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  4. Recombinant Mouse RAF proto-oncogene serine/threonine-protein kinase (Raf1)

Recombinant Mouse RAF proto-oncogene serine/threonine-protein kinase (Raf1)

  • 中文名称：
    小鼠Raf1重组蛋白
  • 货号：
    CSB-YP859572MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Raf1重组蛋白
  • 货号：
    CSB-EP859572MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Raf1重组蛋白
  • 货号：
    CSB-EP859572MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Raf1重组蛋白
  • 货号：
    CSB-BP859572MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Raf1重组蛋白
  • 货号：
    CSB-MP859572MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Raf1; Craf; RAF proto-oncogene serine/threonine-protein kinase; EC 2.7.11.1; Proto-oncogene c-RAF; cRaf; Raf-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-648
  • 氨基酸序列
    MEHIQGAWKT ISNGFGLKDA VFDGSSCISP TIVQQFGYQR RASDDGKLTD SSKTSNTIRV FLPNKQRTVV NVRNGMSLHD CLMKALKVRG LQPECCAVFR LLQEHKGKKA RLDWNTDAAS LIGEELQVDF LDHVPLTTHN FARKTFLKLA FCDICQKFLL NGFRCQTCGY KFHEHCSTKV PTMCVDWSNI RQLLLFPNST VGDSGVPAPP SFPMRRMRES VSRMPASSQH RYSTPHAFTF NTSSPSSEGS LSQRQRSTST PNVHMVSTTL HVDSRMIEDA IRSHSESASP SALSSSPNNL SPTGWSQPKT PVPAQRERAP GSGTQEKNKI RPRGQRDSSY YWEIEASEVM LSTRIGSGSF GTVYKGKWHG DVAVKILKVV DPTPEQLQAF RNEVAVLRKT RHVNILLFMG YMTKDNLAIV TQWCEGSSLY KHLHVQETKF QMFQLIDIAR QTAQGMDYLH AKNIIHRDMK SNNIFLHEGL TVKIGDFGLA TVKSRWSGSQ QVEQPTGSVL WMAPEVIRMQ DDNPFSFQSD VYSYGIVLYE LMAGELPYAH INNRDQIIFM VGRGYASPDL SRLYKNCPKA MKRLVADCVK KVKEERPLFP QILSSIELLQ HSLPKINRSA SEPSLHRAAH TEDINACTLT TSPRLPVF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. Regulates Rho signaling and migration, and is required for normal wound healing.
  • 基因功能参考文献：
    1. that ablation of c-RAF expression in advanced tumors driven by Kras(G12V)/Trp53 mutations leads to significant tumor regression with no detectable appearance of resistance mechanisms PMID: 29395869
    2. Our findings indicated that miR-195 inhibited WT and L613V RAF-1 induced hyperactive osteoblast differentiation in MC3T3-E1 cells by targeting RAF-1 PMID: 29197556
    3. Data indicate that Raf-1 proto-oncogene, serine-threonine kinase (RAF1) is a negative regulator of hepatocarcinogenesis. PMID: 28000790
    4. Results indicate that proto-oncogene c-RAF (CRAF) function affects postmitotic neural cell differentiation and points to a critical role of CRAF-dependent growth factor signaling pathway in the postmitotic development of adult-born neurons. PMID: 29590115
    5. our studies demonstrate that PDE8A inhibition by enzymatic inhibitors or PDE8A-Raf-1 kinase complex disruptors decreases Teff cell adhesion and migration under flow, and represents a novel approach to target T cells in inflammation PMID: 28851628
    6. Neuroprotective (arylthio)cyclopentenone prostaglandins directly bind to c-Raf protein and protect cells from down-regulation of the c-Raf protein itself, resulting in protection against oxidative stress. PMID: 28888848
    7. We confirmed that Raf1(L613V) knock-in confers a NS-like phenotype, including cardiac hypertrophy. Active RSK3 was increased in Raf1(L613V) mice. Constitutive RSK3 gene deletion prevented the Raf1(L613V)-dependent concentric growth in width of the cardiac myocyte and attenuated cardiac hypertrophy in female mice. PMID: 26940993
    8. C-Raf involves in osteoblast survival in response to mechanical stress. PMID: 27240957
    9. Dual inhibition of c-Raf and soluble epoxide hydrolase by t-CUPM prevents mutant KrasG12D-initiated murine pancreatic carcinoma growth. PMID: 26683769
    10. Simultaneous inhibition of sEH and c-RAF prevents chronic pancreatitis and murine pancreatic intraepithelial neoplasia in LSL-KrasG(1)(2)D/Pdx-1-Cre mice. PMID: 26722025
