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  4. Recombinant Saccharomyces cerevisiae N-lysine methyltransferase SEE1 (SEE1)

Recombinant Saccharomyces cerevisiae N-lysine methyltransferase SEE1 (SEE1)

  • 中文名称：
    酿酒酵母EFM4重组蛋白
  • 货号：
    CSB-YP336734SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母EFM4重组蛋白
  • 货号：
    CSB-EP336734SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母EFM4重组蛋白
  • 货号：
    CSB-EP336734SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母EFM4重组蛋白
  • 货号：
    CSB-BP336734SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母EFM4重组蛋白
  • 货号：
    CSB-MP336734SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EFM4
  • Uniprot No.：
  • 别名：
    EFM4; SEE1; YIL064WProtein-lysine N-methyltransferase EFM4; EC 2.1.1.-; Elongation factor methyltransferase 4; Secretion and early endocytosis protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-257
  • 氨基酸序列
    MKRSEKKSMS SALKNGIMER TQPEKVVQMQ GTADLSTSKL GTKKYWDELY ALELENFRRN PQDTGDCWFS DSDAEQKMID FLVDNIGAYR ISENASVVDL GTGNGHMLFE LHQTEFQGKL VGIDYSEESV KLASNIAEAT GVDNFISFQQ ADIFSGDWKP GKYDIVLDKG TLDAISLSGM KINGKLDVVD VYAGVVERIL KKDGIFLITS CNFTQDELVK IIETDNLKMW KTIKYPVFQF GGVQGATICS VAFVKQN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that mono- and dimethylates elongation factor 1-alpha (TEF1 and TEF2) at 'Lys-316'. May play a role in intracellular transport.
  • 基因功能参考文献：
    1. Methylation of TEF1 by See1 facilitates tombusvirus replication in Saccharomyces cerevisiae. PMID: 24314635
    2. YHL039W (now designated EFM1 for elongation factor methyltransferase 1) and YIL064W/SEE1 encode distinct eEF1A methyltransferases that respectively monomethylate and dimethylate this protein at lysine residues PMID: 20510667
    3. These are the first in vivo data reporting a functional role for the protein encoded by ORF YIL064w and identify Yil064p, named here secretion and early endocytosis 1 protein (See1p), as a novel component of intracellular transport. PMID: 19160456
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, EFM4 family
  • 数据库链接：

    KEGG: sce:YIL064W

    STRING: 4932.YIL064W