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  4. Recombinant Mouse Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (Pip5k1c)

Recombinant Mouse Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (Pip5k1c)

  • 中文名称：
    小鼠Pip5k1c重组蛋白
  • 货号：
    CSB-YP018027MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pip5k1c重组蛋白
  • 货号：
    CSB-EP018027MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pip5k1c重组蛋白
  • 货号：
    CSB-EP018027MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pip5k1c重组蛋白
  • 货号：
    CSB-BP018027MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pip5k1c重组蛋白
  • 货号：
    CSB-MP018027MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pip5k1c; Kiaa0589; Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma; PIP5K1-gamma; PtdIns(4)P-5-kinase 1 gamma; EC 2.7.1.68; Phosphatidylinositol 4-phosphate 5-kinase type I gamma; PIP5KIgamma
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-661
  • 氨基酸序列
    MELEVPDEAE SAEAGAVTAE AAWSAESGAA AGMTQKKAGL AEAPLVTGQP GPGHGKKLGH RGVDASGETT YKKTTSSTLK GAIQLGIGYT VGNLSSKPER DVLMQDFYVV ESIFFPSEGS NLTPAHHFQD FRFKTYAPVA FRYFRELFGI RPDDYLYSLC NEPLIELSNP GASGSVFYVT SDDEFIIKTV MHKEAEFLQK LLPGYYMNLN QNPRTLLPKF YGLYCVQSGG KNIRVVVMNN VLPRVVKMHL KFDLKGSTYK RRASKKEKEK SLPTYKDLDF MQDMPEGLLL DSDTFGALVK TLQRDCLVLE SFKIMDYSLL LGVHNIDQQE RERQAEGAQS KADEKRPVAQ KALYSTAMES IQGGAARGEA IETDDTMGGI PAVNGRGERL LLHIGIIDIL QSYRFIKKLE HTWKALVHDG DTVSVHRPSF YAERFFKFMS STVFRKSSSL KSSPSKKGRG ALLAVKPLGP TAAFSASQIP SEREDVQYDL RGARSYPTLE DEGRPDLLPC TPPSFEEATT ASIATTLSST SLSIPERSPS DTSEQPRYRR RTQSSGQDGR PQEEPHAEDL QKITVQVEPV CGVGVVPKEE GAGVEVPPCG ASAAASVEID AASQASEPAS QASDEEDAPS TDIYFPTDER SWVYSPLHYS ARPASDGESD T
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility. PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3). PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis. Together with PIP5K1A, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1/cadherin trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor-stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth.
  • 基因功能参考文献：
    1. PIP5K1C regulates nociceptive sensitization in more regions of the nervous system than dorsal root ganglia alone PMID: 29020859
    2. PIPKIgamma and INPP5E localize to the centrosome and coordinate the initiation of ciliogenesis. PMID: 26916822
    3. PIPKIgamma, downstream of EGF and/or HGF receptor, participates in breast cancer progression PMID: 25486426
    4. Shp1 binds and controls PIPKIgamma activity and, thereby, modulates phosphatidylinositol (4,5)-bisphosphate levels and adhesion. PMID: 26101325
    5. The results of this study suggest that PIP5KIgamma, especially PIP5KIgamma_i2, is localized at the periactive zone, a functionally suitable compartment for the endocytosis of synaptic vesicles in photoreceptor ribbon synapses. PMID: 25152467
    6. The lipid kinase PIP5K1C regulates pain signaling and sensitization in mice. PMID: 24853942
    7. The findings suggest an essential role of PIP5KIgamma, particularly PIP5KIgamma_i2, in neuronal migration, possibly through recruitment of adhesion components to the plasma membrane. PMID: 23802628
    8. Although PIP5KIgamma is essential for normal platelet function, individual isoforms of PIP5KIgamma fulfill unique roles for the integrin-dependent integrity of the membrane cytoskeleton and for the stabilization of platelet adhesion. PMID: 23372168
    9. Adult PIPKIgamma(+/-) mice have dramatically elevated hearing thresholds for high-frequency sounds. PMID: 22891314
    10. NMDA receptor activation controls AMPA AMPA AMPA receptor endocytosis during hippocampal LTD by regulating PIP5Kgamma661 activity at postsynapses. PMID: 22243752
    11. PIP5KIgamma-mediated generation of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) at sites of N-cadherin contacts regulates intercellular adhesion strength, an effect due in part to PI(4,5)P2-mediated regulation of gelsolin. PMID: 17538019
    12. these results demonstrate that PIPKIgamma90 is a negative regulator of Ag-induced T cell adhesion and activation PMID: 20855869
    13. PIP5K1C-90 polarization was required for polarized RhoA activation at uropods and provided an initial directional cue for neutrophil polarization on the endothelium. PMID: 20850356
    14. Type I PIPkinases interact with and are regulated by the retinoblastoma susceptibility gene product-pRB. PMID: 11937028
    15. we show that the predominant brain splice variant of PtdInsPKI gamma (PtdInsPKI gamma-90) binds, by means of a short carboxy-terminal peptide, to the FERM domain of talin, and is strongly activated by this interaction PMID: 12422219
    16. we show that the type I phosphatidylinositol phosphate kinase isoform-gamma 661 (PIPKI gamma 661), an enzyme that makes PtdIns(4,5)P(2), is targeted to focal adhesions by an association with talin PMID: 12422220
    17. findings indicate that synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) by the three phosphatidylinositol 4-phosphate 5-kinase (PIP5K) isoforms Ialpha, Ibeta and Igamma is controlled by Rho GTPases PMID: 14681219
    18. Identification of a splice variant of phosphatidylinositol 4-phosphate 5-kinase gamma that is expressed in neurons of the brain. PMID: 14741049
    19. structural model for PIPKIgamma binding to talin at focal adhesions PMID: 15623515
    20. Results suggest that two ARF6 downstream molecules, PIP5K and ACAP1, function together in endosomal tubulation and that phosphoinositide levels may regulate endosomal dynamics. PMID: 17010122
    21. PIP5Kgamma661 is activated by AP-2 complex and generates PI(4,5)P2 in hippocampal neuron synaptic vesicle endocytosis. PMID: 17290217
    22. Type I phosphatidylinositol-4-phosphate 5-kinase (PIP5KI) isoforms have overlapping, but not completely redundant, functions within cells; targeted disruption of PIP5KIgamma causes widespread developmental and cellular defects. PMID: 17609388
    23. CAT-2 exerts control over airway smooth muscle force development through a spermine-dependent pathway that directly correlates with the expression level of PIP-5K-gamma in the lung. PMID: 17644755
    24. These findings demonstrate a unique role for PIP5KIgamma in the anchoring of the cell membrane to the cytoskeleton in megakaryocytes, probably through a pathway involving talin. PMID: 18188447
    25. The beta- and gamma-isoforms of type I PIP5K regulate distinct stages of Ca2+ signaling in mast cells. PMID: 19549683

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endomembrane system. Cytoplasm. Cell junction, focal adhesion. Cell junction, adherens junction. Cell projection, ruffle membrane. Cell projection, phagocytic cup. Cell projection, uropodium. Note=During directional migration isoform 1 localized at the uropodium, and isoform 3 localized all along cell membrane including the uropodium and the leading edge.
  • 组织特异性：
    High expression in brain. Also detected in lung, thymus, heart, testicle, kidney and embryo. Highly expressed in forebrain, in particular in cerebellum, hippocampus and cerebral cortex.
  • 数据库链接：

    KEGG: mmu:18717

    STRING: 10090.ENSMUSP00000100964

    UniGene: Mm.24222