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  4. Recombinant Mouse LIM domain and actin-binding protein 1 (Lima1)

Recombinant Mouse LIM domain and actin-binding protein 1 (Lima1)

  • 中文名称：
    小鼠Lima1重组蛋白
  • 货号：
    CSB-YP887660MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Lima1重组蛋白
  • 货号：
    CSB-EP887660MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Lima1重组蛋白
  • 货号：
    CSB-EP887660MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Lima1重组蛋白
  • 货号：
    CSB-BP887660MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Lima1重组蛋白
  • 货号：
    CSB-MP887660MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Lima1
  • Uniprot No.：
  • 别名：
    Lima1; D15Ertd366e; EplinLIM domain and actin-binding protein 1; Epithelial protein lost in neoplasm; mEPLIN
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-753
  • 氨基酸序列
    MESTPFNRRQ WTSLSLRVTA KELSLVNKNK SSAIVEIFSK YQKAAEEANM ERKKNNPESL PQHFRRGTLS VLKKKWENPV AGAEFHTDSL PNSSSEGGHT ADYPPAEVTD KPAPGVRADR EEHTQPKPRF GSRPEAVIQS RYPRSENSHD FKAQATESQK MENCLGDSRH EAEKPETSEN TETSGKIEKY NVPLNRLKMM FEKGEHNQTK SLWTQSRNAG GRRLSENNCS LDDWEIGAGH LSSSAFNSEK NESKRNLELP RLSETSIKDR MAKYQAAVSK QSSPASYTNE LKTSESKTHK WEQKENVPPG PEACSVHQEG SKVSTTENSL VALSVPAEDD TCNSQVKSEA QQPMHPKPLS PDARTSSLPE SSPSKTAKKF QAPAKESCVE CQKTVYPMER LLANQQVFHI SCFRCSYCNN KLSLGTYASL HGRIYCKPHF NQLFKSKGNY DEGFGHKQHK DLWASKSDNE ETLGRPAQPP NAGESPHSPG VEDAPIAKVG VLAASMEAKA SSQREREDKP AETKKLRIAW PPPAELGGSG SALEEGIKVS KPKWPPEDDV CKTEAPEDVD LDLKKLRRSS SLKERSRPFT VAASFRTSSI KSPKASSPSL RKGWSESEQS EEFGGGIATM ERKQTENARP SGEKENVGKS RWQGEEVPRS KDRSSFELES ENFMENGANI AEDDNHVHAQ QSPLEPEAPG WSGFVDTTAA KEFTTQNQKS QDVGFWEGEV VRELSVEEQI KRNRYYDEDE DEE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake.
  • 基因功能参考文献：
    1. this study identifies LIMA1 as a key protein regulating intestinal cholesterol absorption. PMID: 29880681
    2. findings suggest that PINCH-1 regulates integrin-mediated adhesion of keratinocytes through the interactions with ILK as well as EPLIN. PMID: 25609703
    3. Eplin-alpha transcription is regulated by actin-MAL-SRF signalling. PMID: 20236507
    4. EPLIN may function in growth control by associating with and regulating the actin cytoskeleton PMID: 11950948
    5. ERK-mediated phosphorylation of EPLIN contributes to actin filament reorganization and enhanced cell motility. PMID: 17875928
    6. EPLIN functions to link the cadherin-catenin complex to F-actin and simultaneously stabilizes this population of actin fibers, resulting in the establishment of the adhesion PMID: 18093941

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell membrane. Cell projection, ruffle.
  • 组织特异性：
    Highly expressed in the small intestine, including the duodenum, jejunum, and ileum. Low expression in the liver and very low expressed in the heart, spleen, lung, brain, and pancreas. Isoform Alpha is highly expressed in embryos from day 7-11 and in adul
  • 数据库链接：

    KEGG: mmu:65970

    STRING: 10090.ENSMUSP00000073371

    UniGene: Mm.33207