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  4. Recombinant Rat F-box only protein 32 (Fbxo32)

Recombinant Rat F-box only protein 32 (Fbxo32)

  • 中文名称：
    大鼠Fbxo32重组蛋白
  • 货号：
    CSB-YP852812RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Fbxo32重组蛋白
  • 货号：
    CSB-EP852812RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Fbxo32重组蛋白
  • 货号：
    CSB-EP852812RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Fbxo32重组蛋白
  • 货号：
    CSB-BP852812RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Fbxo32重组蛋白
  • 货号：
    CSB-MP852812RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Fbxo32F-box only protein 32; Atrogin-1; Muscle atrophy F-box protein; MAFbx
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-350
  • 氨基酸序列
    MPFLGQDWRS PGQSWVKTAD GWKRFLDEKS GTFVSDLSSY CNKENLFNSL NYDVAAKKRK KDIQNSKTKT QYFHQEKWIY VHKGSTKERH GYCTLGEAFN RLDFSTAILD SRRFNYVVRL LELIAKSQLT SLSGIAQKNF MNILEKVVLK VLEDQQNIRL IRELLQTLYT SLCTLVQRVG KSVLVGNINM WVYRMETTLH WQQQLNSIQI SRPAFKGLTI TDLPVCLQLN IMQRLSDGRD LVSLGQAAPD LHVLSEDRLL WKRLCQYHFS ERQIRKRLIL SDKGQLDWKK MYFKLVRCYP RREQYGVTLQ LCKHCHILSW KGTDHPCTAN NPESCSVSLS PQDFINLFKF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
  • 基因功能参考文献：
    1. AMPK plays an important role in the inhibition of cardiomyocyte hypertrophy by activating protein degradation via FOXO3a/MAFbx signaling pathway. PMID: 27521792
    2. Suggest role for atrogin-1 up-regulation in simvastatin-induced heart mitochondria dysfunction. PMID: 25300705
    3. miR-19a/b family regulates phenotypes of cardiomyocytes via suppression of multiple direct target genes such as atrogin1. PMID: 24117217
    4. Co-expression of myocardin with atrogin-1 inhibited atrogin-1-induced myogenin expression. PMID: 23526547
    5. Overexpression of atrogin-1 results in marked alterations of gene expression profiles that are associated with cardiomyocyte survival, proliferation, inflammation, metabolism and hypertrophy. PMID: 23335977
    6. Resveratrol significantly inhibited PDGF-stimulated proliferation and cellular hypertrophy, which was also associated with improvements in atrogin-1 levels. PMID: 22146233
    7. Stability of F-box protein atrogin-1 is regulated by p38 mitogen-activated protein kinase pathway in cardiac H9c2 cells. PMID: 21691063
    8. In primary cultured rat cardiomyocytes, phenylephrine-induced activation of nuclear factor-kappaB and hypertrophy were significantly suppressed by MAFbx knockdown but were partially rescued by overexpression of nuclear factor-kappaB p65. PMID: 21617130
    9. FOXO3a mediates a reciprocal communication between the IRS-1/PI3K/Akt and IRS-2/MEK/ERK pathways that coordinates AT-1 and ubiquitin expression during muscle atrophy. PMID: 20371624
    10. Data suggest that the inhibitory effect of chronic arthritis on skeletal muscle is higher in fast glycolytic than in slow oxidative muscle and that IGF-I administration attenuates this effect and decreases atrogin-1 and IGFBP-3 gene expression. PMID: 20519361
    11. The high expression of MAFbx mRNA and protein, especially their persistent increases from 4 to 15 weeks after nerve reinnervation, is related to muscle atrophy and the decrease of muscle function. PMID: 19728616
    12. IGF-I inhibits atrogin-1/MAFbx expression PMID: 15284206
    13. Atrogin-1 is involved in the early stages of skeletal muscle regeneration in rats, independently of calcineurin. PMID: 15726428
    14. FBXO25 is a novel F-box protein analogous to atrogin-1, which is not involved in muscle atrophy. PMID: 16714087
    15. ES based on chronaxie values and applied to denervated muscles using surface electrodes was able to reduce atrogin-1 gene expression, which is related to muscular atrophy. PMID: 17034040
    16. MAFbx in muscle atrophy caused by blocked neuromuscular transmission and muscle unloading is described. PMID: 17622304
    17. Alcohol increased markers of oxidative stress, including atrogin-1, by approximately 60-fold, which was attenuated by procysteine supplementation. PMID: 17721978
    18. Alternate name for this locus: MAFbx. Alias: MAFbx. PMID: 11679633

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Specifically expressed in cardiac and skeletal muscle.
  • 数据库链接：

    KEGG: rno:171043

    STRING: 10116.ENSRNOP00000010361

    UniGene: Rn.72619