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  4. Recombinant Mouse NAD-dependent protein deacetylase sirtuin-4 (Sirt4)

Recombinant Mouse NAD-dependent protein deacetylase sirtuin-4 (Sirt4)

  • 中文名称：
    小鼠Sirt4重组蛋白
  • 货号：
    CSB-YP819171MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Sirt4重组蛋白
  • 货号：
    CSB-EP819171MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Sirt4重组蛋白
  • 货号：
    CSB-EP819171MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Sirt4重组蛋白
  • 货号：
    CSB-BP819171MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Sirt4重组蛋白
  • 货号：
    CSB-MP819171MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Sirt4
  • Uniprot No.：
  • 别名：
    Sirt4; Sir2l4; NAD-dependent protein lipoamidase sirtuin-4; mitochondrial; EC 2.3.1.-; NAD-dependent ADP-ribosyltransferase sirtuin-4; EC 2.4.2.-; NAD-dependent protein deacetylase sirtuin-4; EC 2.3.1.286; Regulatory protein SIR2 homolog 4; SIR2-like protein 4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-333
  • 氨基酸序列
    SGLFV PPSPPLDPEK IKELQRFISL SKKLLVMTGA GISTESGIPD YRSEKVGLYA RTDRRPIQHI DFVRSAPVRQ RYWARNFVGW PQFSSHQPNP AHWALSNWER LGKLHWLVTQ NVDALHSKAG SQRLTELHGC MHRVLCLNCG EQTARRVLQE RFQALNPSWS AEAQGVAPDG DVFLTEEQVR SFQVPCCDRC GGPLKPDVVF FGDTVNPDKV DFVHRRVKEA DSLLVVGSSL QVYSGYRFIL TAREQKLPIA ILNIGPTRSD DLACLKLDSR CGELLPLIDP RRQHSDVQRL EMNFPLSSAA QDP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Does not seem to deacetylate PC. Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels. Down-regulates insulin secretion.
  • 基因功能参考文献：
    1. Loss of sirtuin 4 leads to elevated glucose- and leucine-stimulated insulin levels and accelerated age-induced insulin resistance in mice from multiple genetic backgrounds. PMID: 28726069
    2. SIRT4 is downregulated in cardiomyocytes after myocardial ischemia-reperfusion (MI-R), ameliorates MI-R injury, preserves mitochondrial function and reduces cardiomyocyte apoptosis after MI-R. PMID: 29777709
    3. Data suggest that high-fat diet (HFD) alters regulation of expression of sirtuins (Sirt4 and Sirt7) and enzymes in NAD biosynthetic pathway (Tdo2 and Nnmt); these alterations are more prominent in liver as compared to white adipose tissue or skeletal muscle; Tdo2 and Nnmt may serve as markers of HFD consumption. (Tdo2 = tryptophan 2,3-dioxygenase; Nnmt = nicotinamide N-methyltransferase) PMID: 27592202
    4. These findings identify a robust enzymatic activity for SIRT4, uncover a mechanism controlling branched-chain amino acid flux, and position SIRT4 as a crucial player maintaining insulin secretion and glucose homeostasis during aging. PMID: 28380376
    5. miR-497 modulates cardiac hypertrophy by targeting Sirt4 and may serve as a potential therapeutic substance in the course. PMID: 27992564
    6. Until now, a mammalian cellular lipoamidase has not been characterized; this study discovered that SIRT4 can function with this enzymatic capacity in the mitochondria, and that PDH is a biological substrate; compared to its catalytic efficiency for deacetylation, SIRT4 exhibits far superior enzymatic activity for lipoyl- and biotinyl-lysine modifications. PMID: 25525879
    7. The results of this study indicated a critical and novel stress response role for SIRT4 in promoting proper glutamate transport capacity and protecting against excitotoxicity PMID: 25196144
    8. regulates ATP levels via ANT2 and a feedback loop involving AMPK PMID: 24296486
    9. these results highlight the tumor-suppressive role of SIRT4 in Myc-induced B cell lymphoma and suggest that SIRT4 may be a potential target against Myc-induced and/or glutamine-dependent cancers. PMID: 24368766
    10. The enhanced fatty acid oxidation observed in SIRT4 knockout hepatocytes requires functional SIRT1, demonstrating a clear cross talk between mitochondrial and nuclear sirtuins. PMID: 24043310
    11. Data uncover SIRT4 as an important component of the DNA damage response pathway that orchestrates a metabolic block in glutamine metabolism, cell cycle arrest, and tumor suppression. PMID: 23562301
    12. SIRT4 represses fatty acid oxidation and stimulates lipogenesis. SIRT4 deacetylates and represses malonyl CoA decarboxylase, regulating malonyl coA levels. PMID: 23746352
    13. Data demonstrate that SIRT4 inhibition increases fat oxidative capacity in liver and mitochondrial function in muscle. PMID: 20685656
    14. controls insulin secretion in pancreatic beta cells; also transfers an ADP-ribosyl group from NAD to histones PMID: 16959562
    15. NAD analogues and 32P-NAD were used to study the ADP-ribosyltransferase activity of several different sirtuins, including yeast Sir2, human SirT1, mouse SirT4, and mouse SirT6. PMID: 19220062
    16. Fluorescence in situ hybridization analysis identified a single genomic locus for murine Sirt4 gene on chromosome 5F and is neighbored by the PLA2G1B and PXN genes. PMID: 19306844

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  • 亚细胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    Sirtuin family, Class II subfamily
  • 组织特异性：
    Detected in kidney, heart, brain, liver and pancreatic islets (at protein level).
  • 数据库链接：

    KEGG: mmu:75387

    STRING: 10090.ENSMUSP00000107697

    UniGene: Mm.332616