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  4. Recombinant Mouse Lysophosphatidylcholine acyltransferase 1 (Lpcat1), partial

Recombinant Mouse Lysophosphatidylcholine acyltransferase 1 (Lpcat1), partial

  • 中文名称：
    小鼠Lpcat1重组蛋白
  • 货号：
    CSB-YP013052MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Lpcat1重组蛋白
  • 货号：
    CSB-EP013052MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Lpcat1重组蛋白
  • 货号：
    CSB-EP013052MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Lpcat1重组蛋白
  • 货号：
    CSB-BP013052MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Lpcat1重组蛋白
  • 货号：
    CSB-MP013052MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Lpcat1
  • Uniprot No.：
  • 别名：
    Lpcat1; Aytl2; Lysophosphatidylcholine acyltransferase 1; LPC acyltransferase 1; LPCAT-1; LysoPC acyltransferase 1; mLPCAT1; EC 2.3.1.23; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; EC 2.3.1.67; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; Acyltransferase-like 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exhibits both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology. Involved in the regulation of lipid droplet number and size.
  • 基因功能参考文献：
    1. These results provide insight into understanding of the retinal degeneration seen in rd11 mice that lack LPCAT1. PMID: 26887439
    2. LPCAT1 interacts with StarD10 and that amino acids 79-271 of LPCAT1 and the steroidogenic acute regulatory protein-related lipid transfer (START) domain of START domain-containing protein 10 (StarD10) are sufficient for this interaction. PMID: 26048993
    3. Almost wild-type LPCAT1 expression in photoreceptors with strong rod rhodopsin and M/S cone opsin staining, and normal visually-guided water maze behavioral performances were observed in treated rd11 mice. PMID: 24557352
    4. Residue Cys-211 of the conserved motif III is not essential for catalysis and not sufficient for sensitivity to treatment by sulfhydryl-modifier agents. PMID: 22676268
    5. evidence of a protein substrate for Lpcat1 and reveal that histone lipidation may occur through its O-palmitoylation as a novel post-translational modification. PMID: 21685381
    6. LPS appears to destabilize the LPCAT1 protein by GSK-3beta-mediated phosphorylation within a canonical phosphodegron for beta-TrCP docking and site-specific ubiquitination. PMID: 21068446
    7. Studies add another dimension to an essential role for LPCAT1 in retinal photoreceptor homeostasis. PMID: 20713727
    8. full LPCAT1 activity is required to achieve the levels of SatPC essential for the transition to air breathing PMID: 20407208
    9. acyl-CoA:lysophosphatidylcholine acyltransferase 1 (LPCAT1) is predominantly expressed in the lung, in particular in alveolar type II cells. PMID: 16704971
    10. Results suggest that lysophosphatidylcholine acyltransferase (LPCAT) plays a critical role in regulating surfactant phospholipid biosynthesis. PMID: 16864775
    11. Aytl2 gene is the phosphatidylcholine reacylating enzyme in RBCs; this represents the identification of a plasma membrane lysophospholipid acyltransferase and establishes the function of a LPCAT protein PMID: 18156367
    12. LPCAT1 transfers acetate from acetyl-CoA to lyso-PAF; its activity and expression are independent of inflammatory signals PMID: 18285344
    13. Platelet activating factor (PAF) accumulation in spinal cord during experimental allergic encephalomyelitis depends upon up-regulation of activities of both group IV(A) cytosolic phospholipase A2 and acetyl-CoA:lyso-PAF acetyltransferase. PMID: 18802104
    14. LPCAT1 plays an important role in the inactivation of PAF by catalyzing the synthesis of alkyl-PC, an inactivated form of PAF with the use of acyl-CoA and lyso-PAF as substrates. PMID: 19773578

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Lipid droplet.
  • 蛋白家族：
    1-acyl-sn-glycerol-3-phosphate acyltransferase family
  • 组织特异性：
    Predominantly expressed in lung where it is enriched in alveolar type II cells. Expressed at lower levels in spleen and brain. Also detected in erythroleukemic cells and reticulocytes. Weakly or not expressed in other tissues.
  • 数据库链接：

    KEGG: mmu:210992

    STRING: 10090.ENSMUSP00000022099

    UniGene: Mm.284649