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  4. Recombinant Mouse Ribonuclease H2 subunit B (Rnaseh2b)

Recombinant Mouse Ribonuclease H2 subunit B (Rnaseh2b)

  • 中文名称：
    小鼠Rnaseh2b重组蛋白
  • 货号：
    CSB-YP800379MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rnaseh2b重组蛋白
  • 货号：
    CSB-EP800379MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rnaseh2b重组蛋白
  • 货号：
    CSB-EP800379MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rnaseh2b重组蛋白
  • 货号：
    CSB-BP800379MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rnaseh2b重组蛋白
  • 货号：
    CSB-MP800379MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rnaseh2b
  • Uniprot No.：
  • 别名：
    Rnaseh2b; Dleu8Ribonuclease H2 subunit B; RNase H2 subunit B; Deleted in lymphocytic leukemia 8 homolog; Ribonuclease HI subunit B
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-308
  • 氨基酸序列
    AGGRDRGDL AARQLVFLLP EHLKDASKKK KKSSLLFVKL ANPHSGEGAT YLIDMCLQQL FEIKVFKEKH HSWFINQSVQ SGGLLHFATP MDPLFLLLHY LLKAGKEGKY QPLDQVVVDD TFPDCTLLLR FPELEKSLRH VTEEKEVNSK KYYKYSSEKT LKWLEKKVNQ TVVALKANNV NVGARVQSSA YFSGGQVSRD KEEDYVRYAH GLISDYIPKE LSDDLSKFLK LPEPPASLTN PPSKKLKLSD EPVEAKEDYT KFNTKDLKTG KKNSKMTAAQ KALAKVDKSG MKSIDAFFGA KNKKTGKI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.
  • 基因功能参考文献：
    1. Ribonuclease H2 mutations induce a cGAS/STING-dependent innate immune response PMID: 26903602
    2. Recent evidence gathered from ribonuclease H2 knock-out mice provides insight into the molecular mechanisms underlying Aicardi-Goutieres syndrome development and a potential role of DNA damage as a trigger of autoimmunity is discussed. [review] PMID: 23744109
    3. Study performed targeted mutagenesis of the mouse Rnaseh2b gene to gain insight into the in vivo role of the mammalian RNase H2 enzyme. Ablation of Rnaseh2b in mice leads to early embryonic lethality due to elevated DNA damage and reduced cellular proliferation during gastrulation. PMID: 22579044
    4. Data show that the interwoven architecture of RNase H2B and RNase H2C that interface with RNase H2A in a complex ideally suited for nucleic acid binding and hydrolysis coupled to protein-protein interaction motifs. PMID: 19923215
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RNase H2 subunit B family
  • 数据库链接：

    KEGG: mmu:67153

    STRING: 10090.ENSMUSP00000022499

    UniGene: Mm.26040