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  4. Recombinant Saccharomyces cerevisiae Peroxiredoxin HYR1 (HYR1)

Recombinant Saccharomyces cerevisiae Peroxiredoxin HYR1 (HYR1)

  • 中文名称：
    酿酒酵母HYR1重组蛋白
  • 货号：
    CSB-YP328140SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母HYR1重组蛋白
  • 货号：
    CSB-EP328140SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母HYR1重组蛋白
  • 货号：
    CSB-EP328140SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母HYR1重组蛋白
  • 货号：
    CSB-BP328140SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母HYR1重组蛋白
  • 货号：
    CSB-MP328140SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HYR1
  • Uniprot No.：
  • 别名：
    HYR1; GPX3; ORP1; YIR037WGlutathione peroxidase-like peroxiredoxin HYR1; EC 1.11.1.15; Glutathione peroxidase homolog 3; GPx 3; Hydrogen peroxide resistance protein 1; Oxidant receptor peroxidase 1; Phospholipid hydroperoxide glutathione peroxidase 3; PHGPx3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-163
  • 氨基酸序列
    MSEFYKLAPV DKKGQPFPFD QLKGKVVLIV NVASKCGFTP QYKELEALYK RYKDEGFTII GFPCNQFGHQ EPGSDEEIAQ FCQLNYGVTF PIMKKIDVNG GNEDPVYKFL KSQKSGMLGL RGIKWNFEKF LVDKKGKVYE RYSSLTKPSS LSETIEELLK EVE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in oxidative stress response and redox homeostasis. Functions as a sensor and transducer of hydroperoxide stress. In response to hydroperoxide stress it oxidizes (activates) the transcription activator YAP1, which is involved in transcription activation of genes of the oxidative stress response pathway. May also play a direct role in hydroperoxide scavenging, being the most active of three closely related S.cerevisiae peroxiredoxins (GPX1, GPX2, and HYR1/GPX3) with respect to peroxide and lipid hydroperoxide reduction. The three enzymes are not required for the glutaredoxin-mediated antioxidant function. In the presence of peroxides, HYR1/GPX3 is directly oxidized at Cys-36 to form a cysteine sulfenic acid (-SOH). Cys-36-SOH then forms either an intramolecular disulfide bond (Cys-36 with Cys-82) or a transient, intermolecular disulfide bond with 'Cys-598' of YAP1, which is further resolved into a YAP1 intramolecular disulfide bond ('Cys-303' with 'Cys-598'), which causes its nuclear accumulation and activation, and a reduced Cys-36 in HYR1/GPX3.
  • 基因功能参考文献：
    1. Our data suggested that gpx3 is linked with maintenance of cellular phospholipids during Cd-induced oxidative stress. The results indicate that gpx3 might protect the phospholipids from Cd-induced oxidative damage in Saccharomyces cerevisiae. PMID: 21229313
    2. glutathione peroxidase 3 protects GAPDH from NO stress and thereby contributes to the maintenance of homeostasis during exposure to NO stress PMID: 21229323
    3. phospholipid hydroperoxidase and Yap1-mediated signaling activities of Gpx3 have independent functional roles PMID: 15337745
    4. Gpx3 functions as a redox-dependent exquisite regulator of the protein repair activity of methionine sulfoxide reductase Mxr1 PMID: 16808898
    5. Data suggested that Gpx3 protects GS from non-enzymatic proteolysis, thereby contributing to cell homeostasis when cell is exposed to oxidative stress. PMID: 17707771
    6. Molecular mechanism of oxidative stress perception by ORP1 is reported. PMID: 17720812
    7. Study identified 30 proteins that are related to the Gpx3-dependent oxidative stress responses and 17 proteins that are changed in a Gpx3-dependent manner regardless of oxidative stress. PMID: 18309271
    8. The crystal structure of Hyr1 from Saccharomyces cerevisiae, is reported. PMID: 18767166
    9. Gpx3 interacts with Dak1, alleviates dihydroxyacetone-mediated stress by upregulating Dak activity, and consequently suppresses advanced glycation endproducts formation. PMID: 19715675

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  • 亚细胞定位：
    Cytoplasm. Mitochondrion intermembrane space. Peroxisome matrix.
  • 蛋白家族：
    Glutathione peroxidase family
  • 数据库链接：

    KEGG: sce:YIR037W

    STRING: 4932.YIR037W