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  4. Recombinant Mouse Transcription factor E2-alpha (Tcf3)

Recombinant Mouse Transcription factor E2-alpha (Tcf3)

  • 中文名称：
    小鼠Tcf3重组蛋白
  • 货号：
    CSB-YP023301MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Tcf3重组蛋白
  • 货号：
    CSB-EP023301MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Tcf3重组蛋白
  • 货号：
    CSB-EP023301MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Tcf3重组蛋白
  • 货号：
    CSB-BP023301MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Tcf3重组蛋白
  • 货号：
    CSB-MP023301MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tcf3; Alf2; Me2; Tcfe2a; Transcription factor E2-alpha; Immunoglobulin enhancer-binding factor E12/E47; Transcription factor 3; TCF-3; Transcription factor A1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-651
  • 氨基酸序列
    MMNQSQRMAP VGSDKELSDL LDFSMMFPLP VANGKSRPAS LGGTQFAGSG LEDRPSSGSW GSSDQNSSSF DPSRTYSEGA HFSDSHSSLP PSTFLGAGLG GKGSERNAYA TFGRDTSVGT LSQAGFLPGE LSLSSPGPLS PSGIKSSSQY YPSFPSNPRR RAADGGLDTQ PKKVRKVPPG LPSSVYPPSS GDSYSRDAAA YPSAKTPSSA YPSPFYVADG SLHPSAELWS TPSQVGFGPM LGDGSSPLPL APGSSSVGSG TFGGLQQQDR MGYQLHGSEV NGSLPAVSSF SAAPGTYSGT SGHTPPVSGA AAESLLGTRG TTASSSGDAL GKALASIYSP DHSSNNFSPS PSTPVGSPQG LPGTSQWPRA GAPSALSPNY DAGLHGLSKM EDRLDEAIHV LRSHAVGTAS DLHGLLPGHG ALTTSFTGPM SLGGRHAGLV GGSHPEEGLT SGASLLHNHA SLPSQPSSLP DLSQRPPDSY SGLGRAGTTA GASEIKREEK EDEEIASVAD AEEDKKDLKV PRTRTSPDED EDDLLPPEQK AEREKERRVA NNARERLRVR DINEAFKELG RMCQLHLSSE KPQTKLLILH QAVAVILSLE QQVRERNLNP KAACLKRREE EKVSGVVGDP QLALSAAHPG LGEAHNPAGH L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Together with TCF15, required for the mesenchymal to epithelial transition. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.
  • 基因功能参考文献：
    1. this study identifies E2A target genes in embryonic neural stem cells and demonstrates that E47 regulates neuronal differentiation via p57(KIP2). PMID: 28939666
    2. If Gfi1 levels fall below a threshold, Id1 expression increases and renders E2A unable to function, which prevents hematopoietic progenitors from engaging along the B lymphoid lineage PMID: 27467586
    3. Data suggest a novel mechanism of drug resistance in which E2a and PRC2 drive changes in the B-cell epigenome; these alterations attenuate alkylating agent treatment-induced apoptosis. PMID: 27146673
    4. These findings suggest that miR-506-3p played an important role in regulating NSC proliferation and differentiation via targeting TCF3, and provide a promising avenue for future in-depth research into the functions of miR-506-3p and TCF3 in nervous system development. PMID: 27538704
    5. Conditional expression of E2A-HLF induces B-cell precursor death and myeloproliferative-like disease in knock-in mice. PMID: 26588248
    6. Upregulation of E12/E47 by HBx ultimately and concomitant repression of E-cadherin expression led to epithelial-mesenchymal transition in human hepatocytes. PMID: 26490105
    7. Mechanistically, E47 repressed the expression of several astrocyte-specific genes in adult NSPCs. PMID: 26438726
    8. Tcf3 is upregulated in skin wounds and Tcf3 overexpression accelerates keratinocyte migration and skin wound healing. PMID: 24909826
    9. Loss and gain of function analyses combined with in vivo studies in syngeneic breast cancer models demonstrate the participation of LOXL2 and E47 in tumor growth and their requirement for lung metastasis. PMID: 24632622
    10. constitutive expression distinguishes tumor-resident monocyte-derived myeloid suppressor cells PMID: 25500368
    11. Tcf3 has a role in repressing Wnt-beta-catenin signaling and maintaining neural stem cell population during neocortical development PMID: 24832538
    12. Transcription of RORgammat in developing Th17 cells is regulated by E-proteins. PMID: 24064669
    13. the role of Tcf3 in cell-fate decision is more complex than previously appreciated and is highly dependent on cellular context PMID: 25038042
    14. Id2 represses E2A-mediated activation of IL-10 expression in T cells. PMID: 24723679
    15. transcription factor E2A binds to the UNG2 promoter and represses UNG2 expression. PMID: 24747958
    16. pre-BCR signaling downregulates several components of the recombination machinery, including RAG1, RAG2, and PAX5, through calmodulin inhibition of E2A. PMID: 24470503
    17. NF-kappaB potentiated the binding of E2A to an E-box motif located immediately downstream of the 2 closely-spaced transcription start sites for sustained 14-3-3gamma expression and CSR induction. PMID: 23851690
    18. Cell-intrinsic in vivo requirement for the E47-p21 pathway in long-term hematopoietic stem cells. PMID: 24259504
    19. Id2 restrains CD8(+) T cell memory differentiation by inhibiting E2A-mediated direct activation of Tcf7 and that Id2 expression. PMID: 23536629
    20. Wnt signaling down-regulates Tcf3 expression, possibly at both the transcriptional and post-transcriptional levels.. it is a novel mechanism through which Wnt signaling inhibits neuro-ectodermal lineage differentiation PMID: 23658527
    21. E2A transcription factors limit expression of Gata3 to facilitate T lymphocyte lineage commitment. PMID: 23297135
    22. Protein inhibitor of activated STAT, PIASy regulates alpha-smooth muscle actin expression by interacting with E12 in mesangial cells. PMID: 22829926
