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Recombinant Mouse Sodium-dependent dopamine transporter (Slc6a3), partial

  • 中文名称:
    小鼠Slc6a3重组蛋白
  • 货号:
    CSB-YP736814MO
  • 规格:
  • 来源:
    Yeast
  • 其他:
  • 中文名称:
    小鼠Slc6a3重组蛋白
  • 货号:
    CSB-EP736814MO
  • 规格:
  • 来源:
    E.coli
  • 其他:
  • 中文名称:
    小鼠Slc6a3重组蛋白
  • 货号:
    CSB-EP736814MO-B
  • 规格:
  • 来源:
    E.coli
  • 共轭:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名称:
    小鼠Slc6a3重组蛋白
  • 货号:
    CSB-BP736814MO
  • 规格:
  • 来源:
    Baculovirus
  • 其他:
  • 中文名称:
    小鼠Slc6a3重组蛋白
  • 货号:
    CSB-MP736814MO
  • 规格:
  • 来源:
    Mammalian cell
  • 其他:

产品详情

  • 纯度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 别名:
    Slc6a3; Dat; Dat1Sodium-dependent dopamine transporter; DA transporter; DAT; Solute carrier family 6 member 3
  • 种属:
    Mus musculus (Mouse)
  • 蛋白长度:
    Partial
  • 蛋白标签:
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling.
  • 基因功能参考文献:
    1. PREP regulates the function of dopamine transporter, possibly by controlling the phosphorylation and transport of dopamine transporter into the striatum or synaptic membrane. PMID: 27966077
    2. Melatonin MT1 and MT2 receptors interact with dopamine transporter (DAT) in striatum. PMID: 30043140
    3. Study generated a transgenic rat model that overexpresses the mouse DAT gene via pronuclear microinjection. These rats specifically exhibited behavioral and pharmaco-therapeutic phenotype of repetitive disorders. Together, findings suggest that the DAT rat model will constitute a valuable tool for studying the pathological role of DAT overexpression on neural systems relevant to relevant to neuropsychiatric disorders. PMID: 27974817
    4. The findings of thuis study indicated that the DAT Val559 variant induces impulsivity behaviors that are dependent upon the reward context, with increased impulsive action observed when mice are required to delay responding for a reward. PMID: 28964912
    5. These results suggest that DAT expression affects TH expression and phosphorylation largely in DA terminal field compartments. PMID: 27124386
    6. These behavioral and molecular phenotypes indicate that a genetic-driven DAT hypofunction alters neurodevelopmental trajectories consistent with ADHD, but not with schizophrenia and bipolar disorders. PMID: 28454982
    7. An exquisite microanatomical regulation of dopamine by the dopamine transporter was identified in striosomes relative to the matrix in the corpus striatum. PMID: 27036891
    8. Data suggest that environment pollutants methylmercury and 1-methyl-4-phenylpyridinium decrease release of dopamine from dopaminergic neurons; this mechanism involves down-regulation of expression of Slc6a3. PMID: 26056851
    9. This study show that Dopamine transporter is enriched in filopodia and induces filopodia formation. PMID: 25936602
    10. The sigma-1R deficiency through suppressing NR2B function and DAT expression can reduce MPTP-induced death of dopaminergic neurons and parkinsonism. PMID: 26203861
    11. DAT gene knockout in mice results dendritic spine loss in pyramidal neurons in the CA1 field of the hippocampus. PMID: 25817859
    12. Results show that moderate increases in DAT function cause spontaneous dopaminergic cell loss, oxidative stress and fine motor impairment that is reversed by l-DOPA treatment PMID: 25447236
    13. Chronic and acute reductions of DAT functioning in mice impaired decision-making. PMID: 25005251
    14. These results demonstrate that the presence of the N-terminal tag leads to impaired DAT protein expression in vivo due in part to improper trafficking of the tagged transporter. PMID: 24886986
    15. studies demonstrate an in vivo functional impact of the DAT Val559 variant, providing support for the ability of DAT dysfunction to impact risk for mental illness. PMID: 25331903
    16. The data of this study imply that individual differences in DAT expression (either genetically or pharmacologically induced) may affect susceptibility to addiction of different types of psychostimulants. PMID: 24673634
    17. findings support the idea that altered DAT and VMAT2 expression affect age-related changes in dopaminergic function. PMID: 23978383
    18. This study demonistrated that environment enhanced slc6a2 expression in brain and enhanced totaroad perfermance and short term working menory. PMID: 23558143
    19. DAT C-terminal protein-protein interactions are critical for AMPH-evoked DA efflux and suggest that it may be possible to target protein-protein interactions to modulate transporter function and interfere with psychostimulant effects. PMID: 23884410
    20. dopamine transporter is a direct target of Lmx1a and emphasizes a novel role of Lmx1a as one of regulators of mature midbrain dopaminergic neurotransmitter phenotypes PMID: 22564125
    21. Increased surface dopamine transporter expression upon dopamine D2 autoreceptor activation resulted from enhanced dopamine transporter recycling in the striatum. PMID: 23458603
    22. PDZ-domain interactions are critical for synaptic distribution of dopamine transporter in vivo and thereby for proper maintenance of dopamine homoeostasis. PMID: 23481388
    23. DAT expression was increased by Nurr1 gene activation in lactacystin-lesioned mice. PMID: 22483304
    24. This study demonistrated that Cortico-subcortical neuromodulation involved in the amelioration of prepulse inhibition deficits in dopamine transporter knockout mice. PMID: 22781838
    25. DAT-deficient mice exhibit hyperlocomotive phenotype along with reduced anxiety behavior. PMID: 22572477
