidate host-environment interactions that regulate mucosal homeostasis and intestinal inflammatory responses. PMID: 29361512
    2. Irgm1/IRGM participates in the polarization of M1 macrophages, is upregulated in human atheromata, and promotes development of atheroma in murine experimental atherosclerosis PMID: 27439214
    3. M1 macrophage may promote Th1/Th17 cell differentiation during the early experimental autoimmune encephalomyelitis, and the proinflammatory function of M1 cells requires Irgm1. PMID: 27443879
    4. A series of metabolic studies indicated that the enhanced cytokine production was associated with marked metabolic changes in the Irgm1-deficient macrophages, including increased glycolysis and an accumulation of long chain acylcarnitines. PMID: 28154172
    5. data suggested that the deficiency of LRG-47 might affect host's CD4(+) T cell immune response via the weakening of IFN-gamma downstream signaling PMID: 26660918
    6. Irgm1-/- embryonic fibroblasts show autophagic flux impairment and defective lysosomal degradation. PMID: 27098192
    7. Interestingly, we observed that IRGM1 enhanced F-actin polymerization and triggers epithelial mesenchymal transition (EMT) through a mechanism involved in PIK3CA mediated Rac1 activation. PMID: 26202910
    8. Interferon-beta activation recruited the immunity-related GTPase M1 (IRGM1) to the Toxoplasma gondii-occupied parasitophorous vacuole in peritoneal macrophages and murine embryonic fibroblasts. PMID: 25628099
    9. these data reveal an Irgm1-dependent mechanism that promotes the tumorigenesis of melanoma via dual regulation of apoptosis and Bif-1-dependent autophagy PMID: 25619828
    10. Palmitoylation together with the alphaK helix firmly anchor Irgm1 in the Golgi and mitochondria, thus facilitating function of the protein. PMID: 24751652
    11. 2 Irgm1 isoforms generated by differential splicing localize to the Golgi apparatus & weakly to mitochondria. Only the long isoforms is seen on endolysosomal membranes. Irgm1 is a negative regulator of the immunity-related GTPase protein family. PMID: 23842753
    12. the paralogous IRGM proteins Irgm1 and Irgm3 fail to robustly associate with "non-self" PVs containing either the bacterial pathogen Chlamydia trachomatis or the protozoan pathogen Toxoplasma gondii PMID: 23785284
    13. By 9 weeks post-infection, fewer Brucella abortus were recovered from LRG-47(-/-) mice than controls, suggesting that the host protein has a positive role in maintaining long term persistence of the bacteria within the host. PMID: 22919646
    14. These results suggest that Irgm1 modulates acute inflammatory responses in the mouse intestine, putatively through the regulation of gut autophagic processes, that may be pivotal for proper Paneth cell functioning. PMID: 23989005
    15. Loss of IRGM1 is associated with impaired CD36 internalization in macrophage, which was associated with the deficiency of F-actin polymerization in atherosclerosis. PMID: 23689639
    16. These data demonstrate a novel role of IRGM1 in regulating neuronal autophagy and survival during ischemic stroke. PMID: 22874556
    17. These data suggest that Irgm1 is a proinflammatory factor for Experimental autoimmune encephalomyelitis PMID: 22796503
    18. IFN-gamma enhances Con A-induced autophagic flux and causes an IRGM1-dependent lysosome-mediated necrotic cell death in hepatocytes PMID: 22163006
    19. Data show that hyperproliferation, self-renewal, and autophagy defects in Irgm1(-/-) HSCs were normalized in double-knockout Irgm1(-/-)Ifngr1(-/-) and Irgm1(-/-)Stat1(-/-), and the defects were abolished in Irgm1(-/-)Irgm3(-/-) double-knockout. PMID: 21633090
    20. Irgm1/LRG-47 is a significant factor in the progression of experimental autoimmune encephalomyelitis and multiple sclerosis. PMID: 20056715
    21. tagging of Irgm1 leads to loss of Golgi localisation and enhanced localisation on endolysosomal membranes, probably as a result of constitutive activation PMID: 20072621
    22. Irgm1 likely controls macrophage motility by regulating the positioning of specific GKS IRG proteins to the plasma membrane, which in turn, modulate cytoskeletal remodeling PMID: 19920210
    23. LRG-47 may serve as a critical vacuolar trafficking component used to dispose of intracellular pathogens like Mycobacterium tuberculosis PMID: 14576437
    24. A role is established for LRG-47 in host control of mycobacteria; in the context of the IFN-gamma response to persistent infection, LRG-47 can have downstream regulatory effects on lymphocyte survival. PMID: 14707092
    25. LRG-47 is a Golgi-associated protein in the IFN-stimulated cell, which is rapidly recruited to active plasma membrane upon phagocytosis and remains associated with phagosomes as they mature PMID: 15294976
    26. LRG-47 can influence pathogen control by simultaneously regulating macrophage-microbicidal activity and hemopoietic function PMID: 16339555
    27. Irgm1 (LRG-47)induced autophagy and generated large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium var. bovis BCG PMID: 16888103
    28. p47 GTPase LRG47 is involved in resistance to Trypanosoma cruzi infection in dendritic cells and macrophages. PMID: 17082622
    29. In lipopolysaccharide-stimulated murine macrophages LRG47 is induced by IFN-beta and negatively regulates Toll-like receptor 4 signaling to prevent excess proinflammatory cytokine production and shock. PMID: 17911638
    30. Irgm1 plays a key role in regulating the adhesion and motility of activated macrophages. PMID: 17982087
    31. inhibits baseline hematopoietic stem cells (HSC) proliferation and is required for a normal HSC response to chemical and infectious stimuli PMID: 18371424
    32. Irgm1 is essential for the expansion of CD4+ T lymphocyte populations after TCR stimulation. PMID: 18806793
    33. Irgm1 plays a major role in maintaining T lymphocyte homeostasis during host IFN gamma responses by protecting these cells from autophagy-dependent cell death. PMID: 19066452
    34. involved in interferon-gamma dependent control of M. tuberculosis infection in mouse model of tuberculosis PMID: 19120485
    35. Irgm3 functions to counter the effects of Irgm1. PMID: 19176402
    36. Cooperation between Irgm1 and PI(3)K further facilitated the engagement of Irgm1 with its fusogenic effectors at the site of infection, thereby ensuring pathogen-directed responses during innate immunity. PMID: 19620982

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  • 亚细胞定位：
    Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle, phagosome membrane. Cytoplasmic vesicle, autophagosome membrane. Lysosome membrane. Late endosome membrane. Cell projection, phagocytic cup. Note=Behaves like an integral membrane protein. Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing phagosomes. Association with phagosomes is dependent on nucleotide-binding but is IFNG-independent. Preferentially associated with cis- and medial-Golgi. Also detected in late endosomes and lysosomes.
  • 蛋白家族：
    TRAFAC class dynamin-like GTPase superfamily, IRG family
  • 组织特异性：
    Expressed in lung and primary macrophages.
  • 数据库链接：

    KEGG: mmu:15944

    STRING: 10090.ENSMUSP00000050446

    UniGene: Mm.29938