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  4. Recombinant Saccharomyces cerevisiae Sphingolipid long chain base-responsive protein PIL1 (PIL1)

Recombinant Saccharomyces cerevisiae Sphingolipid long chain base-responsive protein PIL1 (PIL1)

  • 中文名称：
    酿酒酵母PIL1重组蛋白
  • 货号：
    CSB-YP346841SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母PIL1重组蛋白
  • 货号：
    CSB-EP346841SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母PIL1重组蛋白
  • 货号：
    CSB-EP346841SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母PIL1重组蛋白
  • 货号：
    CSB-BP346841SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母PIL1重组蛋白
  • 货号：
    CSB-MP346841SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PIL1
  • Uniprot No.：
  • 别名：
    PIL1; YGR086C; Sphingolipid long chain base-responsive protein PIL1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-339
  • 氨基酸序列
    MHRTYSLRNS RAPTASQLQN PPPPPSTTKG RFFGKGGLAY SFRRSAAGAF GPELSRKLSQ LVKIEKNVLR SMELTANERR DAAKQLSIWG LENDDDVSDI TDKLGVLIYE VSELDDQFID RYDQYRLTLK SIRDIEGSVQ PSRDRKDKIT DKIAYLKYKD PQSPKIEVLE QELVRAEAES LVAEAQLSNI TRSKLRAAFN YQFDSIIEHS EKIALIAGYG KALLELLDDS PVTPGETRPA YDGYEASKQI IIDAESALNE WTLDSAQVKP TLSFKQDYED FEPEEGEEEE EEDGQGRWSE DEQEDGQIEE PEQEEEGAVE EHEQVGHQQS ESLPQQTTA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Negative regulator of cell wall integrity (CWI) in unstressed cells, probably by inhibiting protein kinase PKH1/PHK2 activity and regulating their downstream CWI pathways PKC1-MAP kinase pathway and protein kinase YPK1 pathway. Activity may be regulated by the transient increase of sphingolipid long chain bases (LCBs) during heat stress.
  • 基因功能参考文献：
    1. Seg1/Sle1 controls eisosome assembly and shape, which also involves Pil1 and Lsp1 PMID: 22869600
    2. Pil1 and Lsp1 self-assemble into higher-order structures and bind preferentially to phosphoinositide-containing membranes. PMID: 22123866
    3. Pil1, an eisosome organizer, plays an important role in the recruitment of synaptojanins and amphiphysins to facilitate receptor-mediated endocytosis in yeast PMID: 21872358
    4. Loss of Pil1 leads to clustering of eisosome remnants and redirects endocytosis and endocytic effector proteins to these clusters PMID: 16496001
    5. Pil1 expression is regulated by the cell cycle, which synchronizes eisosome formation with plasma membrane growth. PMID: 19037108

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  • 亚细胞定位：
    Lipid droplet.
  • 数据库链接：

    KEGG: sce:YGR086C

    STRING: 4932.YGR086C