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  4. Recombinant Saccharomyces cerevisiae Ubiquitin-like protein SMT3 (SMT3)

Recombinant Saccharomyces cerevisiae Ubiquitin-like protein SMT3 (SMT3)

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  • 中文名称：
    酿酒酵母SMT3重组蛋白
  • 货号：
    CSB-EP615489SVG
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    SMT3
  • Uniprot No.：
  • 别名：
    DmSUMO 1; Small Ubiquitin-like modifier; SMT3; SMT3_YEAST; Ubiquitin like protein of the SUMO family; Ubiquitin like protein SMT3; Ubiquitin-like protein SMT3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    27.1kDa
  • 表达区域：
    2-98aa
  • 氨基酸序列
    SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The recombinant Baker's yeast SMT3 protein is encoded by the gene of SMT3 (2-98aa). The gene of SMT3 was cloned in a system (E.coli) that supported the expression of SMT3 and translation of messenger RNA. Modification of SMT3 by recombinant DNA technology could lead to the expression of the target protein. The protein was fused with N-terminal 6xHis-SUMO tag in the production. The purity is 90% determined by SDS-PAGE.

    SMT3 is a protein coding gene that encodes Ubiquitin-like protein SMT3. According to some studies, SMT3 may have the following features.
    A new factor required for the SUMO1/Smt3 conjugation of yeast membrane proteins. The ubiquitin-like proteins SMT3 and SUMO-1 are bound by the UBC9 E2 enzyme. Yeast ULP2 (SMT4) gene encodes a new protease, which is specific for ubiquitin-like Smt3 protein. Yeast Ull1/Siz1 is a new type of SUMO1/Smt3 ligase, used for septin components, and acts as an adaptor between the ligase and the substrate. A new type of mammalian Smt3-specific isopeptidase 1 (SMT3IP1) localizes to nucleosomes during interphase. Sterol methyltransferases SMT1, SMT2 and SMT3 affect the development of Arabidopsis thaliana through non-sterol products. The combination of Smt3 and Dorsal may enhance the immune response of fruit flies.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Not known; suppressor of MIF2 mutations.
  • 基因功能参考文献：
    1. the library of Smt3 mutants represents a valuable resource for further exploring the functions of sumoylation in cellular stress response and other SUMO-dependent pathways. PMID: 28166236
    2. Ulp2 controls the dynamic range of SUMO chain lengths by trimming them from the distal ends PMID: 25833950
    3. Cdk1 and SUMO (Smt3) regulate Swe1 stability PMID: 21151918
    4. the Zn(2+) motif of E1 has a role in SUMO adenylation PMID: 20501649
    5. there are a broad array of cellular processes regulated by SUMO conjugation PMID: 15590687
    6. A model is proposed for the positive control of the centromeric pool of Top2p, required for high minichromosome segregation fidelity, by Smt3p modification. PMID: 16204216

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  • 蛋白家族：
    Ubiquitin family, SUMO subfamily
  • 数据库链接：

    KEGG: sce:YDR510W

    STRING: 4932.YDR510W