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  4. Recombinant Mouse Intraflagellar transport protein 20 homolog (Ift20)

Recombinant Mouse Intraflagellar transport protein 20 homolog (Ift20)

  • 中文名称：
    小鼠Ift20重组蛋白
  • 货号：
    CSB-YP730716MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ift20重组蛋白
  • 货号：
    CSB-EP730716MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ift20重组蛋白
  • 货号：
    CSB-EP730716MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ift20重组蛋白
  • 货号：
    CSB-BP730716MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ift20重组蛋白
  • 货号：
    CSB-MP730716MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ift20
  • Uniprot No.：
  • 别名：
    Ift20; Intraflagellar transport protein 20 homolog; mIFT20
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-132
  • 氨基酸序列
    MAKDILGEAG LHFDELNKLR VLDPEVTQQT VELKEECKDF VDKIGQFQKI VGGLIELVDQ LAKEAENEKM KAIGARNLLK SIAKQREAQQ QQLQALIAEK KTQLERYRVE YEALCKVEAE QNEFIDQFIF QK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Regulates the ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway. Required for protein stability of E3 ubiquitin ligases CBL and CBLB that mediate ubiquitination and internalization of PDGFRA for proper feedback inhibition of PDGFRA signaling. Essential for male fertility. Plays an important role in spermatogenesis, particularly spermiogenesis, when germ cells form flagella. May play a role in the transport of flagellar proteins ODF2 and SPAG16 to build sperm flagella and in the removal of redundant sperm cytoplasm. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.
  • 基因功能参考文献：
    1. intraflagellar transport protein 20 (IFT20) interacts with E3 ubiquitin ligases c-Cbl and Cbl-b and is required for Cbl-mediated ubiquitination and internalization of PDGFRalpha for feedback inhibition of receptor signaling. PMID: 29237719
    2. IFT20 is required for the delivery of the intracellular pool of LAT to the immune synapse in naive primary T lymphocytes. PMID: 26715756
    3. Ciliary proteins Bbs8 and Ift20 promote planar cell polarity in the cochlea. PMID: 25605782
    4. This work is a strong support of a model of opsin transport where IFT20 is involved in the movement from the Golgi complex to the base of the cilium PMID: 24619649
    5. Mxi1 regulates Ift20 promoter activity via Ets-1 binding to the Ift20 promoter. These results indicate that inactivating Mxi1 induces ciliary defects in polycystic kidney. PMID: 23316056
    6. Data show that loss of IFT20 during cone development leads to opsin accumulation in the inner segment even when the connecting cilium and outer segment are still intact. PMID: 21307337
    7. An interaction between SPEF2 and the intraflagellar transport protein IFT20 in the testis. PMID: 19889948
    8. This work suggests that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. PMID: 16775004
    9. IFT20 functions to couple extracellular events to cell proliferation and differentiation and deletion results in cystic kidney diseease. PMID: 18981227
    10. GMAP210 and IFT20 function together at the Golgi in the sorting of proteins destined for the ciliary membrane. PMID: 19112494
    11. IFT20 is the only complex B protein that localizes to the Golgi apparatus. PMID: 19253336

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  • 亚细胞定位：
    Golgi apparatus, cis-Golgi network. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, cilium basal body. Cell projection, cilium. Cytoplasm, cytoskeleton. Golgi apparatus.
  • 组织特异性：
    Expressed predominantly in the testis (at protein level). Expressed in kidney and retina. Expression is up-regulated during spermiogenesis.
  • 数据库链接：

    KEGG: mmu:55978

    STRING: 10090.ENSMUSP00000103910

    UniGene: Mm.358671