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  4. Recombinant Saccharomyces cerevisiae Vacuolar-sorting protein SNF7 (SNF7)

Recombinant Saccharomyces cerevisiae Vacuolar-sorting protein SNF7 (SNF7)

  • 中文名称：
    酿酒酵母SNF7重组蛋白
  • 货号：
    CSB-YP328049SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母SNF7重组蛋白
  • 货号：
    CSB-EP328049SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母SNF7重组蛋白
  • 货号：
    CSB-EP328049SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母SNF7重组蛋白
  • 货号：
    CSB-BP328049SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母SNF7重组蛋白
  • 货号：
    CSB-MP328049SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SNF7
  • Uniprot No.：
  • 别名：
    SNF7; DID1; VPS32; YLR025WVacuolar-sorting protein SNF7; DOA4-independent degradation protein 1; Sucrose nonfermenting protein 7; Vacuolar protein-sorting-associated protein 32
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-240
  • 氨基酸序列
    MWSSLFGWTS SNAKNKESPT KAIVRLREHI NLLSKKQSHL RTQITNQENE ARIFLTKGNK VMAKNALKKK KTIEQLLSKV EGTMESMEQQ LFSIESANLN LETMRAMQEG AKAMKTIHSG LDIDKVDETM DEIREQVELG DEISDAISRP LITGANEVDE DELDEELDML AQENANQETS KIVNNNVNAA PISENKVSLP SVPSNKIKQS ENSVKDGEEE EDEEDEDEKA LRELQAEMGL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts a component of the ESCRT-III complex required for the sorting and concentration of proteins resulting in the entry of these proteins into the invaginating vesicles of the multivesicular body (MVB). The sequential action of ESCRT-0, -I, and -II together with the ordered assembly of ESCRT-III links membrane invagination to cargo sorting. Membrane scission in the neck of the growing vesicle releases mature, cargo-laden ILVs into the lumen. ESCRT-III is critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. SNF7 is the most abundant ESCRT-III subunit which forms membrane-sculpting filaments with 30 Angstrom periodicity and a exposed cationic membrane-binding surface. Its activation requires a prominent conformational rearrangement to expose protein-membrane and protein-protein interfaces. SNF7 filaments then form spirals that could function as spiral springs. The elastic expansion of compressed SNF7 spirals generates an area difference between the two sides of the membrane and thus curvature which could be the origin of membrane deformation leading eventually to fission. SNF7 recruits BRO1, which in turn recruits DOA4, which deubiquitinates cargos before their enclosure within MVB vesicles. ESCRT-III is also recruited to the nuclear envelope (NE) by integral INM proteins to surveil and clear defective nuclear pore complex (NPC) assembly intermediates to ensure the fidelity of NPC assembly.
  • 基因功能参考文献：
    1. The major ESCRT-III subunit Snf7 localizes transiently to vesicles and tubules surrounded by a cup-shaped membrane and this was accelerated in vps4Delta cells, correlating with increased Acb1 secretion. PMID: 27115345
    2. Novel mutations has been characterized in ESCRT-III Snf7 that trigger activation, and a novel role have been assigned to Bro1, the yeast ALIX ortholog, in Snf7 assembly. PMID: 27074665
    3. Using a combination of biochemical and genetic approaches, both in vitro and in vivo, we demonstrate that mutations on these protein interfaces halt Snf7 assembly and block endosomal sorting complexes required for transport function. PMID: 26670543
    4. Snf7 alpha6 helix is crucial for binding of Bro1 and Rim20 in vivo and is indispensable for the multivesicular body sorting. PMID: 24058170
    5. Binding of Vps4 to ESCRT-III, coordinated by interactions with Vps2 and Snf7, is coupled to membrane neck constriction during intraluminal vesicle formation. PMID: 24711499
    6. we demonstrate that the N terminus of Snf7 has been tuned to maintain the topological constraints associated with ESCRT-III-filament-mediated membrane invagination and vesicle formation. PMID: 24139821
    7. A rns4/vps32 mutation on the RNase T1 expression-sensitive strain of Saccharomyces Cerevisiae provides evidence for altered ambient response resulting in transportation of the secretory protein to vacuoles. PMID: 15925308
    8. Snf7 influences SUC2 gene expression through activation of the Rim101 pathway. PMID: 18806212
    9. By generating SNF7 mutations that severely affect endocytic trafficking, but leave the Rim pathway function intact, data show that the two functions of SNF7 can be separated genetically. PMID: 19725809

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  • 亚细胞定位：
    Cytoplasm. Endosome membrane; Peripheral membrane protein. Nucleus envelope.
  • 蛋白家族：
    SNF7 family
  • 数据库链接：

    KEGG: sce:YLR025W

    STRING: 4932.YLR025W