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  4. Recombinant Rat N (G),N (G)-dimethylarginine dimethylaminohydrolase 1 (Ddah1)

Recombinant Rat N (G),N (G)-dimethylarginine dimethylaminohydrolase 1 (Ddah1)

  • 中文名称：
    Recombinant Rat N(G),N(G)-dimethylarginine dimethylaminohydrolase 1(Ddah1),Yeast
  • 货号：
    CSB-YP006579RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat N(G),N(G)-dimethylarginine dimethylaminohydrolase 1(Ddah1),Yeast
  • 货号：
    CSB-EP006579RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat N(G),N(G)-dimethylarginine dimethylaminohydrolase 1(Ddah1),Yeast
  • 货号：
    CSB-EP006579RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat N(G),N(G)-dimethylarginine dimethylaminohydrolase 1(Ddah1),Yeast
  • 货号：
    CSB-BP006579RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat N(G),N(G)-dimethylarginine dimethylaminohydrolase 1(Ddah1),Yeast
  • 货号：
    CSB-MP006579RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ddah1
  • Uniprot No.：
  • 别名：
    Ddah1; DdahN(G),N(G)-dimethylarginine dimethylaminohydrolase 1; DDAH-1; Dimethylarginine dimethylaminohydrolase 1; EC 3.5.3.18; DDAHI; Dimethylargininase-1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-285
  • 氨基酸序列
    AGLSHPSVF GRATHAVVRA PPESLCRHAL RRSQGEEVDF ARAERQHQLY VGVLGSKLGL QVVQLPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK EVDMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG AEILADTFKD YAVSTVPVAD SLHLKSFCSM AGPNLIAIGS SESAQKALKI MQQMSDHRYD KLTVPDDMAA NCIYLNIPSK GHVLLHRTPE EYPESAKVYE KLKDHLLIPV SNSEMEKVDG LLTCCSVFIN KKTDS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • 基因功能参考文献：
    1. Data show that interleukin-10 (IL-10) attenuates angiotensin II-induced dimethylarginine dimethylaminohydrolase (DDAH-1) inhibition in spontaneously hypertensive rats vascular smooth muscle cells. PMID: 26375520
    2. after cholestasis an increase in hepatic ADMA in RL and ML was detected as well as tissue MMP-2 and MMP-9 activation in RL, supporting the evidence of functional heterogeneity among the liver lobes also occurring in an obstructive cholestasis model. PMID: 25013773
    3. The observed antihypertensive effects of NAC in young SHR might be due to restoration of DDAH activity to reduce ADMA, leading to attenuation of oxidative stress PMID: 24455716
    4. Pharmacological and genetic reduction of DDAH1 activity is protective against the vascular changes observed during endotoxic shock. PMID: 22995517
    5. DDAH1 is an important mediator of tumour progression, but appears to have addition roles independent of its metabolism of ADMA PMID: 21590769
    6. In healthy hearts, DDAH I is absent and DDAH II is localized to endothelium and endocardium, with a similar distribution to that of eNOS. The levels and activity of DDAH I and DDAH II are increased following myocardial infarction. PMID: 19481782
    7. dimethylarginine dimethylaminohydrolase plays a pivotal role in tumour growth and the development of the tumour vasculature by regulating the concentration of nitric oxide and altering vascular endothelial cell growth factor production. PMID: 12237779
    8. The DDAH1 mRNA expression in the embryonic spinal cord, which was especially strong in the ventral horn and dorsal root ganglion (DRG). DDAH1 was also detected in the brain, kidney, digestive tract, and in other tissues. PMID: 14766200
    9. Transfected into glioma cells in nude mice increases cell hypoxia and points to an inverse relationship of tumor oxygenation and angiogenesis in vivo. PMID: 15256062
    10. Decreased DDAH activity and elevated endogenous asymmetric dimethylarginine is implicated in endothelial dysfunction of rats exposed to glycosylated bovine serum albumin PMID: 16533509
    11. The DDAH activity and DDAH1, but not DDAH2, protein expression were significantly reduced in pulmonary artery endothelial cells of rats given monocrotaline PMID: 17322279
    12. Substitution of DDAH protein or enhancement of its activity may become a novel therapeutic strategy for the treatment of chronic kidney disease. PMID: 17409314
    13. asymmetric dimethylarginine is metabolized by DDAH-1, which is strongly in kidney cortex and liver. PMID: 17673667
    14. Kidney enzyme levels are increased in experimental diabetes. PMID: 17909098
    15. These findings suggest that the modulation of the DDAH/ADMA/ROS pathway plays an important role in CoCl(2)-induced apoptosis and the antiapoptotic effects of atRA in undifferentiated PC12 cells. PMID: 19156866
    16. Results suggest that alterations in DDAH-1/Nos inhibitors/NO-dependent cyclic GMP/ET-1 pathway are possibly involved in maintaining myometrial quiescence during gestation and controlling delivery at term. PMID: 19528264

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  • 蛋白家族：
    DDAH family
  • 组织特异性：
    Detected in red blood cells (at protein level). Widely distributed, high amounts found in kidney, brain, aorta and pancreas.
  • 数据库链接：

    KEGG: rno:64157

    STRING: 10116.ENSRNOP00000044971

    UniGene: Rn.7398