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  4. Recombinant Mouse Nascent polypeptide-associated complex subunit alpha (Naca)

Recombinant Mouse Nascent polypeptide-associated complex subunit alpha (Naca)

  • 中文名称：
    小鼠Naca重组蛋白
  • 货号：
    CSB-YP713897MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Naca重组蛋白
  • 货号：
    CSB-EP713897MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Naca重组蛋白
  • 货号：
    CSB-EP713897MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Naca重组蛋白
  • 货号：
    CSB-BP713897MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Naca重组蛋白
  • 货号：
    CSB-MP713897MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Naca
  • Uniprot No.：
  • 别名：
    Naca; Nascent polypeptide-associated complex subunit alpha; Alpha-NAC; Alpha-NAC/1.9.2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-215
  • 氨基酸序列
    MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQTATQQ AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVTG VTRVTIRKSK NILFVITKPD VYKSPASDTY IVFGEAKIED LSQQAQLAAA EKFKVQGEAV SNIQENTQTP TVQEESEEEE VDETGVEVKD IELVMSQANV SRAKAVRALK NNSNDIVNAI MELTM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). Isoform 1 and isoform 2 appear to bind DNA and play roles in transcription. Isoform 1 may function as a specific coactivator for JUN, acting to stabilize the interaction of JUN homodimers with promoter elements.
  • 基因功能参考文献：
    1. the skNAC-Smyd1 complex is involved in transcriptional regulation both via the control of histone methylation and histone (de)acetylation. PMID: 26162853
    2. skNAC binds to the E3 SUMO ligase mammalian Mms21/Nse2 and that knockdown of Nse2 expression inhibits specific aspects of myogenic differentiation, accompanied by a partial blockade of the nuclear-to-cytoplasmic translocation of the skNAC-Smyd1 complex PMID: 25002400
    3. depletion of alphaNAC in multiple types of cancer cells induce typical apoptotic cell death. This anti-apoptotic function is mediated by the FADD/c-Jun N-terminal kinase pathway. PMID: 24901053
    4. We propose that maximal transcriptional repression by FIAT requires its interaction with SUMOylated, HDAC2-interacting aNAC PMID: 24375853
    5. Altered gene dosages for Galphas and alpha- NAC in compound heterozygous mice result in reduced bone mass, increased numbers of osteocytes, and enhanced expression of Sost. PMID: 24550008
    6. The bone phenotype of compound Naca(+/-); Fiat(+/-) heterozygotes confirms that FIAT and alphaNAC are part of a common genetic pathway and support a role for the FIAT/alphaNAC interaction in normal bone physiology. PMID: 24440290
    7. data suggest that skNAC controls myoblast migration and sarcomere architecture in a calpain-dependent manner PMID: 23662692
    8. alphaNAC plays a role in regulating gene transcription during mesenchymal cell differentiation by differentially recruiting corepressors at target promoters. PMID: 23092676
    9. nascent polypeptide associated complex regulates specific aspects of myogenesis. PMID: 22154550
    10. The muscle-specific transcription factor skNAC is the major binding partner for Smyd1 in the developing heart. PMID: 21071677
    11. Inhibition of alpha NAC nuclear translocation results in an osteopenic phenotype caused by reduced expression of osteocalcin and type I collagen, accelerated mineralization, and immature woven-bone formation. PMID: 19884350
    12. Similar kinetics of induction and localization of m-Bop and skNAC during the induction of myogenesis in cultured C2C12 cells suggests a possible associated role for these proteins during this process. PMID: 12011100
    13. The osteocalcin gene is a target for the alphaNAC coactivating function and is specifically targeted to the osteocalcin promoter through its DNA-binding activity as a means to achieve increased specificity in gene transcription. PMID: 15831452

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    NAC-alpha family
  • 组织特异性：
    Isoform 1 appears to be ubiquitously expressed.
  • 数据库链接：

    UniGene: Mm.3746