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  4. Recombinant Saccharomyces cerevisiae Diphthamide biosynthesis protein RRT2 (RRT2)

Recombinant Saccharomyces cerevisiae Diphthamide biosynthesis protein RRT2 (RRT2)

  • 中文名称：
    酿酒酵母RRT2重组蛋白
  • 货号：
    CSB-YP330864SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母RRT2重组蛋白
  • 货号：
    CSB-EP330864SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母RRT2重组蛋白
  • 货号：
    CSB-EP330864SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母RRT2重组蛋白
  • 货号：
    CSB-BP330864SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母RRT2重组蛋白
  • 货号：
    CSB-MP330864SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RRT2
  • Uniprot No.：
  • 别名：
    RRT2; DPH7; ERE1; YBR246W; YBR1634Diphthine methyltransferase; EC 3.1.1.97; Diphthamide biosynthesis protein 7; Endosomal recycling protein 1; Regulator of rDNA transcription protein 2
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-387
  • 氨基酸序列
    MDSIQESDVL NAVKTKLPPC CLRIFRNKII LVGTYDLDKS TGYRSGSLDV FTMDLKLLCS NNTYGAILDL KLSPFDDTLI CTAHSTGNIM LWRIRCTDKD DFQSNELDIH AIANLQLFEK DVLIASCHFS PLDCKKLLVT NTAGEAATID IRTLSVQFTA SAIAQAYSKL DKIDYEVQGA TEKVIHVESG QFLKPHELEC WTAEFGSLQP FQDVVFTGGD DSRIMAHDLR SKEFIWSNNR IHDAGVVSIK CSQPNFRNNK PTSIITGSYD DNIRSLDLRM MGESIFPGAN VPTVNKLACD LGGGVWRFVE SPIDQEQSHH NGSDRLLVCC MYNGAKVVTM NDNSDEYFQI QHYLKKGHDS MCYGGDWSNS LIATCSFYDN SLQTWIV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the demethylation of diphthine methyl ester to form diphthine, an intermediate in diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 (EFT1 and EFT2). Also plays a role in the regulation of the retromer complex and is required for the recycling from endosomes of plasma membrane proteins like CAN1 and MUP1. Identified in a screen for mutants with decreased levels of rDNA transcription.
  • 基因功能参考文献：
    1. Dph5-catalyzed reaction generates methylated diphthine, a previously overlooked intermediate, and Dph7 is a methylesterase that hydrolyzes methylated diphthine to produce diphthine and allows the Dph6-catalyzed amidation reaction to occur. PMID: 24739148
    2. It was shown that the YBR246W gene in yeast is required for the last step of diphthamide biosynthesis, as the deletion of YBR246W leads to the accumulation of diphthine, which is the enzymatic product of the second step of the biosynthesis. PMID: 22188241
    3. our data indicate that a complex containing the two novel proteins, Ere1 and Ere2, mediates cargo-specific recognition by the retromer pathway. [Ere1] PMID: 21880895
  • 亚细胞定位：
    Cytoplasm. Endosome. Note=Recruited to endosomes in cells in which increased recycling of internalized plasma membrane proteins occurs.
  • 蛋白家族：
    DPH7 family
  • 数据库链接：

    KEGG: sce:YBR246W

    STRING: 4932.YBR246W