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  4. Recombinant Mouse T-box transcription factor TBX5 (Tbx5)

Recombinant Mouse T-box transcription factor TBX5 (Tbx5)

  • 中文名称：
    小鼠Tbx5重组蛋白
  • 货号：
    CSB-YP023257MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Tbx5重组蛋白
  • 货号：
    CSB-EP023257MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Tbx5重组蛋白
  • 货号：
    CSB-EP023257MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Tbx5重组蛋白
  • 货号：
    CSB-BP023257MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Tbx5重组蛋白
  • 货号：
    CSB-MP023257MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tbx5; T-box transcription factor TBX5; T-box protein 5
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-518
  • 氨基酸序列
    MADTDEGFGLARTPLEPDSKDRSCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHE RELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNK WSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQ PRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSD DLELHRMSRMQSKEYPVVPRSTVRHKVTSNHSPFSSETRALSTSSNLGSQYQCENGVSGP SQDLLPPPNPYPLAQEHSQIYHCTKRKDEECSSTEHPYKKPYMETSPSEEDTFYRSGYPQ QQGLSTSYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDR LPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPG GLQPPEFLYTHGVPRTLSPHQYHSVHGVGMVPEWSENS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation. Binds to the core DNA motif of NPPA promoter.
  • 基因功能参考文献：
    1. Enrichment of induced cardiomyocytes derived from mouse fibroblasts can be achieved by reprogramming with cardiac transcription factors, Gata4, MEF2c, Tbx5, and Hand2. PMID: 29257325
    2. Defined a transcriptional architecture for atrial rhythm control organized as an incoherent feed-forward loop, driven by TBX5 and modulated by PITX2. TBX5/PITX2 interplay provides tight control of atrial rhythm effector gene expression, and perturbation of the co-regulated network caused atrial fibrillation susceptibility. PMID: 27582060
    3. The data also suggest that, in human, KLF13 may be a genetic modifier of the Holt-Oram Syndrome gene TBX5. PMID: 28164238
    4. Our data demonstrate an early, inherent asymmetry in the left and right limb-forming regions and that threshold levels of Tbx5 are required to overcome this asymmetry to ensure symmetric forelimb formation. PMID: 27992425
    5. Data show that three transcriptional factors Gata4, Mef2c, and Tbx5 (abbreviated as GMT) significantly improved murine embryonic stem cells (ESCs) differentiated into cardiomyocytes. PMID: 26449528
    6. defines a TBX5-nucleosome remodeling and deacetylase interaction essential to cardiac development and the evolution of the mammalian heart PMID: 26859351
    7. Study reports extensive and complex interdependent genomic occupancy of TBX5, NKX2-5, and the zinc finger TF GATA4 coordinately controlling cardiac gene expression, differentiation, and morphogenesis. PMID: 26875865
    8. Tbx5 and Osr1 interact to regulate posterior second heart field cell cycle progression for cardiac septation. PMID: 25986147
    9. Haploinsufficiency of Tbx5 and trisomy affects alignment of the aorta and this effect may stem from deviations from normal left-right patterning in the heart; study unveiled a previously unknown interaction between the Tbx5 gene and trisomy, suggesting a connection between Tbx5 and trisomic genes important during heart development. PMID: 26208718
    10. These findings elucidate mechanisms regulating the commitment of mesodermal cells in the early embryo and identify the Tbx5 cardiac transcriptome. PMID: 24677751
    11. our findings reveal a novel mechanism for regulation of SCFFbox25-dependent Nkx2-5 and Tbx5 ubiquitination in cardiac development and provide a new insight into the regulatory mechanism of Nkx2-5 and Tbx5 transcriptional activity. PMID: 25725482
    12. Our findings implicate Foxf genes in atrioventricular septation, describe the molecular underpinnings of the genetic interaction between Hedgehog signaling and Tbx5, and establish a molecular model for the selection of the SHF gene regulatory network PMID: 25356765
    13. Disruption of myocardial Gata4 and Tbx5 results in defects in cardiomyocyte proliferation and atrioventricular septation. PMID: 24858909
    14. TBX5 isoforms derived from novel exons have distinct expression domains and function. Alternative splicing regulates TBX5 function in heart and limb. PMID: 25623069
    15. Tbx5 is a common node in the genetic pathways regulating forelimb and sternum development, enabling specific adaptations of thesefeatures without affecting other skeletal elements. PMID: 25468972
    16. Data show that Hdac3 physically interacts with Tbx5 and modulates its acetylation to repress Tbx5-dependent activation of cardiomyocyte lineage-specific genes. PMID: 24565863
