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  4. Recombinant Saccharomyces cerevisiae Ubiquitin-like protein SMT3 (SMT3)

Recombinant Saccharomyces cerevisiae Ubiquitin-like protein SMT3 (SMT3)

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  • 中文名称：
    酿酒酵母SMT3重组蛋白
  • 货号：
    CSB-YP615489SVG
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    SMT3
  • Uniprot No.：
  • 别名：
    DmSUMO 1; Small Ubiquitin-like modifier; SMT3; SMT3_YEAST; Ubiquitin like protein of the SUMO family; Ubiquitin like protein SMT3; Ubiquitin-like protein SMT3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    13.1kDa
  • 表达区域：
    2-98aa
  • 氨基酸序列
    SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Ubiquitin-like protein SMT3 CSB-YP615489SVG is a recombinant Saccharomyces cerevisiae full-length of mature SMT3 protein containing amino acid residues of 2-98. It is produced in the yeast cells through Eukaryotic expression and fused with a 6xHis-tag at the N-terminus. And it has high purity of up to 90% determined by SDS-PAGE. Its predicted molecular weight is 13.1 kDa, but the actual observed molecular mass via SDS-PAGE analysis is slightly higher due to post-transcriptional modifications such as glycosylation. This recombinant SMT3 protein is in-stock, allowing for continuous sourcing and eliminating the intermediate waiting periods for protein preparation. This SMT3 protein not only acts as an immunogen for antibody production but also finds uses in the studies of microbiology.

    SMT3 is the only small ubiquitin-like modifier (SUMO) protein of Saccharomyces cerevisiae. Like ubiquitin, SMT3 consists of multiple lysine residues that could serve as sites for synthesis of topologically and functionally distinct chains. TongLiu etc. demonstrated that SMT3 plays critical roles in the asexual development, environmental adaptation, and pathogenicity of Beauveria bassiana.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Not known; suppressor of MIF2 mutations.
  • 基因功能参考文献：
    1. the library of Smt3 mutants represents a valuable resource for further exploring the functions of sumoylation in cellular stress response and other SUMO-dependent pathways. PMID: 28166236
    2. Ulp2 controls the dynamic range of SUMO chain lengths by trimming them from the distal ends PMID: 25833950
    3. Cdk1 and SUMO (Smt3) regulate Swe1 stability PMID: 21151918
    4. the Zn(2+) motif of E1 has a role in SUMO adenylation PMID: 20501649
    5. there are a broad array of cellular processes regulated by SUMO conjugation PMID: 15590687
    6. A model is proposed for the positive control of the centromeric pool of Top2p, required for high minichromosome segregation fidelity, by Smt3p modification. PMID: 16204216

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  • 蛋白家族：
    Ubiquitin family, SUMO subfamily
  • 数据库链接：

    KEGG: sce:YDR510W

    STRING: 4932.YDR510W