    23. A direct physical interaction between beta-catenin and Tcf3 in the nucleus was confirmed by co-immunoprecipitation studies. and the inhibitory beta-catenin/Tcf3 interface was independent of the ability of Tcf3 to directly interact with DNA. PMID: 22270545
    24. These results thus implicate the E2A-CDKN1A transcriptional axis in the control of megakaryopoiesis and reveal the lineage-selective inhibition of this axis as a likely mechanistic basis for the inhibitory effects of cAMP signaling. PMID: 22514271
    25. the feedback inhibition of the BCR signalosome is to a large extent through inhibition of the transcription factor E2A by Ca(2+)/calmodulin PMID: 22581853
    26. Wnt/beta-catenin counteracts Tcf3 repression of Lef1, which subsequently activates target gene expression via Lef1-beta-catenin complexes. PMID: 22573616
    27. studies show that the entire ensemble of Id proteins has the ability to interact with E47, identify factors that associate with E47, and reveal a spectrum of phosphorylated residues in E47, including an AKT substrate site PMID: 22354994
    28. we revealed the presence of five transcription start sites within 50 bp of the mTcf3 promoter region, providing a 298 bp long 5'-UTR upstream of the mTcf3 translation start site PMID: 21935611
    29. The data suggest that E2A directly binds to and increases accessibility of Vgamma2 chromatin, thereby facilitating Vgamma2 rearrangement in the adult thymus. PMID: 21421735
    30. E2A binds to enhancer elements across the FOXO1 locus to activate Foxo1 expression, linking E2A and FOXO1 directly in a common pathway. PMID: 21972416
    31. transcriptional profiling of anterior forebrain precursors from mouse embryos expressing eGFP from the Hesx1 locus provides molecular evidence supporting a novel function of Hesx1 in mediating repression of Wnt/beta-catenin target activation. PMID: 22007134
    32. show how E2A promoted T lineage specification and how pre-TCR-mediated signaling affected E2A genome-wide occupancy PMID: 21857655
    33. T-cell factor 3 (Tcf3) deletion increases somatic cell reprogramming by inducing epigenome modifications. PMID: 21730189
    34. These data demonstrate that aberrant signalling by TCF/Lef1, transcription factors crucial for hair follicles bulge stem cell activation and hair differentiation, results in development of ectopic sebaceous glands originating from bulge cells. PMID: 21694721
    35. Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation. PMID: 21685889
    36. the interaction of bHLH transcription factor Hand2 with E2a is required for transcription of Phox2b, which is essential for autonomic nervous system neuron development. PMID: 21453680
    37. Data show that young and old primary B cells overexpressing a stable E47 mRNA up-regulate E47, AID, and CSR and improve B-cell immune responses in senescent murine B cells. PMID: 21241451
    38. A role for E47 in the tight control of hematopoietic stem cells proliferation and energy metabolism, and it is not required for short-term myeloid differentiation. PMID: 21273306
    39. HEBAlt interfered with the activity of E2A homodimers and with the expression of the transcription factor Pax5 PMID: 20826759
    40. PP2A dephosphorylation of p38 MAPK and/or TTP likely generates more binding of the hypophosphorylated TTP to the E47 mRNA, inducing its degradation. PMID: 20219523
    41. Results describe a new interaction between cell intrinsic aspects of the canonical Wnt/Tcf3/beta-catenin signaling pathway and extracellular Lif/Jak/Stat3 stimulation that combines to promote self-renewal and proliferation of embryonic stem cells. PMID: 20006604
    42. Scx and E47 might modulate the primary chondrogenesis by associating with the Sox9-related transcriptional complex, and by binding to the conserved E-box on Col2a1 promoter. PMID: 19828133
    43. Ca(2+) down-regulates SLC and CD19 gene expression upon pre-BCR activation through inhibition of E2A by Ca(2+)/calmodulin. PMID: 20022378
    44. Protein dimers containing complete basic helix-loop-helix domains but lacking other regions of E47 transcription factor have only 20% of the DNA binding ability and transactivation activity of wild-type dimers. PMID: 11820794
    45. When E2A gene disruption is allowed to occur exclusively throughout the double-negative stage of thymocyte development, the conditionally E2A-deficient mice do not develop T cell lymphoma. PMID: 11937548
    46. interacts with GRIPE, a transcription factor in developing forebrain PMID: 12200424
    47. E2a-H1f transcription factor plays a role in leukemic transformation of bone marrow B-cell progenitors PMID: 12370314
    48. evidence that E2A is directly involved in the transcriptional regulation of a number of early B-lineage genes PMID: 12415115
    49. E2A, along with histone acetyltransferases, regulates B cell development PMID: 12435739
    50. Levels of E2A protein expression in B cell precursors are stage-dependent and inhibited by stem cell factor, mainly from posttranscriptional regulation. PMID: 12482503

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: mmu:21423

    STRING: 10090.ENSMUSP00000100979

    UniGene: Mm.3406