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  4. Recombinant Mouse Serine palmitoyltransferase 1 (Sptlc1), partial

Recombinant Mouse Serine palmitoyltransferase 1 (Sptlc1), partial

  • 中文名称：
    小鼠Sptlc1重组蛋白
  • 货号：
    CSB-YP022639MO1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Sptlc1重组蛋白
  • 货号：
    CSB-EP022639MO1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Sptlc1重组蛋白
  • 货号：
    CSB-EP022639MO1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Sptlc1重组蛋白
  • 货号：
    CSB-BP022639MO1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Sptlc1重组蛋白
  • 货号：
    CSB-MP022639MO1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Sptlc1
  • Uniprot No.：
  • 别名：
    Sptlc1; Lcb1; Serine palmitoyltransferase 1; Long chain base biosynthesis protein 1; LCB 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs. Required for adipocyte cell viability and metabolic homeostasis.
  • 基因功能参考文献：
    1. mice with an adipocyte-specific deletion of Sptlc1 show that de novo sphingolipid biosynthesis is required for adipocyte cell viability and normal metabolic function and that reduced de novo sphingolipid biosynthesis within adipocytes is associated with adipocyte death, adipose tissue remodeling, and metabolic dysfunction PMID: 28100772
    2. Data suggest that overexpression of serine palmitoyltransferase (Sptlc1, serine palmitoyltransferase long chain base subunit 1) to elevate de novo sphingolipid biosynthesis induces autophagy in the liver. PMID: 25332431
    3. Both Sptlc1 and Sptlc2 interactions are necessary for Serine palmitoyl-CoA transferase (SPT) activity in vivo and SPT activity directly influences plasma sphingolipid levels PMID: 16216550
    4. The expression and activities of SPT in mouse liver increased significantly following fumonisin B1 treatment PMID: 16960033
    5. Physical interaction of ABCA1 and SPTLC1 results in reduction of ABCA1 activity and that inhibition of this interaction produces enhanced cholesterol efflux. PMID: 18484747
    6. Sptlc1 deficient mice absorb less cholesterol. PMID: 19416652

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    Class-II pyridoxal-phosphate-dependent aminotransferase family
  • 组织特异性：
    Expressed in a variety of tissues. Highest expression in brain, kidney and liver. Expressed in brown and white adipose tissues.
  • 数据库链接：

    KEGG: mmu:268656

    STRING: 10090.ENSMUSP00000021920

    UniGene: Mm.240336