    26. investigation of role of serotonergic and dopaminergic receptors in cognitive defects in model of schizophrenia in knockout mice: Response of cognitive defects to nicotine (e.g., to smoking) appears to involve both Dat1 and 5-HT1A serotonin receptor. PMID: 22809709
    27. in the early pathogenesis of PD, alpha-synuclein acts as a fine modulator of the dopaminergic synapse by regulating the subcellular distribution of key proteins such as the DAT PMID: 22163275
    28. Altogether, these data support an important role for GDNF in the regulation of uptake, synthesis, and metabolism of DA during aging. PMID: 21144620
    29. DAT and Oct3 modulate nigrostriatal damage induced by PQ(2+)/PQ(+) redox cycling PMID: 22143804
    30. alpha-Synuclein stimulates a dopamine transporter-dependent chloride current and modulates the activity of the transporter PMID: 21990355
    31. Demonstrate that DAT knockout mice chose the riskier options more than wild type mice, providing further support for the use of DAT KO mice as a model of biopolar disorder mania. PMID: 21421642
    32. Mice lacking the dopamine transporter have elevated dopaminergic tone and represent a genetic animal model in which certain endophenotypes of ADHD can be recapitulated. PMID: 21432587
    33. Dopamine transporter mRNA levels in the ventral tegmental area did not significantly differ between mice selectively bred for high or low stress reactivity. PMID: 20451634
    34. DAT KO mice appear, at this level of anatomic resolution, to have preserved cortico-striatal-thalamic connectivity but diminished robustness of reward-modulating circuitry distal to the thalamus. PMID: 20634895
    35. DAT is constitutively internalized and sorted in a ubiquitination-independent manner to late endosomes/lysosomes and in part to a Rab4 positive short loop recycling pathway. PMID: 20551317
    36. data are consistent with the view that tonic dopamine affects the sensitivity of an organism to external reward signals, and that this increased motivation for reward of DAT knockdown mice lowers the threshold for initiating responding in a timing task PMID: 20167208
    37. These results strongly suggest that DAT glycosylation is involved in the differential vulnerability of midbrain dopamine cells in Parkinson's disease. PMID: 19766189
    38. Dopamine transporter blockade by cocaine appears a sufficient explanation for cocaine-induced locomotion. Variation in DAT appears to cause differences in locomotion without drug stimulation. PMID: 11803442
    39. 3-NP induced motor impairment in DAT-/- mice. There was significant neuron degeneration in the neostriatum also. This suggests dopamine modulation of excitotoxicity within the nigrostriatal system. PMID: 12099912
    40. Data demonstrate that estrogen exerts significant modulatory effects on olfactory bulb function through modulation of tyrosine hydroxylase, dopamine transporter and norepinephrine transporter expression. PMID: 12480184
    41. Deletions of the dopamine transporter gene increase ethanol consumption in male knockout mice, while female knockout mice had even higher ethanol preferences. PMID: 12655306
    42. DAT knockdown mutant mice have higher food and water intake. In a runway task, they demonstrated enhanced acquisition and greater incentive performance for a sweet reward but minus higher orofacial "liking" reactions PMID: 14561867
    43. ability of cocaine to inhibit DAT is directly related to its reinforcing actions. However, mice with a genetic deletion of the DAT still experience the rewarding effects of cocaine. This suggests that there is an alternate site for cocaine reinforcement. PMID: 14691264
    44. The observed of this study found that the genetic background dramatically affects phenotypes previously reported on DAT knockout (KO) mice. PMID: 15245485
    45. studies suggest that parkin increases dopamine uptake by enhancing the ubiquitination and degradation of misfolded dopamine transporter (DAT) PMID: 15492001
    46. In five extinction sessions in which food is no longer delivered by nose poking, homozygous dopamine transporter-deficient mice exert significantly more responses than wild-type mice. PMID: 15542711
    47. an association between homozygosity for 10-repeat allele at DAT1 and the DAT density assessed in vivo and correlation between the homozygosity for 10-repeat allele PMID: 15572278
    48. Changes in innate and acquired immune responses in mice with targeted deletion of the dopamine transporter gene. PMID: 15748955
    49. Disruption of the Dat1 gene in mice leads to two different phenotypes; one related to anxiety-reducing and novelty seeking, while the other has some homology to disorders with a stereotypical-perseverative spectrum. PMID: 15856082
    50. Results suggest that blockade of the dopamine transporter DAT is necessary for cocaine reward in mice with a functional DAT. PMID: 16754872

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  • 亚细胞定位:
    Cell membrane; Multi-pass membrane protein. Cell projection, neuron projection.
  • 蛋白家族:
    Sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family, SLC6A3 subfamily
  • 组织特异性:
    Found in the substantia nigra and ventral tegmental dopamine neurons, in fibers of the medial forebrain bundle ascending into the striatum, and within dense fiber networks and varicosities in the dorsal and ventral striatum (at protein level). Lower expre
  • 数据库链接:

    KEGG: mmu:13162

    STRING: 10090.ENSMUSP00000022100

    UniGene: Mm.41993