    11. under normal physiological conditions, PTEN suppresses AKT activity to maintain activation of the RAF1/ERK signaling pathway, which in turn maintains normal function of the initial segment and therefore, normal sperm maturation. PMID: 25512490
    12. Raf-MAPK-dependent psoriatic-like epidermal hyperplasia requires Stat3 signaling in keratinocytes PMID: 23870655
    13. Computational modeling revealed that complexes containing H-ras conformers and galectin-1 affect both the number and lifetime of nanoclusters and thus determine the specific Raf effector recruitment. PMID: 24569991
    14. This EPOR/CR3 system may serve as a useful model to evaluate the unknown Raf kinase pathway and the effects of signal transduction inhibitors for Raf as a target PMID: 23820731
    15. Data indicate that caspase-8 activity is lost upon deletion of c-FLIPL, and p43FLIP rescues caspase-8 activity through Raf1, TRAF2, and RIPK1 association, augmenting ERK and NF-kappaB pathways. PMID: 24275659
    16. Endothelial ERK signaling is critical for both arteriogenesis and arterial-venous patterning and that RAF1 Ser(259) phosphorylation plays a critical role in preventing unopposed ERK activation. PMID: 23529931
    17. Raf1(D486N/+) (here D486N/+) female mice exhibited a mild growth defect. Male and female D486N/D486N mice developed concentric cardiac hypertrophy and incompletely penetrant, but severe, growth defects. PMID: 22826437
    18. Data suggest that K-Ras-responsive tissues exhibit activated Ras/Raf/Erk1/2 signaling, rapidly form premalignant tumors, and activate potent antitumor responses that effectively prevent further malignant progression. PMID: 22532587
    19. C-Raf but not B-Raf is required for the proliferative effects of K-Ras(G12D) in primary epithelial cells. PMID: 22043453
    20. Arrestin-2 and -3 association with beta(2)-adrenergic receptor (beta2AR) significantly enhanced ERK2 binding, but showed little effect on arrestin interactions with the upstream kinases c-Raf1 and MEK1. PMID: 22174878
    21. Raf-1 specifically and acutely regulates insulin 2 mRNA via negative action on Foxo1 PMID: 21817126
    22. Raf1, but not BRAF, modulates BCR-ABL-dependent ERK activation and transformation of hematopoietic cells PMID: 21715303
    23. Data show that through HINT1, the MOR facilitates the cross-talk of two NO- and zinc-regulated signal-transduction pathways, PKC/Src and Raf-1/ERK1/2, implicated in the negative control of morphine effects. PMID: 21235400
    24. c-Raf plays a unique role in mediating K-Ras signaling in non-small cell lung carcinoma and makes it a suitable target for therapeutic intervention. PMID: 21514245
    25. enhanced MEK-ERK activity is critical for causing hypertrophic cardiomyopathy and other RAF1-mutant Noonan syndrome phenotype PMID: 21339642
    26. Results define a novel mechanism of survivin regulation by EGF through the Raf-1/MEK/ERK pathway in pancreatic beta-cells, via prolongation of survivin protein half-life and inhibition of the ubiquitin-mediated proteasomal degradation pathway. PMID: 20807437
    27. Protein kinase C epsilon regulates translocator protein Tspo gene expression through a proto-oncogene protein Raf-1-MAP-kinase signal transduction pathway, acting at least in part through c-Jun and STAT3 transcription factors. PMID: 20469933
    28. Activation of the Raf signaling pathway is involved in the epidermal hyperplasia and the neutrophil-dominant cutaneous inflammatory reactions which are characteristics of psoriasis. PMID: 20188517
    29. analysis of molecular pertubations allowing dysplasia to progress further to adenocarcinoma induced by exaggerted c-Raf kinase activity PMID: 19812696
    30. Rok-alpha inhibition by Raf-1 relies on an intermolecular interaction between the Rok-alpha kinase domain and the cysteine-rich Raf-1 regulatory domain (Raf-1reg). PMID: 19948477
