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  4. Recombinant Saccharomyces cerevisiae mRNA-capping enzyme subunit alpha (CEG1)

Recombinant Saccharomyces cerevisiae mRNA-capping enzyme subunit alpha (CEG1)

  • 中文名称：
    Recombinant Saccharomyces cerevisiae mRNA-capping enzyme subunit alpha(CEG1),Yeast
  • 货号：
    CSB-YP311511SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae mRNA-capping enzyme subunit alpha(CEG1),Yeast
  • 货号：
    CSB-EP311511SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae mRNA-capping enzyme subunit alpha(CEG1),Yeast
  • 货号：
    CSB-EP311511SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae mRNA-capping enzyme subunit alpha(CEG1),Yeast
  • 货号：
    CSB-BP311511SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae mRNA-capping enzyme subunit alpha(CEG1),Yeast
  • 货号：
    CSB-MP311511SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CEG1
  • Uniprot No.：
  • 别名：
    CEG1; YGL130W; G2853; mRNA-capping enzyme subunit alpha; GTP--RNA guanylyltransferase; GTase; mRNA guanylyltransferase; EC 2.7.7.50
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-459
  • 氨基酸序列
    MVLAMESRVA PEIPGLIQPG NVTQDLKMMV CKLLNSPKPT KTFPGSQPVS FQHSDVEEKL LAHDYYVCEK TDGLRVLMFI VINPVTGEQG CFMIDRENNY YLVNGFRFPR LPQKKKEELL ETLQDGTLLD GELVIQTNPM TKLQELRYLM FDCLAINGRC LTQSPTSSRL AHLGKEFFKP YFDLRAAYPN RCTTFPFKIS MKHMDFSYQL VKVAKSLDKL PHLSDGLIFT PVKAPYTAGG KDSLLLKWKP EQENTVDFKL ILDIPMVEDP SLPKDDRNRW YYNYDVKPVF SLYVWQGGAD VNSRLKHFDQ PFDRKEFEIL ERTYRKFAEL SVSDEEWQNL KNLEQPLNGR IVECAKNQET GAWEMLRFRD DKLNGNHTSV VQKVLESIND SVSLEDLEEI VGDIKRCWDE RRANMAGGSG RPLPSQSQNA TLSTSKPVHS QPPSNDKEPK YVDEDDWSD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate.
  • 基因功能参考文献：
    1. analysis of the role of the Ceg1-carboxy terminal domain interaction in mRNA transcription PMID: 27503426
    2. The results indicate that the Cet1p-Ceg1p interaction is important not only for the activation of Ceg1p, but also for nuclear import of the complex. PMID: 24205062
    3. A crystal structure of Cet1-Ceg1 revealed a 176 kDa heterotetrameric complex composed of one Cet1 homodimer that associates with two Ceg1 molecules via interactions between the Ceg1 oligonucleotide binding domain and an extended Cet1 amino acid motif. PMID: 20159466
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Eukaryotic GTase family
  • 数据库链接：

    KEGG: sce:YGL130W

    STRING: 4932.YGL130W