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  4. Recombinant Saccharomyces cerevisiae GCR1-dependent translation factor 1 (GDT1), partial

Recombinant Saccharomyces cerevisiae GCR1-dependent translation factor 1 (GDT1), partial

  • 中文名称：
    酿酒酵母GDT1重组蛋白
  • 货号：
    CSB-YP336432SVG1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母GDT1重组蛋白
  • 货号：
    CSB-EP336432SVG1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母GDT1重组蛋白
  • 货号：
    CSB-EP336432SVG1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母GDT1重组蛋白
  • 货号：
    CSB-BP336432SVG1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母GDT1重组蛋白
  • 货号：
    CSB-MP336432SVG1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GDT1
  • Uniprot No.：
  • 别名：
    GDT1; YBR187W; YBR1310; GCR1-dependent translation factor 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be involved in ribosome biogenesis. Has also been proposed to play a role in calcium homeostasis.
  • 基因功能参考文献：
    1. Gdt1p,to sustain the Golgi glycosylation process, imports Mn(2+) inside the Golgi lumen when Pmr1p exclusively transports Ca(2+). Gdt1p is a Mn(2+)-sensitive protein whose abundance dependents on the kind of the ion transported by Pmr1p. The aspartic residues of the 2 conserved motifs E-x-G-D-[KR], in the cation binding sites of Gdt1p, play a role in Golgi glycosylation and hence in Mn(2+)/Ca(2+)transport. PMID: 29108953
    2. Gdt1p controls cellular calcium stores and plays a major role in the calcium response induced by osmotic shock when the Golgi calcium pump, Pmr1p, is absent. Furthermore, in the presence of a high concentration of external calcium, Gdt1p is required for glycosylation of carboxypeptidase Y and the glucanosyltransferase Gas1p. PMID: 27075443
  • 亚细胞定位：
    Vacuole membrane; Multi-pass membrane protein. Golgi apparatus membrane.
  • 蛋白家族：
    GDT1 family
  • 数据库链接：

    KEGG: sce:YBR187W

    STRING: 4932.YBR187W