    31. Cyclic AMP blocks cell growth through Raf-1-dependent and Raf-1-independent mechanisms. PMID: 11997508
    32. Raf regulates glycolysis in mouse cells PMID: 12123723
    33. Data indicate that protein kinase C alpha and RAF1 are important in the translational regulation of lipoprotein lipase in adipocytes and that the mechanism of regulation is probably through an ERK-independent pathway. PMID: 12149272
    34. The observed alpha-adrenergic effects on lung neutrophil activation appeared to involve primarily the extracellular signal-regulated kinase pathway at the upstream kinase Raf. PMID: 12223526
    35. elucidation of essential function in erythropoiesis PMID: 12438425
    36. Data suggest that hydrogen peroxide regulates the activity of NF-kappaB by MEK1 activation through a MEKK1-dependent but Ras/Raf-independent mechanism. PMID: 12532330
    37. RafS259 mutants genetically separate MEK/ERK activation from the ability of Raf-1 to induce transformation and differentiation. The results further suggest that RafS259 mutants inhibit signaling pathways required to promote these biological processes. PMID: 12612072
    38. upon activation of protein kinase A, Raf-1 is phosphorylated and 14-3-3 binds, blocking Raf-1 recruitment to the plasma membrane and preventing its activation PMID: 12801936
    39. Raf-1 is dispensable for megakaryocytopoiesis, and for thrombopoietin-induced ERK1/2 activation in primary megakaryocyte-lineage cells. PMID: 14576068
    40. After stimulation of G-protein-coupled receptors, RKIP dissociates from its known target, Raf-1, to associate with GRK-2 and block its activity PMID: 14654844
    41. Raf-1 kinase activity is essential for cardiac hypertrophy and cardiomyocyte survival in response to pressure overload. PMID: 15289381
    42. Ras-independent activation of Raf-1 protein kinase by phorbol myristate acetate and H2O2 is enhanced by src tyrosine kinase inhibitor PP2 PMID: 15356004
    43. These results indicate that Raf-1 promotes cardiomyocyte survival through a MEK/ERK-independent mechanism. PMID: 15467832
    44. a novel Jun-Dmp1 pathway directly links oncogenic Ras-Raf signaling and p19(Arf), independent of the classical cyclin D1/Cdk4-Rb-E2F pathway PMID: 15601844
    45. proteomic analysis of Raf-1 signaling complexes was used to show that Raf-1 counteracts apoptosis by suppressing the activation of mammalian sterile 20-like kinase (MST2) PMID: 15618521
    46. identifed six phosphorylation sites that contribute to the downregulation of Raf-1 after mitogen stimulation; phosphorylation of all six sites requires MEK signaling, indicating a negative feedback mechanism PMID: 15664191
    47. RAF1, protein kinase A, and protein kinase Cepsilon have roles in regulates amphotropic murine leukemia virus envelope protein-induced syncytium formation PMID: 15741175
    48. RAf1 regulates rho signaling and cell migration. PMID: 15753127
    49. Pak1-dependent Raf-1 phosphorylation regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association PMID: 15849194
    50. Raf-1 and A-Raf have a combined role in controlling physiological transient ERK activation and in maintenance of cell cycle progression at its usual rate. PMID: 15856007

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Mitochondrion. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
  • 组织特异性：
    Present in all tissues tested: testis, ovary, small intestine, colon, peripheral blood leukocytes, fetal liver, bone marrow, thymus, lymph node and spleen, and the cell lines melanoma G-361, lung carcinoma A-549, colorectal adenocarcinoma SW480, Burkitt's
  • 数据库链接：

    KEGG: mmu:110157

    STRING: 10090.ENSMUSP00000000451

    UniGene: Mm.184163