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Recombinant Sheep Endothelin-1 (EDN1)

  • 中文名称：
    绵羊EDN1重组蛋白
  • 货号：
    CSB-YP858990SH
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    绵羊EDN1重组蛋白
  • 货号：
    CSB-EP858990SH
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    绵羊EDN1重组蛋白
  • 货号：
    CSB-EP858990SH-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    绵羊EDN1重组蛋白
  • 货号：
    CSB-BP858990SH
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    绵羊EDN1重组蛋白
  • 货号：
    CSB-MP858990SH
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    EDN1Endothelin-1; ET-1; Preproendothelin-1; PPET1) [Cleaved into: Big endothelin-1]
  • 种属：
    Ovis aries (Sheep)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    53-73
  • 氨基酸序列
    CSCSSLMD KECVYFCHLD IIW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
  • 基因功能参考文献：
    1. These results support the hypothesis that chronic hypoxia elicits sustained changes in fetal cerebrovascular reactivity to ET-1 through pathways dependent upon PKC, CaMKII, and p38 that cause increased ET-1-mediated contractility, decreased ET-1-mediated smooth muscle hypertrophy, and a depressed ability of ET-1 to promote contractile dedifferentiation. PMID: 28566491
    2. Endothelin receptor A, but not endothelin receptor B, mediates antisteroidogenic actions of endothelin 1 (EDN1) on prostaglandin (PGF2alpha)-induced luteolysis(endothelin 1). PMID: 18077801
    3. Study shows that ET-1 stimulates protein kinase Cd (PKCd) activity, which in turn attenuates eNOS transcription and NOS signaling through the activation of STAT3. PMID: 19754268
    4. Endothelin-1 has a role in reactive oxygen species production by pulmonary artery endothelial cells, leading to pulmonary artery endothelial cell dysfunction in congenital diaphragmatic hernia PMID: 25580737
    5. ET-1 decreases PPARgamma signaling and contributes to pulmonary artery endothelial cell dysfunction and impaired angiogenesis in persistent pulmonary hypertension of the newborn. PMID: 24337925
    6. Endothelin-1 impairs angiogenesis in vitro through Rho-kinase activation after chronic intrauterine pulmonary hypertension in fetal sheep. PMID: 23202724
    7. ET-1 stimulates ciliary beat frequency via interaction with endothelin A receptors; inhibition of both COX and NOS inhibited the effect of ET-1 suggesting a role for both enzymes in the ET-1 response downstream from the ETA-receptor PMID: 21664477

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Endothelin/sarafotoxin family
  • 数据库链接：

    KEGG: oas:443498

    UniGene: Oar.807