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  4. Recombinant Mouse Three-prime repair exonuclease 1 (Trex1)

Recombinant Mouse Three-prime repair exonuclease 1 (Trex1)

  • 中文名称：
    小鼠Trex1重组蛋白
  • 货号：
    CSB-YP821009MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Trex1重组蛋白
  • 货号：
    CSB-EP821009MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Trex1重组蛋白
  • 货号：
    CSB-BP821009MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Trex1重组蛋白
  • 货号：
    CSB-MP821009MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Trex1
  • Uniprot No.：
  • 别名：
    Trex1; Three-prime repair exonuclease 1; EC 3.1.11.2; 3'-5' exonuclease TREX1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-314
  • 氨基酸序列
    MGSQTLPHGH MQTLIFLDLE ATGLPSSRPE VTELCLLAVH RRALENTSIS QGHPPPVPRP PRVVDKLSLC IAPGKACSPG ASEITGLSKA ELEVQGRQRF DDNLAILLRA FLQRQPQPCC LVAHNGDRYD FPLLQTELAR LSTPSPLDGT FCVDSIAALK ALEQASSPSG NGSRKSYSLG SIYTRLYWQA PTDSHTAEGD VLTLLSICQW KPQALLQWVD EHARPFSTVK PMYGTPATTG TTNLRPHAAT ATTPLATANG SPSNGRSRRP KSPPPEKVPE APSQEGLLAP LSLLTLLTLA IATLYGLFLA SPGQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
  • 基因功能参考文献：
    1. Trex1 is an upstream regulator of radiation-driven anti-tumor immunity. Trex1 induction may guide the selection of radiation dose and fractionation in patients treated with immunotherapy. PMID: 28598415
    2. the cell-cycle-dependent post-translation modification of TREX1 regulates its interaction with OST. PMID: 28297665
    3. The effect of topical TREX1 knockdown and local interferon production on HIV transmission in human cervicovaginal explants and humanized mice, is reported. PMID: 27184854
    4. data do not support the concept of retroelement-derived cDNA as key triggers of systemic autoimmunity in Trex1-deficient humans and mice PMID: 28835460
    5. Trex1 expression in dendritic cells is essential to prevent breakdown of self-tolerance ensuing from aberrant detection of endogenous DNA. PMID: 27511730
    6. Data show that oligosaccharyltransferase (OST) activity is dysregulated in three prime exonuclease 1 knockout (Trex1-/-) cells. PMID: 26320659
    7. Data show that cyclic GMP-AMP synthase (cGAS) is essential for all aspects of the autoimmune disease in 3' repair exonuclease Trex1 knockout mice. PMID: 26223655
    8. Dysfunctional dsDNA degradation by TREX1 D18N induces disease in mice that recapitulates many characteristics of human lupus. PMID: 25848017
    9. knocking out the DNA sensor cyclic GMP-AMP synthase completely abrogates spontaneous induction of IFN-stimulated genes in TREX1-deficient cells. PMID: 24813208
    10. Spontaneous type I INF dependent cutaneous pathology in TREX1 deficiency illustrates common pathogenetic pathway in chilblain lupus. PMID: 24270665
    11. Upon proinflammatory stimulation, Trex1(-/-) macrophages increase CD86, TNF-alpha & IFN-alpha production, & Ag presentation to CD4(+) T cells, but decrease apoptotic T cell clearance. Trex1 is a negative regulator of macrophage inflammatory activation. PMID: 24218451
    12. Oxidized DNA Is less susceptible to TREX1 degradation; the oxidized base 8-hydroxyguanosine, a marker of oxidative damage in DNA, potentiated cytosolic immune recognition by decreasing its susceptibility to 3' repair exonuclease 1 -mediated degradation PMID: 23993650
    13. regulates lysosomal biogenesis and interferon-independent activation of antiviral genes PMID: 23160154
    14. The structures of the mutant TREX1 proteins provide insight into the dysfunction relating to human disease. PMID: 22071149
    15. TREX1 bound to cytosolic HIV DNA and digested excess HIV DNA that would otherwise activate interferon expression via a pathway dependent on the kinase TBK1, the adaptor STING and the transcription factor IRF3 PMID: 20871604
    16. Trex1(-/-) mice exhibit a dramatically reduced survival and develop inflammatory myocarditis leading to progressive, often dilated, cardiomyopathy and circulatory failure PMID: 15254239
    17. Study defines Trex1 as an essential negative regulator of the IFN-stimulatory DNA response and delineate the genetic pathway linking Trex1 deficiency to lethal autoimmunity. PMID: 18724932
    18. Study showed that GFP-TREX1-(1-307) lacking the C-terminal seven amino acids localizes in a perinuclear pattern. PMID: 19442247

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein. Note=Retained in the cytoplasm through the C-terminal region. In response to DNA damage, translocates to the nucleus where it is specifically recruited to replication foci. Translocation to the nucleus also occurs during GZMA-mediated cell death.
  • 蛋白家族：
    Exonuclease superfamily, TREX family
  • 组织特异性：
    Widely expressed with high expression levels detected in spleen, thymus and uterus.
  • 数据库链接：

    KEGG: mmu:22040

    STRING: 10090.ENSMUSP00000050971

    UniGene: Mm.439964