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  4. Recombinant Salmonella typhimurium Deubiquitinase sseL (sseL)

Recombinant Salmonella typhimurium Deubiquitinase sseL (sseL)

  • 中文名称：
    鼠伤寒沙门菌sseL重组蛋白
  • 货号：
    CSB-YP838475SXB
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    鼠伤寒沙门菌sseL重组蛋白
  • 货号：
    CSB-EP838475SXB
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    鼠伤寒沙门菌sseL重组蛋白
  • 货号：
    CSB-EP838475SXB-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    鼠伤寒沙门菌sseL重组蛋白
  • 货号：
    CSB-BP838475SXB
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    鼠伤寒沙门菌sseL重组蛋白
  • 货号：
    CSB-MP838475SXB
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sseL
  • Uniprot No.：
  • 别名：
    sseL; STM2287Deubiquitinase SseL; EC 3.4.22.-; Deubiquitinating enzyme; DUB; Deubiquitinating protease; Salmonella secreted effector L
  • 种属：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-340
  • 氨基酸序列
    MNICVNSLYR LSIPQFHSLY TEEVSDEALT LLFSAVENGD QNCIDLLCNL ALRNDDLGHR VEKFLFDLFS GKRTGSSDID KKINQACLVL HQIANNDITK DNTEWKKLHA PSRLLYMAGS ATTDLSKKIG IAHKIMGDQF AQTDQEQVGV ENLWCGARML SSDELAAATQ GLVQESPLLS VNYPIGLIHP TTKENILSTQ LLEKIAQSGL SHNEVFLVNT GDHWLLCLFY KLAEKIKCLI FNTYYDLNEN TKQEIIEAAK IAGISESDEV NFIEMNLQNN VPNGCGLFCY HTIQLLSNAG QNDPATTLRE FAENFLTLSV EEQALFNTQT RRQIYEYSLQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protease targets the host cell ubiquitin pathway by acting as a deubiquitinase in infected host cells. Specifically hydrolyzes mono- and polyubiquitin substrates in vitro with a preference for 'Lys-63'-linked ubiquitin chains, suggesting that it interferes with a signaling pathway rather than inhibiting proteasomal-dependent degradation of its targets. Does not possess desumoylating activity. Is required for the Salmonella-induced delayed cytotoxicity in macrophages and full virulence. Is not required for intracellular bacterial replication.
  • 基因功能参考文献：
    1. SseL represents one of the first bacterial deubiquitinases demonstrated to modulate the host inflammatory response in vivo PMID: 18354230
    2. This protein is required for full virulence and the two-component regulatory system SsrA-SsrB for expression PMID: 17158898
    3. This gene is a ubiquitinase whose expression depends on the two-component regulatory system SsrA-SsrB PMID: 17360673
  • 亚细胞定位：
    Secreted. Host cytoplasm. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. In phagocytic cells localizes to the Salmonella-containing vacuole (SCV). In epithelial cells localizes to the Salmonella-containing vacuole (SCV) and to the Salmonella-induced filaments (Sifs), which are tubular membrane extensions from the SCV that are formed at late stages of infection.
  • 蛋白家族：
    Peptidase C79 family
  • 数据库链接：

    KEGG: stm:STM2287

    STRING: 99287.STM2287