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  4. Recombinant Mouse Integrin beta-1-binding protein 1 (Itgb1bp1)

Recombinant Mouse Integrin beta-1-binding protein 1 (Itgb1bp1)

  • 中文名称：
    小鼠Itgb1bp1重组蛋白
  • 货号：
    CSB-YP011881MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Itgb1bp1重组蛋白
  • 货号：
    CSB-EP011881MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Itgb1bp1重组蛋白
  • 货号：
    CSB-EP011881MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Itgb1bp1重组蛋白
  • 货号：
    CSB-BP011881MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Itgb1bp1重组蛋白
  • 货号：
    CSB-MP011881MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Itgb1bp1
  • Uniprot No.：
  • 别名：
    Itgb1bp1Integrin beta-1-binding protein 1; Bodenin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-200
  • 氨基酸序列
    MFRKGKKRHS SSSSQSSEIS TKSKSVDSSL GGLSRSSTVA SLDTDSTKSS GQSNSNLDTC AEFRIKYVGA IEKLAVSEGK SLEGPLDLIN YIDVAQQDGK LPFVPLEEEF ILGVSKYGIK VSTTDQHGVL HRHALYLIIR MVCYDDGLGA GKSLLALKTT DASNEEYSLW VYQCNSLEQA QAICKVLSTA FDSVLTSDKS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail and preventing the activation of integrin alpha-5/beta-1 (heterodimer of ITGA5 and ITGB1) by talin or FERMT1. Plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. Stimulates cellular proliferation in a fibronectin-dependent manner. Involved in the regulation of beta-1 integrin-containing focal adhesion (FA) site dynamics by controlling its assembly rate during cell adhesion; inhibits beta-1 integrin clustering within FA by directly competing with talin TLN1, and hence stimulates osteoblast spreading and migration in a fibronectin- and/or collagen-dependent manner. Acts as a guanine nucleotide dissociation inhibitor (GDI) by regulating Rho family GTPases during integrin-mediated cell matrix adhesion; reduces the level of active GTP-bound form of both CDC42 and RAC1 GTPases upon cell adhesion to fibronectin. Stimulates the release of active CDC42 from the membranes to maintain it in an inactive cytoplasmic pool. Participates in the translocation of the Rho-associated protein kinase ROCK1 to membrane ruffles at cell leading edges of the cell membrane, leading to an increase of myoblast cell migration on laminin. Plays a role in bone mineralization at a late stage of osteoblast differentiation; modulates the dynamic formation of focal adhesions into fibrillar adhesions, which are adhesive structures responsible for fibronectin deposition and fibrillogenesis. Plays a role in blood vessel development; acts as a negative regulator of angiogenesis by attenuating endothelial cell proliferation and migration, lumen formation and sprouting angiogenesis by promoting AKT phosphorylation and inhibiting ERK1/2 phosphorylation through activation of the Notch signaling pathway. Promotes transcriptional activity of the MYC promoter.
  • 基因功能参考文献：
    1. ICAP-1 monoubiquitylation helps in switching from ROCK2-mediated to MRCKalpha-mediated cell contractility. PMID: 28049720
    2. The CCM1 loss resulted in ICAP-1 destabilization, which increased beta1 integrin activation and led to increased RhoA-dependent contractility. PMID: 23918940
    3. The ICAP-1 works in concert with kindlin-2 to control the dynamics of beta1 integrin-containing fibrillar adhesions and, thereby, regulates fibronectin deposition and osteoblast mineralization. PMID: 21768292
    4. ICAP-1 regulates beta1 integrin-dependent cell migration by affecting the pattern of focal adhesion formation. PMID: 17654484
    5. Identify ICAP1 as a novel regulator to prevent excessive sprouting angiogenesis. PMID: 20616313
    6. Integrin cytoplasmic domain-associated protein-1 (ICAP-1) binds ROCK to beta1 integrin at the cell membrane (ICAP-1) PMID: 16741948
    7. ICAP-1 promotes differentiation of osteoprogenitors by supporting their condensation through modulating the integrin high affinity state. PMID: 17567669

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle.
  • 组织特异性：
    Expressed in the brain.
  • 数据库链接：

    KEGG: mmu:16413

    STRING: 10090.ENSMUSP00000075609

    UniGene: Mm.273333