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  4. Recombinant Rat Pyruvate kinase PKLR (Pklr)

Recombinant Rat Pyruvate kinase PKLR (Pklr)

  • 货号：
    CSB-BP018071RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-EP018071RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-MP018071RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Name:Pklr
  • Uniprot No.：
  • 别名：
    PklrPyruvate kinase PKLR; EC 2.7.1.40; L-PK; Pyruvate kinase isozymes L/R
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-574
  • 氨基酸序列
    MSVQENTLPQ QLWPWIFRSQ KDLAKSALSG APGGPAGYLR RASVAQLTQE LGTAFFQQQQ LPAAMADTFL EHLCLLDIDS QPVAARSTSI IATIGPASRS VDRLKEMIKA GMNIARLNFS HGSHEYHAES IANIREATES FATSPLSYRP VAIALDTKGP EIRTGVLQGG PESEVEIVKG SQVLVTVDPK FQTRGDAKTV WVDYHNITRV VAVGGRIYID DGLISLVVQK IGPEGLVTEV EHGGILGSRK GVNLPNTEVD LPGLSEQDLL DLRFGVQHNV DIIFASFVRK ASDVLAVRDA LGPEGQNIKI ISKIENHEGV KKFDEILEVS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NLAGKPVVCA TQMLESMITK ARPTRAETSD VANAVLDGAD CIMLSGETAK GSFPVEAVMM QHAIAREAEA AVYHRQLFEE LRRAAPLSRD PTEVTAIGAV EASFKCCAAA IIVLTKTGRS AQLLSQYRPR AAVIAVTRSA QAARQVHLSR GVFPLLYREP PEAIWADDVD RRVQFGIESG KLRGFLRVGD LVIVVTGWRP GSGYTNIMRV LSVS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a key role in glycolysis.
  • 基因功能参考文献：
    1. Studied the effect of the point mutations around this phosphorylation site on the catalytic properties of L-pyruvate kinase. PMID: 22878931
    2. c-Myc is required for the glucose-mediated induction of this metabolic enzyme. PMID: 12480946
    3. We observed that cystine inhibited the enzyme pyruvate kinase activity non-competitively in a dose- and time-dependent way. PMID: 15196592
    4. cooperative interaction between NF1 family members and HNF1alpha plays an important role in hepatic L-PK transcription PMID: 16204235
    5. Regulation of hepatic L-pyruvate kinase promoter composition and activity PMID: 16644726
    6. A novel chimeric promoter, consisting of a 1.6 kb glucose 6-phosphatase promoter and a segment of the regulatory element derived from the L-type pyruvate kinase promoter, was designed to provide strong and tight control of insulin expression in liver. PMID: 17160355
    7. Maximal glucose-induced expression of the L-PK gene in INS-1-derived 832/13 cells involves increased c-Myc abundance, recruitment of c-Myc, Max, and ChREBP to the promoter, and a glucose-stimulated increase in ChREBP transactivation. PMID: 17341548
    8. A detailed analysis of the molecular events of the insulin-independent glucose response of the liver-type pyruvate kinase (L-PK) gene was performed. PMID: 18468514
    9. cAMP and glucose signaling converge on a complex containing ChREBP, HNF4alpha, and CBP, and cAMP acts by disrupting this transcriptional complex assembled by glucose-derived signals PMID: 19406844
    10. Data show that glucose regulates acetylation and methylation of various histone residues at the L-PK gene promoter. PMID: 19631660

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  • 蛋白家族：
    Pyruvate kinase family
  • 数据库链接：

    KEGG: rno:24651

    STRING: 10116.ENSRNOP00000027700

    UniGene: Rn.48821