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  4. Recombinant Rat Tribbles homolog 3 (Trib3)

Recombinant Rat Tribbles homolog 3 (Trib3)

  • 中文名称：
    大鼠Trib3重组蛋白
  • 货号：
    CSB-YP892975RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Trib3重组蛋白
  • 货号：
    CSB-EP892975RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Trib3重组蛋白
  • 货号：
    CSB-EP892975RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Trib3重组蛋白
  • 货号：
    CSB-BP892975RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Trib3重组蛋白
  • 货号：
    CSB-MP892975RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Trib3; Nipk; Tribbles homolog 3; TRB-3; Neuronal cell death-inducible putative kinase
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-349
  • 氨基酸序列
    MRATSLAASA DVPCRKKPLE FDDNIDVECP VLKRVRDEPE PGPTPSLPPA SDLSPAVAPA TRLGPYILLE REQGNCTYRA LHCPTGTEYT CKVYPASEAQ AVLAPYARLP THQHVARPTE VLLGSQLLYT FFTKTHGDLH SLVRSRRGIP EPEAAALFRQ MASAVAHCHK HGLILRDLKL RRFVFSNCER TKLVLENLED ACVMTGPDDS LWDKHACPAY VGPEILSSRP SYSGRAADVW SLGVALFTML AGRYPFQDSE PALLFGKIRR GTFALPEGLS ASARCLIRCL LRREPSERLV ALGILLHPWL REDCSQVSPP RSDRREMDQV VPDGPQLEEA EEGEVGLYG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity. Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. Can inhibit APOBEC3A editing of nuclear DNA.
  • 基因功能参考文献：
    1. TRB3 participated in the global cerebral ischemia and reperfusion injury-induced neuronal apoptosis PMID: 28782643
    2. Silencing of TRB3 has a significant ameliorative effect on diabetic nephropathy in rats. PMID: 28600485
    3. data suggest that TRB3 mediates AGE-induced oxidative injury in beta-cells through the PKCbeta2 pathway. PMID: 28534945
    4. The TRB3 silencing alleviated adipocytes insulin resistance. With TRB3 gene silencing, protein levels of IRS-1, GLUT4 and p-AMPK were significantly increased in adipocytes. TRB3 gene silencing decreased blood glucose, ameliorated insulin sensitivity and adipose tissue remodeling in diabetic rats. TRB3 silencing decreased triglyceride, increased glycogen simultaneously in diabetic epididymal and brown adipose tissues (BAT) PMID: 28476619
    5. Trib3 may be associated with the pathogenic mechanism of demyelination in dmy rats. PMID: 27977799
    6. results suggest that a self-amplifying feed-forward loop among Trib3, Akt, and FoxO1 in Abeta-treated neurons induces both apoptosis and autophagy, culminating in neuron death PMID: 28011637
    7. Diabetic rats displayed increased aortic medial thickness, excessive collagen deposition, diminished elastic fibres and reduced vascular compliance with Tribbles 3 overexpression. Trib3 silencing reversed pathological aortic remodeling in diabetic rats. PMID: 26410836
    8. Findings suggest a role for TRIB3 in the development of acute insulin resistance following injury PMID: 26818585
    9. TRB3 plays an important role in stretch-induced cardiomyocyte apoptosis. PMID: 25898323
    10. Taken together, these results suggest that TRB3 mediates renal tubular cell apoptosis induced by protein overload, broadening our understanding of the pathogenesis of progressive proteinuric kidney diseases. PMID: 24925634
    11. Trib3 interferes with the Parkinson's disease-associated prosurvival protein Parkin to mediate death PMID: 26224857
    12. Upregulated TRIB3 expression could induce renal fibrosis accompanied by increased phosphorylation of extracellular signal-regulated kinase. PMID: 24368111
    13. knockdown of TRIB3 inhibited, and TRIB3 overexpression enhanced, the ability of both high glucose and glucosamine to induce insulin resistance. PMID: 23990361
    14. TRB3 may serve as a negative regulator of pro-inflammatory cytokines and chemokines, controlling the extent of the inflammatory response. PMID: 23000510
    15. It was shown that TRIB3 impacts glucose uptake and oxidation oppositely in muscle and fat. The data implicated TRIB3 for the first time as a potent physiological regulator of insulin sensitivity and mitochondrial glucose oxidation. PMID: 22850683
    16. investigation into whether TRB3 plays a major role in diabetic cardiomyopathy (DCM) and the mechanisms involved: TRB3 gene silencing may exert a protective effect on DCM by improving selective insulin resistance. PMID: 21933987
    17. CHOP and ATF4 might mediate PA-induced TRIB3 expression and, by extension, the suppression of IL6 activation of pro-survival kinase AKT. PMID: 20488947
    18. These data identify TRIB3 induction as a novel molecular mechanism in human insulin resistance and diabetes. PMID: 19996382
    19. The present study does not support the hypothesis that the physiological role of TRB3/NIPK might be to put a brake on insulin signalling in hepatocytes. PMID: 15469416
    20. TRB3 has a role in inhibition of Akt/protein kinase B activation PMID: 16452480
    21. TRB3 may be involved in metabolic syndrome by inhibiting activation of Akt in adipose tissue. PMID: 18497449
    22. Data demonstrated that overexpression of TRIB3 mimicked the glucotoxic effects on insulin secretion and cell growth in INS1 cells. Induction of TRIB3 synergistically enhanced high-glucose-elicited apoptosis. PMID: 18818302
    23. blocks insulin signaling by inhibiting Akt activation, which contributes to insulin resistance; may be an important factor in the occurrence and development of fatty liver PMID: 19452573

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, Tribbles subfamily
  • 组织特异性：
    Detected only in the lung. Not detected in the heart, brain, spleen, liver, skeletal muscle, kidney and testis.
  • 数据库链接：

    KEGG: rno:246273

    STRING: 10116.ENSRNOP00000009840

    UniGene: Rn.22325