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  4. Recombinant Mouse Sperm-associated antigen 16 protein (Spag16)

Recombinant Mouse Sperm-associated antigen 16 protein (Spag16)

  • 中文名称：
    小鼠Spag16重组蛋白
  • 货号：
    CSB-YP815676MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Spag16重组蛋白
  • 货号：
    CSB-EP815676MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Spag16重组蛋白
  • 货号：
    CSB-EP815676MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Spag16重组蛋白
  • 货号：
    CSB-BP815676MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Spag16重组蛋白
  • 货号：
    CSB-MP815676MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Spag16
  • Uniprot No.：
  • 别名：
    Spag16; Pf20; Sperm-associated antigen 16 protein; Pf20 protein homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-639
  • 氨基酸序列
    MAAPSGVPPL RVLEELGIGL SPTGEVTEAV TSEGAYYLEQ VTITETSEDE CEYEEIPDDN FSIPEGEEDL EKAIHIIGEQ ARDIHILEQQ TILPARNVMQ EAIEDFLCNF LIKMGMTRTL DCFQAEWYEL IQKKGSDFKG LGNVPDVYSQ VMLLETENKN LKKELKHFKQ AAEKAKEDLL RTQKERDFHR MHHKRIVQEK NKLIADLKGL KLHYASYEPT IRVLHEKHHA LLKEKMLTSL ERDRAVGKIS GLQATLKNID MGHIQVPVIK GSYESASITR ESGDRAGHSC EKENSSEGPT QKSLREAREE VGYKSKLKNE KKDSEFPVDM QPDPNVTSCT ENVSAAKFDY KLNNIFRLHE LPVSCIVMHP CRDYLISCSE DRLWKMVGLP QGNVLLTGSG HTDWLSGCCF HPSGSKLATS SGDSTIKLWD LNKGECTLTL EGHNHAVWSC TWHSCGDFVA SASLDMTSKI WDVNSERCRY TLYGHTDSVN SIEFFPFSNI LLTASADKTL SVWDARTGKC EQSLYGHMHS VNDATFTPRG HIIASCDARG VTKLWDFRKL IPIVSIDVGP SSGNEVNFDQ SGRVLAQASA NGIIHLLDLK SGQIHKLVGH ESEVHSVVFS HLGENLYSGG SDGTIRLWI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia.
  • 基因功能参考文献：
    1. The distinct nuclear localization of SPAG16S and its ability to modulate Spag16L mRNA expression suggest that SPAG16S plays an important role in the gene expression machinery of male germ cells. PMID: 21655194
    2. Functional deficiencies and a reduced response to calcium in the flagellum of mouse sperm lacking SPAG16L. PMID: 20042536
    3. In sperm, SPAG16L is found in the central apparatus of the axoneme. We conclude that SPAG16L is essential for sperm flagellar function. PMID: 16382026
  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm, cytoskeleton, flagellum axoneme. Cell projection, cilium, flagellum.
  • 组织特异性：
    Expressed in testis.
  • 数据库链接：

    KEGG: mmu:66722

    STRING: 10090.ENSMUSP00000069821

    UniGene: Mm.440554