    17. SRC-2 is critical to transcriptional control modulated by MEF2, GATA-4, and Tbx5, thereby enhancing gene expression associated with cardiac growth. PMID: 24811170
    18. Data indicate that Nkx2-5, Tbx5, Gata4, or Myocd alone did not induce the de novo expression of cardiac marker proteins in 10T1/2 non-myoblastic cells. PMID: 23144723
    19. miR-218 is a crucial mediator of Tbx5 in heart development PMID: 23226307
    20. The gene transfer of three cardiac transcription factors, Gata4, Mef2c, and Tbx5 (GMT), induced cardiomyocyte-like cells in infarcted hearts in mice. PMID: 22931955
    21. Tbx5 regulated second heart field Gas1 and Osr1 expression, supporting both pathways. PMID: 22898775
    22. Hox genes act upstream of Tbx5 to control the axial position of forelimb formation. PMID: 22872086
    23. a TBX5-responsive enhancer downstream of Scn5a is sufficient to drive ventricular conduction system expression in vivo, dependent on canonical T-box binding sites PMID: 22728936
    24. Induction of Gata4/Mef2c/Tbx5 overexpression in fibroblasts is inefficient at inducing molecular and electrophysiological phenotypes of mature cardiomyocytes. PMID: 22581928
    25. Tbx5 overexpression favors a first heart field lineage in murine embryonic stem cells and in Xenopus laevis embryos. PMID: 22072574
    26. the interaction of myocardin and Tbx5 maybe involved in cardiac development and diseases PMID: 21897873
    27. Nos3, a gene regulated by many congenital heart disease risk factors including stress and diabetes, interacts genetically with Tbx5 provides a molecular framework to understand gene-environment interaction in the setting of human birth defects. PMID: 20974940
    28. Tbx5 in forelimbs or (Tbx4 in hindlimbs) specifically affects muscle and tendon patterning without disrupting skeletal development, thus suggesting that distinct cues regulate these processes. PMID: 20152185
    29. Tbx5 is not essential for the early establishment of the limb field in the lateral plate mesoderm but is a primary and direct initiator of forelimb bud formation. PMID: 12490567
    30. Data indicate that Tbx5 specifies the identity of the left ventricle through tight interactions among several heart-specific factors. PMID: 14573514
    31. data demonstrate clear differences in both the expression and function of Tbx5 and Tbx20 and suggest that the modulation of cardiac gene expression can occur as a result of combinatorial regulatory interactions of T-box proteins PMID: 14978031
    32. Required for Cx40-independent patterning of the cardiac conduction system. PMID: 15289437
    33. Tbx5 is not sufficient to determine limb-specific morphologies but have a roles in initiating limb outgrowth. PMID: 15621531
    34. NKX2.5 inhibits myocyte differentiation and myotube formation, and up-regulates Gata4 and Tbx5 expression PMID: 15653675
    35. left ventricular diastolic function of Tbx5(del/+) mice is significantly deteriorated, whereas the systolic function remains normal PMID: 15849237
    36. Results suggest that T-box transcription factor 5 regulation of connexin 40 plays a critical role in the exquisite developmental patterning of the forelimbs and sternum. PMID: 15923624
    37. A positive and negative feed-forward circuit between Tbx5 and Sall4 ensures precise patterning of embryonic limb and heart and provides a unifying mechanism for heart/hand syndromes. PMID: 16380715
    38. expression of p204 is activated by the cardiac Gata4, Nkx2.5, and Tbx5 proteins PMID: 16556595
    39. there is an exquisite sensitivity of the developing heart to Tbx5 dosage PMID: 16870172
    40. Tbx5 is dispensdable for forelimb outgrowth. PMID: 17138667
    41. Tbx18 interacts with Gata4 and Nkx2-5 and competes Tbx5-mediated activation of the cardiac Natriuretic peptide precursor type a-promoter. Tbx18 down-regulates Tbx6-activated Delta-like 1 expression in the somitic mesoderm in vivo PMID: 17584735
    42. We conclude that a molecular pathway including Tbx5, Nkx2-5, and Id2 coordinates specification of ventricular myocytes into the ventricular conduction system lineage. PMID: 17604724
    43. haploinsufficiency of Tbx5 in mouse results in cellautonomous defects in ventricular relaxation PMID: 18378906
    44. alternatively spliced Tbx5 isoforms have roles in cell growth and differentiation PMID: 18391012
    45. Chromatin immunoprecipitation experiments revealed that Mef2C, Tbx5, and titin are direct target genes of HIF1alpha in vivo. Thus, hypoxia signaling controls cardiac development through HIF1alpha-mediated transcriptional regulation PMID: 18849322
    46. These findings highlight the unique genetic interactions of Gata4 and Gata6 with Tbx5 for normal cardiac morphogenesis in vivo. PMID: 19084512

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 数据库链接：

    KEGG: mmu:21388

    STRING: 10090.ENSMUSP00000018407

    UniGene: Mm.103636